LOCUS       sequence-232561-       12711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rfp ""test"" strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 12711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1333
FEATURES             Location/Qualifiers
     source          1..12711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      32..126
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      229..315
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    366..413
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(546..1340)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     protein_bind    1915..1962
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2101..2778)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(3037..3066)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(3087..3162)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(3452..4078)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     3983..4002
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    4340..4358
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             join(4420..4446,4447..4449,4450..6936,6937..6939,
                     6940..8523)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8533..8622
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     oriT            9792..9900
                     /note="incP origin of transfer"
     rep_origin      10169..10557
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(10858..11649,11650..11718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11481..11500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11719..11823)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcgataagc tagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
       61 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      121 gagaatccaa gctagactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
      181 attggtgccc ttaaacgcct ggggtaatga ctctctagct tgaggcatca aataaaacga
      241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      301 ctgagtagga caaatccgcc gctaggagct tgcggcccgg acgatgagct cgaattgggg
      361 atcttgaagt acctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
      421 ttttgaagct gatgctcgag gatcctctag acatcagagt atggacagtt gcggatgtac
      481 ttcagaaaag attagatgtc taaaaagctt gtagttaaag ctttttagac atctaaatct
      541 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
      601 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
      661 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
      721 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
      781 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
      841 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
      901 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
      961 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     1021 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     1081 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     1141 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     1201 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     1261 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     1321 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     1381 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     1441 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     1501 cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt
     1561 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
     1621 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggca atatcttcta
     1681 tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
     1741 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
     1801 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
     1861 tctctagact cgagagttca acagacagcc ttcgttctta tggccgttca ggaggaagtt
     1921 cctattccga agttcctatt ctctagaaag tataggaact tcttgagaag agaaaagaaa
     1981 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt
     2041 tttttggctg aaacgaatga cgaaaaggct gtctgagcaa atccaggagg tcgtttaaac
     2101 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
     2161 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
     2221 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
     2281 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
     2341 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
     2401 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
     2461 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
     2521 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
     2581 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
     2641 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
     2701 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
     2761 gtcttcgcta ctcgccatag tagttcctcc ttatgtacta ttagttacat ttattgtaca
     2821 acacgagccc atttttgtca aataaaattt aaattatatc aacgttaata aggaattgat
     2881 aagcgtttaa accgtattag cccggtattt tggaaatagc ggaagcactg acttttgtta
     2941 tcaataaaaa aggccccccg ttagggaggc cttattgttc gtctactcgg aagagcgaga
     3001 gacaacagaa cggtcagcca catgaattcc gctattcaga tcctcttctg agatgagttt
     3061 ttgttcgggc ccaagcttca aaaaaagcac cgactcggtg ccactttttc aagttgataa
     3121 cggactagcc ttattttaac ttgctatttc tagctctaaa acagaccgct aaactgaaag
     3181 ttacatttat tgtacaacac gagcccattt ttgtcaaata aaatttaaat tatatcaacg
     3241 ttaataagga attctttcag ctcagtcgat aggtagtagg caagagtagt cgcacctttg
     3301 gtcgaaaaaa aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc cccaaaagta
     3361 aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa ctgaccagac gagaaggctt
     3421 tggaggtgat aatggggctc aaggacccgg gttaagaccc actttcacat ttaagttgtt
     3481 tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt
     3541 gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt
     3601 ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat
     3661 gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa
     3721 aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta
     3781 cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta
     3841 aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca
     3901 tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag
     3961 gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct
     4021 cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta
     4081 attcctcctt tttgttgaca ttatatcatt gatagagtta tttgtcaaac tattttttta
     4141 tttggatccc cccgggaggc agataggtag gcatggcccc cattttcaat acaagcaacg
     4201 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgct ccggcaatta
     4261 aaaaagcggc taaccacgcc gcttttttta cgtctgcaga ctagtgtcga gttcatgaaa
     4321 aactaaaaaa aatattgaca ctctatcatt gatagagtat aattaaaata agctctctat
     4381 cattgataga gtactaatag tacataagga ggaactagta tggataagaa atactcaata
     4441 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     4501 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     4561 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     4621 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     4681 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     4741 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     4801 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     4861 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     4921 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     4981 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     5041 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     5101 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     5161 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     5221 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     5281 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     5341 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     5401 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     5461 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     5521 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     5581 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     5641 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     5701 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     5761 atcccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     5821 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     5881 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     5941 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     6001 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     6061 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     6121 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     6181 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     6241 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     6301 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     6361 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     6421 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     6481 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     6541 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     6601 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     6661 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     6721 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     6781 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     6841 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     6901 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     6961 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     7021 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     7081 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     7141 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     7201 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     7261 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     7321 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     7381 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     7441 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     7501 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     7561 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     7621 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     7681 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     7741 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     7801 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     7861 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     7921 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     7981 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     8041 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     8101 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     8161 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     8221 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     8281 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     8341 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     8401 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     8461 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     8521 gacgcggccg cggagcagaa actcatctct gaagaagatc tggaacaaaa gttgatttca
     8581 gaagaagatc tggaacagaa gctcatctct gaggaagatc tgtaataagg cgcgcctcct
     8641 taatgggact tgcagcctcg gtaccaaatt ccagaaaaga cacccgaaag ggtgtttttt
     8701 cgttttggtc ccacagaatg agcatcatgg ctctagtcga ccgggtaggg gcatagctta
     8761 gataattgga aaagaggaaa aaagcttaat cttttttcga aggttaagct ttttctttta
     8821 tttataaaaa gtgaactaac tatcagaaag aaattatatt aaattttatt tttttgttta
     8881 aaaagtagat tatataaagg caagctaggt gggggaaaat atgtttaaaa aagaaaaagt
     8941 cacagaatac atttggacta tactaatacc aacaatcatc acttttatca ttagttgggt
     9001 tgggtcttat tacaatggta cttcgacagt tagtattgga caacctacaa aagtttccgg
     9061 tcagtatatc acgccaataa atataagtcc ctatcatgat attaaggaat taagaataac
     9121 ttttccgcaa aaactagatg taaaacaaat tagttcaaat gagcctataa atgtaaaatc
     9181 agataagaac aatataggag ttgaaagtaa ttccactttt gagattgcga aaatcgttga
     9241 aaataatagc gttcagttgc taattacaac acaaaaaaag ttaaacgata aggaaattag
     9301 aattgataaa aatggaaata acatttctgt aaattatgaa tctcagattg ttaatcctgc
     9361 aaaaaaacaa ttaatcaatc ttataattac gtcatctatt tattttataa tgcttaatat
     9421 actagcattg attatgaaca aaagatggga taagtattat gcaaaaatga aaaatgaaat
     9481 caaagaattt gaggataatg caaaagatct tgataaaaaa tcaaagaaga aaagcgagga
     9541 attatcggag ctgcgaaaga ccttgaacca agcgtttgag gaaactgata ggataaaata
     9601 tcatgagaag aaaaaacaaa tcctcctctt agctaagtta aacgattata aaaaagaact
     9661 aaccttttgg agaaatacaa taagaaaagt tctttatgaa cttcctgatg gagataaaaa
     9721 agcagataaa ctaataggga cagttacatc atctttaaaa acgtacggta cagtcgaaaa
     9781 gtaaagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     9841 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     9901 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     9961 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
    10021 tatgcagcgg aaagtatacc ttaaggaatc ccctgataac gcaggaaaga acatgtgagc
    10081 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgca ctccctgcct ctgtcatcac
    10141 gatactgtga tgccatggct aattcccatg tcagccgtta agtgttcctg tgtcactcaa
    10201 aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac
    10261 aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt
    10321 aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga
    10381 gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc
    10441 atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga gcttagtacg
    10501 tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct tcagatcctc
    10561 tacgccggac gcatcgtggc cggatcttgc ggccgcaaaa attaaaaatg aagttttaaa
    10621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10681 gcaccaataa ctgcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10981 tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag caataaacca
    11041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11821 gcgcacattt ccccgaaaag tgccacctga cgtcttctaa ctacttattt taaagcagtc
    11881 tggattgttt gggtaattca taaaaaaata aaagaaagaa ggaggaatag agtttttctt
    11941 ttttttgttt gcaatgttac tgtcaagtcg caaaagaatt gctatgaccg aaaaaaaacc
    12001 gcaaaggaat agatataagg tacctttttg caattcatct ttgtaaaata aaggttattc
    12061 tgacataata caattaatgt aaaaattcgc acaattttat gtaaggatgg gggaattttc
    12121 ttgcggggtg ttttcttaga taaagataaa attccgtacg acttagtcac gaaaaagtta
    12181 aatgaatggt atacatcaat aaaaaatgat caagttgagc aagccgagat tataaaaaca
    12241 gaagtagaga aagaattgtt aaacatggaa gaaaatcaag atgccctgtt atattatcaa
    12301 ctattagaat ttagacatga gataatgctg agttatatga aatctaagga aatagaagat
    12361 ctcaataatg cttatgagac tataaaagaa attgagaagc aagggcaatt aactggcatg
    12421 ttggaatact atttttactt ttttaagggt atgtacgagt ttaggcgtaa agaattaatt
    12481 tcagcgataa gtgcttatcg aatagctgaa tcaaagttgt cagaagttga ggatgaaata
    12541 gagaaagcag agtttttttt caaagtgtcc tatgtatatt attatatgaa acaaacatac
    12601 ttctccatga attatgcaaa tcgtgcactc aaaatattta gagagtatga agaatatgct
    12661 gtccagactg tgcgttgtca atttattgta gcaggaaact tgatcgagtg a
//
