LOCUS       sequence-232730-        3082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus YFP fused with silaffin-R5 silica affinity peptide 
            under the control of TetO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Olmez TT, Yuca E, Eyupoglu E, Catalak HB, Sahin O, Seker UOS
  TITLE     Autonomous Synthesis of Fluorescent Silica Biodots Using Engineered 
            Fusion Proteins.
  JOURNAL   ACS Omega. 2018 Jan 31;3(1):585-594. doi: 10.1021/acsomega.7b01769. 
            Epub 2018 Jan 18.
  PUBMED    30023783
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.7b01769"; journalName: "ACS Omega"; date: 
            "2018-01-31- 31"; volume: "3"; issue: "1"; pages: "585-594"
COMMENT     Sequence Label: pZA-YFP-R5
FEATURES             Location/Qualifiers
     source          1..3082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..19,20..25,26..42,43..48,49..74)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             109..120
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      957..1043
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1078..1095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1212..1757)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1292..1311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1871..1965)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1986..2645)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2560..2579
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2646..2748)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    2787..2805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2812..2830
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc
       61 aggacgcact gaccgaattc taccattcac ctcttggatt tgggtattaa agaggagaaa
      121 ggtaccatga gcaaaggtga agaactgttc accggcgttg tgccaattct ggttgagctg
      181 gatggtgacg tgaatggcca caaattttcc gtgtctggtg aaggcgaggg tgatgctact
      241 tatggcaaac tgactctgaa actgatctgt accaccggca aactgcctgt tccgtggcca
      301 actctggtca ctactctggg ttacggcctg atgtgttttg cgcgttaccc ggatcacatg
      361 aaacagcatg acttcttcaa atctgccatg ccggaaggct atgtccaaga acgtacgatc
      421 tttttcaagg acgacggcaa ctataaaacc cgtgccgaag ttaaattcga gggtgacacc
      481 ctggttaacc gcatcgaact gaaaggcatt gacttcaaag aggacggcaa cattctgggt
      541 cacaagctgg aatacaacta caactcccac aacgtttaca ttactgctga caagcagaaa
      601 aacggcatca aagcaaactt caagatccgt cacaacattg aagatggtgg cgtacagctg
      661 gcagatcact accagcagaa cactccaatc ggtgatggcc cagtactgct gccagataac
      721 cattacctgt cctaccagag caaactgtct aaagacccga acgaaaaacg tgaccacatg
      781 gtactgctgg aatttgttac cgcggcaggc attacccacg gtatggacga actgtataaa
      841 ggtggcggtg gcagtggcgg cggcggtagc ggcgggggag gatctagcag caaaaaaagc
      901 ggcagctata gcggcagcaa aggcagcaaa cgccgcattc tgtaaacgcg tggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcct gagtaggaca aatccgccgc cctagaccta gggatatatt ccgcttcctc
     1081 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     1141 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     1201 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     1261 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     1321 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1381 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1441 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     1501 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     1561 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     1621 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     1681 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     1741 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     1801 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgttac
     1861 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1921 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1981 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     2041 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2101 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2161 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2221 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2281 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2341 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2401 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     2461 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2521 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2581 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2641 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     2701 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     2761 cgccagatat cgacgtcaaa gggccctccc tatcagtgat agagattgac atccctatca
     2821 gtgatagaga tactgagcac agtcgacacc caaatccagg aggtgattgg tagtggtggt
     2881 taatgaaaat taacttacta ctaccatata tctctagatg cctggcggca gtagcgcggt
     2941 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3001 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     3061 cgaaagactg ggccttctcg ag
//
