LOCUS       sequence-232555-       11675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rfp ""test"" strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1219
FEATURES             Location/Qualifiers
     source          1..11675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            8..116
                     /note="incP origin of transfer"
     rep_origin      385..773
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(1074..1865,1866..1934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1697..1716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2107..2125
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2163..2185)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2285..2304
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2718..2734
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2719..2735
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2763..2857
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      2960..3046
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3097..3144
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3808..3827
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3976..3981,3982..3998,3999..4004))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    4081..4128
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(4266..4943)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             4950..4961
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(5410..6492)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6454..6473
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6493..6570)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    6943..6959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(7001..7027,7028..7030,7031..9517,9518..9520,
                     9521..11104)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11114..11203
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     mobile_element  11359..11524
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
       61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
      121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
      181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
      241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa
      301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata
      361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
      661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
      721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
      781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa
      841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga cgacggtgaa
     2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt
     2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcaggtgtgg
     2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct
     2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg
     2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc
     3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta
     3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gttccagtcg
     3181 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3241 cgtattgggc gcatgcataa aaactgttgt aattcattaa gcattctgcc gacatggaag
     3301 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     3361 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     3421 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     3481 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     3541 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     3601 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     3661 ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     3721 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     3781 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     3841 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     3901 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tgagcgcata
     3961 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     4021 atccgtttcc acggctcgag agttcaacag acagccttcg ttcttatggc cgttcaggag
     4081 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttctt gagaagagaa
     4141 aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc
     4201 ttaagttttt tggctgaaac gaatgacgaa aaggctgtct gagcaaatcc aggaggtcgt
     4261 ttttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg
     4321 tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc
     4381 tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct
     4441 ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt
     4501 tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag
     4561 ttggtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg
     4621 gtaacggtaa caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc
     4681 gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa
     4741 ccgtactgga actgcgggga caggatgtcc caagcgaacg gcagcggacc acctttggta
     4801 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt
     4861 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg
     4921 ataacgtctt cgctactcgc catggtacct ttctcctctt taattaattc agatctatta
     4981 tacctaggac tgagctagct gtcaaattca ccaccctgaa ttgactctca aaccgtatta
     5041 gcccggtatt ttggaaatag cggaagcact gacttttgtt atcaataaaa aaggcccccc
     5101 gttagggagg ccttattgtt cgtctactcg gaagagcgag agacaacaga acggtcagcc
     5161 acatgaattc tttcagctca gtcgataggt agtaggcaag agtagtcgca cctttggtcg
     5221 aaaaaaaaag cccgcactgt caggtgcggg cttttttctg tgtttcccca aaagtaaaaa
     5281 cccgccgaag cgggttttta cgtaaaacag gtgaaactga ccagacgaga aggctttgga
     5341 ggtgataatg gggctcaagg accctggggt gcctaatgag tgagctaact tacattaatt
     5401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     5521 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     5581 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     5641 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
     5701 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     5761 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     5821 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     5881 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     5941 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     6001 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     6061 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     6121 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     6181 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     6241 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     6301 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     6361 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     6421 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     6481 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
     6541 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg catgccgctt gggaggcaga
     6601 taggtaggca tggcccccat tttcaataca agcaacgcat gagaaagccc ccggaagatc
     6661 accttccggg ggctttttta ttgcgctccg gcaattaaaa aagcggctaa ccacgccgct
     6721 ttttttacgt ctgcaggcga attgatctgg tttgacagct tatcaccaag ccagttacct
     6781 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     6841 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     6901 aatcagataa aatattataa atgtgagcgg ataacattga cattgtgagc ggataacaag
     6961 atactgagca catcagcagg tttcacacag gaaaactagt atggataaga aatactcaat
     7021 aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt
     7081 tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct
     7141 tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac
     7201 agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc
     7261 aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt
     7321 ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc
     7381 ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga
     7441 taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca
     7501 ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca
     7561 gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga
     7621 tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc
     7681 tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg
     7741 tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc
     7801 aaaagatact tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc
     7861 tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag
     7921 agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga
     7981 acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta
     8041 taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc
     8101 tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga
     8161 ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa
     8221 cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga
     8281 agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg
     8341 aatcccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg
     8401 gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc
     8461 agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt
     8521 actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt
     8581 caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc
     8641 cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga
     8701 ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt
     8761 taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt
     8821 ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga
     8881 agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt
     8941 gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat
     9001 taatggtatt agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg
     9061 ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga
     9121 cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt
     9181 agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt
     9241 ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa
     9301 tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg
     9361 tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca
     9421 aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga
     9481 attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac aaagtttcct
     9541 taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc
     9601 ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct
     9661 aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg
     9721 tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat
     9781 cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga
     9841 taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg
     9901 aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc
     9961 gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga
    10021 gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca
    10081 agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa
    10141 aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg
    10201 ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt
    10261 gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa
    10321 ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga
    10381 tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc
    10441 taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac
    10501 aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata
    10561 taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga
    10621 aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc
    10681 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg
    10741 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga
    10801 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt
    10861 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga
    10921 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt
    10981 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct
    11041 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg
    11101 tgacgcggcc gcggagcaga aactcatctc tgaagaagat ctggaacaaa agttgatttc
    11161 agaagaagat ctggaacaga agctcatctc tgaggaagat ctgtaataag gcgcgcctcc
    11221 ttaatgggac ttgcagcctc ggtaccaaat tccagaaaag acacccgaaa gggtgttttt
    11281 tcgttttggt cccacagaat gagcatcatg gctctagtcg acatcggaga agagtacggc
    11341 tcttttaacc gcctcaagaa ccagataagt gaaatctagt tccaaactat tttgtcattt
    11401 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
    11461 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
    11521 cacagcgggg catttttctt cctgttatgt ttgggcgctg cattaatgaa tcggccaacg
    11581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgacccgct
    11641 gcgctcggtc gttcggctgc ggcgagcggt atcag
//
