LOCUS       sequence-23254-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Sano T, Cantor CR
  TITLE     Intersubunit contacts made by tryptophan 120 with biotin are 
            essential for both strong biotin binding and biotin-induced tighter 
            subunit association of streptavidin.
  JOURNAL   Proc Natl Acad Sci U S A. 1995 Apr 11. 92(8):3180-4.
  PUBMED    7724536
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1995 Apr 11. 92(8):3180-4."
COMMENT     Sequence Label: pTSA-38
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             424..456
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(510..528)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      524..571
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttactttaag aaggagatat acatatgggc atcaccggca cctggtacaa ccagctcggc
       61 tcgaccttca tcgtgaccgc gggcgccgac ggcgccctga ccggaaccta ctagtcggcc
      121 gtcggcaacg ccgagagccg ctacgtcctg accggtcgtt acgacagcgc cccggccacc
      181 gacggcagcg gcaccgccct cggttggacg gtggcctgga agaataacta ccgcaacgcc
      241 cactccgcga ccacgtggag cggccagtac gtcggcggcg ccgaggcgag gatcaacacc
      301 cagtggctgc tgacctccgg caccaccgag gccaacgcct tcaagtccac gctggtcggc
      361 cacgacacct tcaccaaggt gtagccgtcc gccgcctcca tcgacgcggc gaagaaggcc
      421 catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccggctg ctaacaaagc
      481 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      541 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
//
