LOCUS       sequence-232614-       12486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rfp ""test"" strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 12486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1335
FEATURES             Location/Qualifiers
     source          1..12486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..48
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(181..975)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     protein_bind    1550..1597
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1736..2413)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(2862..3488)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     3393..3412
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    3750..3768
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             join(3830..3856,3857..3859,3860..6346,6347..6349,
                     6350..7933)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7943..8032
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     oriT            9202..9310
                     /note="incP origin of transfer"
     rep_origin      9579..9967
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(10268..11059,11060..11128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10891..10910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11129..11233)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      12153..12247
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      12350..12436
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaagtaccta ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg
       61 aagctgatgc tcgaggatcc tctagacatc agagtatgga cagttgcgga tgtacttcag
      121 aaaagattag atgtctaaaa agcttgtagt taaagctttt tagacatcta aatctaggta
      181 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
      241 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
      301 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
      361 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
      421 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
      481 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
      541 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
      601 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
      661 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
      721 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
      781 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
      841 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
      901 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
      961 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     1021 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     1081 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     1141 gagccgattt tgaaaccaca attatgatag aatttacaag ctataaggtt attgtcctgg
     1201 gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata
     1261 agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcaatatc ttctatataa
     1321 aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc
     1381 ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa
     1441 ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttctct
     1501 agactcgaga gttcaacaga cagccttcgt tcttatggcc gttcaggagg aagttcctat
     1561 tccgaagttc ctattctcta gaaagtatag gaacttcttg agaagagaaa agaaaaccgc
     1621 cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt
     1681 ggctgaaacg aatgacgaaa aggctgtctg agcaaatcca ggaggtcgtt taaacttaag
     1741 caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt
     1801 tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt
     1861 ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct ttcagtttca
     1921 gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt tcggtggaag
     1981 cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag ttggtaccac
     2041 gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa
     2101 caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc gggaaggaca
     2161 gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa ccgtactgga
     2221 actgcgggga caggatgtcc caagcgaacg gcagcggacc acctttggta actttcagtt
     2281 tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt tcgaactcgt
     2341 gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg ataacgtctt
     2401 cgctactcgc catagtagtt cctccttatg tactattagt tacatttatt gtacaacacg
     2461 agcccatttt tgtcaaataa aatttaaatt atatcaacgt taataaggaa ttgataagcg
     2521 tttaaaccgt attagcccgg tattttggaa atagcggaag cactgacttt tgttatcaat
     2581 aaaaaaggcc ccccgttagg gaggccttat tgttcgtcta ctcggaagag cgagagacaa
     2641 cagaacggtc agccacatga attctttcag ctcagtcgat aggtagtagg caagagtagt
     2701 cgcacctttg gtcgaaaaaa aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc
     2761 cccaaaagta aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa ctgaccagac
     2821 gagaaggctt tggaggtgat aatggggctc aaggacccgg gttaagaccc actttcacat
     2881 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct
     2941 gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta
     3001 gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct
     3061 aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt
     3121 tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta
     3181 cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg
     3241 ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc
     3301 ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa
     3361 tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg
     3421 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta
     3481 gacatcatta attcctcctt tttgttgaca ttatatcatt gatagagtta tttgtcaaac
     3541 tattttttta tttggatccc cccgggaggc agataggtag gcatggcccc cattttcaat
     3601 acaagcaacg catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgct
     3661 ccggcaatta aaaaagcggc taaccacgcc gcttttttta cgtctgcaga ctagtgtcga
     3721 gttcatgaaa aactaaaaaa aatattgaca ctctatcatt gatagagtat aattaaaata
     3781 agctctctat cattgataga gtactaatag tacataagga ggaactagta tggataagaa
     3841 atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
     3901 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
     3961 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
     4021 caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
     4081 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
     4141 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
     4201 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
     4261 ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
     4321 tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact
     4381 atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag
     4441 tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa
     4501 tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt
     4561 gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt
     4621 acagctttca aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga
     4681 tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga
     4741 tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg
     4801 ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc
     4861 agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga
     4921 tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga
     4981 tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac
     5041 ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag
     5101 aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt
     5161 gacttttcga atcccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg
     5221 gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa
     5281 aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa
     5341 tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt
     5401 gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca
     5461 gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt
     5521 aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga
     5581 agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa
     5641 agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac
     5701 cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga
     5761 tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg
     5821 aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag attttttgaa
     5881 atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt
     5941 taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat
     6001 tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt
     6061 tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc
     6121 acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat
     6181 cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac
     6241 tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt
     6301 ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca
     6361 aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg
     6421 tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag
     6481 acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga
     6541 acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac
     6601 tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga
     6661 tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc
     6721 tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc
     6781 ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact
     6841 tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa
     6901 gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa
     6961 cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga
     7021 aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg
     7081 caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg
     7141 attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa
     7201 agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct
     7261 agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact
     7321 agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc
     7381 taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt
     7441 tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa
     7501 tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa
     7561 gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca
     7621 ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga
     7681 tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga
     7741 acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt
     7801 taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga
     7861 tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca
     7921 gctaggaggt gacgcggccg cggagcagaa actcatctct gaagaagatc tggaacaaaa
     7981 gttgatttca gaagaagatc tggaacagaa gctcatctct gaggaagatc tgtaataagg
     8041 cgcgcctcct taatgggact tgcagcctcg gtaccaaatt ccagaaaaga cacccgaaag
     8101 ggtgtttttt cgttttggtc ccacagaatg agcatcatgg ctctagtcga ccgggtaggg
     8161 gcatagctta gataattgga aaagaggaaa aaagcttaat cttttttcga aggttaagct
     8221 ttttctttta tttataaaaa gtgaactaac tatcagaaag aaattatatt aaattttatt
     8281 tttttgttta aaaagtagat tatataaagg caagctaggt gggggaaaat atgtttaaaa
     8341 aagaaaaagt cacagaatac atttggacta tactaatacc aacaatcatc acttttatca
     8401 ttagttgggt tgggtcttat tacaatggta cttcgacagt tagtattgga caacctacaa
     8461 aagtttccgg tcagtatatc acgccaataa atataagtcc ctatcatgat attaaggaat
     8521 taagaataac ttttccgcaa aaactagatg taaaacaaat tagttcaaat gagcctataa
     8581 atgtaaaatc agataagaac aatataggag ttgaaagtaa ttccactttt gagattgcga
     8641 aaatcgttga aaataatagc gttcagttgc taattacaac acaaaaaaag ttaaacgata
     8701 aggaaattag aattgataaa aatggaaata acatttctgt aaattatgaa tctcagattg
     8761 ttaatcctgc aaaaaaacaa ttaatcaatc ttataattac gtcatctatt tattttataa
     8821 tgcttaatat actagcattg attatgaaca aaagatggga taagtattat gcaaaaatga
     8881 aaaatgaaat caaagaattt gaggataatg caaaagatct tgataaaaaa tcaaagaaga
     8941 aaagcgagga attatcggag ctgcgaaaga ccttgaacca agcgtttgag gaaactgata
     9001 ggataaaata tcatgagaag aaaaaacaaa tcctcctctt agctaagtta aacgattata
     9061 aaaaagaact aaccttttgg agaaatacaa taagaaaagt tctttatgaa cttcctgatg
     9121 gagataaaaa agcagataaa ctaataggga cagttacatc atctttaaaa acgtacggta
     9181 cagtcgaaaa gtaaagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc
     9241 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     9301 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
     9361 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
     9421 gaagcagggt tatgcagcgg aaagtatacc ttaaggaatc ccctgataac gcaggaaaga
     9481 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgca ctccctgcct
     9541 ctgtcatcac gatactgtga tgccatggct aattcccatg tcagccgtta agtgttcctg
     9601 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
     9661 tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct
     9721 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
     9781 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag
     9841 tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga
     9901 gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct
     9961 tcagatcctc tacgccggac gcatcgtggc cggatcttgc ggccgcaaaa attaaaaatg
    10021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10081 aatcagtgag gcaccaataa ctgcctttga tcttttctac ggggtctgac gctcagtgga
    10141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10381 ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag
    10441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcttctaa ctacttattt
    11281 taaagcagtc tggattgttt gggtaattca taaaaaaata aaagaaagaa ggaggaatag
    11341 agtttttctt ttttttgttt gcaatgttac tgtcaagtcg caaaagaatt gctatgaccg
    11401 aaaaaaaacc gcaaaggaat agatataagg tacctttttg caattcatct ttgtaaaata
    11461 aaggttattc tgacataata caattaatgt aaaaattcgc acaattttat gtaaggatgg
    11521 gggaattttc ttgcggggtg ttttcttaga taaagataaa attccgtacg acttagtcac
    11581 gaaaaagtta aatgaatggt atacatcaat aaaaaatgat caagttgagc aagccgagat
    11641 tataaaaaca gaagtagaga aagaattgtt aaacatggaa gaaaatcaag atgccctgtt
    11701 atattatcaa ctattagaat ttagacatga gataatgctg agttatatga aatctaagga
    11761 aatagaagat ctcaataatg cttatgagac tataaaagaa attgagaagc aagggcaatt
    11821 aactggcatg ttggaatact atttttactt ttttaagggt atgtacgagt ttaggcgtaa
    11881 agaattaatt tcagcgataa gtgcttatcg aatagctgaa tcaaagttgt cagaagttga
    11941 ggatgaaata gagaaagcag agtttttttt caaagtgtcc tatgtatatt attatatgaa
    12001 acaaacatac ttctccatga attatgcaaa tcgtgcactc aaaatattta gagagtatga
    12061 agaatatgct gtccagactg tgcgttgtca atttattgta gcaggaaact tgatcgagtg
    12121 aatcgataag ctagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac
    12181 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
    12241 tgagaatcca agctagactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
    12301 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
    12361 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
    12421 cctgagtagg acaaatccgc cgctaggagc ttgcggcccg gacgatgagc tcgaattggg
    12481 gatctt
//
