LOCUS       sequence-232615-       11888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for cloning new sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1337
FEATURES             Location/Qualifiers
     source          1..11888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..48
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(181..975)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     protein_bind    1550..1597
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_RNA        complement(1885..1960)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(2264..2890)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     2795..2814
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    3152..3170
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             join(3232..3258,3259..3261,3262..5748,5749..5751,
                     5752..7335)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7345..7434
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     oriT            8604..8712
                     /note="incP origin of transfer"
     rep_origin      8981..9369
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(9670..10461,10462..10530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10293..10312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10531..10635)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      11555..11649
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      11752..11838
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaagtaccta ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg
       61 aagctgatgc tcgaggatcc tctagacatc agagtatgga cagttgcgga tgtacttcag
      121 aaaagattag atgtctaaaa agcttgtagt taaagctttt tagacatcta aatctaggta
      181 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
      241 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
      301 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
      361 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
      421 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
      481 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
      541 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
      601 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
      661 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
      721 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
      781 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
      841 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
      901 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
      961 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     1021 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     1081 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     1141 gagccgattt tgaaaccaca attatgatag aatttacaag ctataaggtt attgtcctgg
     1201 gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata
     1261 agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcaatatc ttctatataa
     1321 aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc
     1381 ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa
     1441 ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttctct
     1501 agactcgaga gttcaacaga cagccttcgt tcttatggcc gttcaggagg aagttcctat
     1561 tccgaagttc ctattctcta gaaagtatag gaacttcttg agaagagaaa agaaaaccgc
     1621 cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt
     1681 ggctgaaacg aatgacgaaa aggctgtctg agcaaatcca ggaggtcgtt taaaccgtat
     1741 tagcccggta ttttggaaat agcggaagca ctgacttttg ttatcaataa aaaaggcccc
     1801 ccgttaggga ggccttattg ttcgtctact cggaagagcg agagacaaca gaacggtcag
     1861 ccacatgaat tcctagtaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
     1921 gactagcctt attttaactt gctatttcta gctctaaaac cgagaccggg tctcccgaag
     1981 ggtctcgaca tttattgtac aacacgagcc catttttgtc aaataaaatt taaattatat
     2041 caacgttaat aaaagacgcg gaattctttc agctcagtcg ataggtagta ggcaagagta
     2101 gtcgcacctt tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt
     2161 tccccaaaag taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccag
     2221 acgagaaggc tttggaggtg ataatggggc tcaaggaccc gggttaagac ccactttcac
     2281 atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct
     2341 ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag
     2401 tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac
     2461 ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt
     2521 gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg
     2581 tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag
     2641 cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt
     2701 gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc
     2761 aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt
     2821 cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc
     2881 tagacatcat taattcctcc tttttgttga cattatatca ttgatagagt tatttgtcaa
     2941 actatttttt tatttggatc cccccgggag gcagataggt aggcatggcc cccattttca
     3001 atacaagcaa cgcatgagaa agcccccgga agatcacctt ccgggggctt ttttattgcg
     3061 ctccggcaat taaaaaagcg gctaaccacg ccgctttttt tacgtctgca gactagtgtc
     3121 gagttcatga aaaactaaaa aaaatattga cactctatca ttgatagagt ataattaaaa
     3181 taagctctct atcattgata gagtactaat agtacataag gaggaactag tatggataag
     3241 aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat
     3301 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc
     3361 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt
     3421 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag
     3481 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag
     3541 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta
     3601 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta
     3661 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag
     3721 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa
     3781 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca
     3841 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa
     3901 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct
     3961 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa
     4021 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga
     4081 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca
     4141 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa
     4201 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt
     4261 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt
     4321 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg
     4381 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg
     4441 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg
     4501 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc
     4561 ttgacttttc gaatccctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca
     4621 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat
     4681 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca
     4741 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa
     4801 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa
     4861 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa
     4921 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt
     4981 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat
     5041 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg
     5101 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt
     5161 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct
     5221 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg
     5281 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca
     5341 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat
     5401 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt
     5461 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg
     5521 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga
     5581 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat
     5641 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat
     5701 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca
     5761 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat
     5821 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg
     5881 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct
     5941 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa
     6001 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac
     6061 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt
     6121 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat
     6181 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa
     6241 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct
     6301 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg
     6361 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc
     6421 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg
     6481 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc
     6541 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa
     6601 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc
     6661 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta
     6721 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa
     6781 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt
     6841 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga
     6901 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa
     6961 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag
     7021 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca
     7081 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt
     7141 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct
     7201 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta
     7261 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt
     7321 cagctaggag gtgacgcggc cgcggagcag aaactcatct ctgaagaaga tctggaacaa
     7381 aagttgattt cagaagaaga tctggaacag aagctcatct ctgaggaaga tctgtaataa
     7441 ggcgcgcctc cttaatggga cttgcagcct cggtaccaaa ttccagaaaa gacacccgaa
     7501 agggtgtttt ttcgttttgg tcccacagaa tgagcatcat ggctctagtc gaccgggtag
     7561 gggcatagct tagataattg gaaaagagga aaaaagctta atcttttttc gaaggttaag
     7621 ctttttcttt tatttataaa aagtgaacta actatcagaa agaaattata ttaaatttta
     7681 tttttttgtt taaaaagtag attatataaa ggcaagctag gtgggggaaa atatgtttaa
     7741 aaaagaaaaa gtcacagaat acatttggac tatactaata ccaacaatca tcacttttat
     7801 cattagttgg gttgggtctt attacaatgg tacttcgaca gttagtattg gacaacctac
     7861 aaaagtttcc ggtcagtata tcacgccaat aaatataagt ccctatcatg atattaagga
     7921 attaagaata acttttccgc aaaaactaga tgtaaaacaa attagttcaa atgagcctat
     7981 aaatgtaaaa tcagataaga acaatatagg agttgaaagt aattccactt ttgagattgc
     8041 gaaaatcgtt gaaaataata gcgttcagtt gctaattaca acacaaaaaa agttaaacga
     8101 taaggaaatt agaattgata aaaatggaaa taacatttct gtaaattatg aatctcagat
     8161 tgttaatcct gcaaaaaaac aattaatcaa tcttataatt acgtcatcta tttattttat
     8221 aatgcttaat atactagcat tgattatgaa caaaagatgg gataagtatt atgcaaaaat
     8281 gaaaaatgaa atcaaagaat ttgaggataa tgcaaaagat cttgataaaa aatcaaagaa
     8341 gaaaagcgag gaattatcgg agctgcgaaa gaccttgaac caagcgtttg aggaaactga
     8401 taggataaaa tatcatgaga agaaaaaaca aatcctcctc ttagctaagt taaacgatta
     8461 taaaaaagaa ctaacctttt ggagaaatac aataagaaaa gttctttatg aacttcctga
     8521 tggagataaa aaagcagata aactaatagg gacagttaca tcatctttaa aaacgtacgg
     8581 tacagtcgaa aagtaaagct tatcggccag cctcgcagag caggattccc gttgagcacc
     8641 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     8701 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     8761 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     8821 ccgaagcagg gttatgcagc ggaaagtata ccttaaggaa tcccctgata acgcaggaaa
     8881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cactccctgc
     8941 ctctgtcatc acgatactgt gatgccatgg ctaattccca tgtcagccgt taagtgttcc
     9001 tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg
     9061 cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt
     9121 cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt
     9181 tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt
     9241 agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga
     9301 gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat
     9361 cttcagatcc tctacgccgg acgcatcgtg gccggatctt gcggccgcaa aaattaaaaa
     9421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9481 ttaatcagtg aggcaccaat aactgccttt gatcttttct acggggtctg acgctcagtg
     9541 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9601 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9661 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9721 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9781 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
     9841 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9901 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9961 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10021 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10081 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10141 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10201 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10261 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10321 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10381 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10441 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10501 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10561 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10621 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcttct aactacttat
    10681 tttaaagcag tctggattgt ttgggtaatt cataaaaaaa taaaagaaag aaggaggaat
    10741 agagtttttc ttttttttgt ttgcaatgtt actgtcaagt cgcaaaagaa ttgctatgac
    10801 cgaaaaaaaa ccgcaaagga atagatataa ggtacctttt tgcaattcat ctttgtaaaa
    10861 taaaggttat tctgacataa tacaattaat gtaaaaattc gcacaatttt atgtaaggat
    10921 gggggaattt tcttgcgggg tgttttctta gataaagata aaattccgta cgacttagtc
    10981 acgaaaaagt taaatgaatg gtatacatca ataaaaaatg atcaagttga gcaagccgag
    11041 attataaaaa cagaagtaga gaaagaattg ttaaacatgg aagaaaatca agatgccctg
    11101 ttatattatc aactattaga atttagacat gagataatgc tgagttatat gaaatctaag
    11161 gaaatagaag atctcaataa tgcttatgag actataaaag aaattgagaa gcaagggcaa
    11221 ttaactggca tgttggaata ctatttttac ttttttaagg gtatgtacga gtttaggcgt
    11281 aaagaattaa tttcagcgat aagtgcttat cgaatagctg aatcaaagtt gtcagaagtt
    11341 gaggatgaaa tagagaaagc agagtttttt ttcaaagtgt cctatgtata ttattatatg
    11401 aaacaaacat acttctccat gaattatgca aatcgtgcac tcaaaatatt tagagagtat
    11461 gaagaatatg ctgtccagac tgtgcgttgt caatttattg tagcaggaaa cttgatcgag
    11521 tgaatcgata agctagctta attagctgag cttggactcc tgttgataga tccagtaatg
    11581 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
    11641 ggtgagaatc caagctagac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
    11701 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
    11761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
    11821 ctcctgagta ggacaaatcc gccgctagga gcttgcggcc cggacgatga gctcgaattg
    11881 gggatctt
//
