LOCUS       sequence-232749-       14992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhiC31 integration plasmid for tetO array with Neo selection 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, 
            Lomvardas S, Weiner OD
  TITLE     Live-cell imaging reveals enhancer-dependent Sox2 transcription in 
            the absence of enhancer proximity.
  JOURNAL   Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
  PUBMED    31124784
REFERENCE   2  (bases 1 to 14992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-24"; volume: "8. pii: 41769. doi"; pages: " 
            10.7554/eLife.41769"
COMMENT     Sequence Label: pDEST-tetOx224_PhiC31attB_FRT-PGKneo-FRT
FEATURES             Location/Qualifiers
     source          1..14992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(166..184,185..207,208..226,227..249,250..268,269..291,
                     292..310,311..333,334..352,353..375,376..394,395..417,
                     418..437)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(208..226,227..249,250..268,269..291,292..310,311..333,
                     334..352,353..375,376..394,395..417,418..437,438..460,
                     461..479)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(250..268,269..291,292..310,311..333,334..352,353..375,
                     376..394,395..417,418..437,438..460,461..479,480..502,
                     503..521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(292..310,311..333,334..352,353..375,376..394,395..417,
                     418..437,438..460,461..479,480..502,503..521,522..544,
                     545..563)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(334..352,353..375,376..394,395..417,418..437,438..460,
                     461..479,480..502,503..521,522..544,545..563,564..586,
                     587..605)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(682..700,701..723,724..742,743..765,766..784,785..807,
                     808..826,827..849,850..868,869..891,892..910,911..933,
                     934..952)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1029..1047,1048..1070,1071..1089,1090..1112,1113..1131,
                     1132..1154,1155..1173,1174..1196,1197..1215,1216..1238,
                     1239..1257,1258..1280,1281..1299)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1376..1394,1395..1417,1418..1436,1437..1459,1460..1478,
                     1479..1501,1502..1520,1521..1543,1544..1562,1563..1585,
                     1586..1604,1605..1627,1628..1646)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1723..1741,1742..1764,1765..1783,1784..1806,1807..1825,
                     1826..1848,1849..1867,1868..1890,1891..1909,1910..1932,
                     1933..1951,1952..1974,1975..1993)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2070..2088,2089..2111,2112..2130,2131..2153,2154..2172,
                     2173..2195,2196..2214,2215..2237,2238..2256,2257..2279,
                     2280..2298,2299..2321,2322..2340)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2417..2435,2436..2458,2459..2477,2478..2500,2501..2519,
                     2520..2542,2543..2561,2562..2584,2585..2603,2604..2626,
                     2627..2645,2646..2668,2669..2687)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2764..2782,2783..2805,2806..2824,2825..2847,2848..2866,
                     2867..2889,2890..2908,2909..2931,2932..2950,2951..2973,
                     2974..2992,2993..3015,3016..3034)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3111..3129,3130..3152,3153..3171,3172..3194,3195..3213,
                     3214..3236,3237..3255,3256..3278,3279..3297,3298..3320,
                     3321..3339,3340..3362,3363..3381)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3458..3476,3477..3499,3500..3518,3519..3541,3542..3560,
                     3561..3583,3584..3602,3603..3625,3626..3644,3645..3667,
                     3668..3686,3687..3709,3710..3728)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3805..3823,3824..3846,3847..3865,3866..3888,3889..3907,
                     3908..3930,3931..3949,3950..3972,3973..3991,3992..4014,
                     4015..4033,4034..4056,4057..4075)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4152..4170,4171..4193,4194..4212,4213..4235,4236..4254,
                     4255..4277,4278..4296,4297..4319,4320..4338,4339..4361,
                     4362..4380,4381..4403,4404..4422)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4499..4517,4518..4540,4541..4559,4560..4582,4583..4601,
                     4602..4624,4625..4643,4644..4666,4667..4685,4686..4708,
                     4709..4727,4728..4750,4751..4769)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4846..4864,4865..4887,4888..4906,4907..4929,4930..4948,
                     4949..4971,4972..4990,4991..5013,5014..5032,5033..5055,
                     5056..5074,5075..5097,5098..5116)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5193..5211,5212..5234,5235..5253,5254..5276,5277..5295,
                     5296..5318,5319..5337,5338..5360,5361..5379,5380..5402,
                     5403..5421,5422..5444,5445..5463)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5540..5558,5559..5581,5582..5600,5601..5623,5624..5642,
                     5643..5665,5666..5684,5685..5707,5708..5726,5727..5749,
                     5750..5768,5769..5791,5792..5810)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5887..5905,5906..5928,5929..5947,5948..5970,5971..5989,
                     5990..6012,6013..6031,6032..6054,6055..6073,6074..6096,
                     6097..6115,6116..6138,6139..6157)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6234..6252,6253..6275,6276..6294,6295..6317,6318..6336,
                     6337..6359,6360..6378,6379..6401,6402..6420,6421..6443,
                     6444..6462,6463..6485,6486..6504)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6581..6599,6600..6622,6623..6641,6642..6664,6665..6683,
                     6684..6706,6707..6725,6726..6748,6749..6767,6768..6790,
                     6791..6809,6810..6832,6833..6851)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6928..6946,6947..6969,6970..6988,6989..7011,7012..7030,
                     7031..7053,7054..7072,7073..7095,7096..7114,7115..7137,
                     7138..7156,7157..7179,7180..7198)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7275..7293,7294..7316,7317..7335,7336..7358,7359..7377,
                     7378..7400,7401..7419,7420..7442,7443..7461,7462..7484,
                     7485..7503,7504..7526,7527..7545)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7622..7640,7641..7663,7664..7682,7683..7705,7706..7724,
                     7725..7747,7748..7766,7767..7789,7790..7808,7809..7831,
                     7832..7850,7851..7873,7874..7892)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7969..7987,7988..8010,8011..8029,8030..8052,8053..8071,
                     8072..8094,8095..8113,8114..8136,8137..8155,8156..8178,
                     8179..8197,8198..8220,8221..8239)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8316..8334,8335..8357,8358..8376,8377..8399,8400..8418,
                     8419..8441,8442..8460,8461..8483,8484..8502,8503..8525,
                     8526..8544,8545..8567,8568..8586)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8663..8681,8682..8704,8705..8723,8724..8746,8747..8765,
                     8766..8788,8789..8807,8808..8830,8831..8849,8850..8872,
                     8873..8891,8892..8914,8915..8933)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9010..9028,9029..9051,9052..9070,9071..9093,9094..9112,
                     9113..9135,9136..9154,9155..9177,9178..9196,9197..9219,
                     9220..9238,9239..9261,9262..9280)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9357..9375,9376..9398,9399..9417,9418..9440,9441..9459,
                     9460..9482,9483..9501,9502..9524,9525..9543,9544..9566,
                     9567..9585,9586..9608,9609..9627)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(9660..9676)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9676..9700)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     9724..9740
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9777..9793)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10011..10080
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(10577..10594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(10692..10725)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(10786..11374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10866..10885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11545..12336,12337..12405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12168..12187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12406..12510)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12537..12992
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12624..12643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12834..12855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13118..13140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13132..13149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13133..13149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    13219..13443
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13432..13449
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(13481..14284)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13592..13611)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14202..14221
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(14303..14350)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(14362..14861)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(14410..14429)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     14803..14826
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(14925..14958)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    join(14992,1..24)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
       61 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      121 ggatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      361 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccacttc
      421 cntatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      481 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      541 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      601 agagaaaagt gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg aggccctttc
      661 gtcttcactc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      721 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      781 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      841 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      901 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      961 agtcgggctc ggttcccggg tcgagatccg tatcacgagg ccctttcgtc ttcactcgag
     1021 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     1081 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     1141 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     1201 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     1261 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgggctcggt
     1321 tcccgggtcg agatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct
     1381 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1441 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     1501 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     1561 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     1621 ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga
     1681 tccgtatcac gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag
     1741 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1801 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1861 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1921 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     1981 tcagtgatag agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag
     2041 gccctttcgt cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2101 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2161 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2221 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2281 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2341 aaagtgaaag tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt
     2401 cactcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2461 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2521 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2581 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2641 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2701 ggctcggttc ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac
     2761 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2821 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2881 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2941 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     3001 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg
     3061 ggtcgagatc cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag
     3121 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     3181 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     3241 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     3301 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     3361 actccctatc agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt
     3421 atcacgaggc cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag
     3481 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3541 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3601 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     3661 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     3721 gatagagaaa agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct
     3781 ttcgtcttca ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     3841 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     3901 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     3961 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     4021 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     4081 gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg aggccctttc gtcttcactc
     4141 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4201 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4261 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4321 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4381 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc
     4441 ggttcccggg tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc
     4501 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     4561 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     4621 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     4681 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     4741 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg
     4801 agatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata
     4861 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     4921 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     4981 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     5041 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     5101 ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac
     5161 gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5221 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     5281 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     5341 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     5401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5461 agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt
     5521 cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     5581 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     5641 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     5701 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     5761 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     5821 tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt
     5881 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     5941 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     6001 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     6061 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     6121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc
     6181 ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat
     6241 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     6301 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     6361 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     6421 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     6481 accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc
     6541 cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa
     6601 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     6661 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     6721 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     6781 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     6841 agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc
     6901 cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     6961 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     7021 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     7081 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     7141 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     7201 agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct ttcgtcttca
     7261 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     7321 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     7381 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     7441 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     7501 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcggg
     7561 ctcggttccc gggtcgagat ccgtatcacg aggccctttc gtcttcactc gagtttacca
     7621 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     7681 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     7741 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     7801 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     7861 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc ggttcccggg
     7921 tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc cctatcagtg
     7981 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     8041 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     8101 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     8161 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     8221 tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat
     8281 cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg
     8341 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     8401 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     8461 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     8521 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     8581 tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt
     8641 cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     8701 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     8761 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     8821 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     8881 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     8941 aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga
     9001 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     9061 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     9121 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     9181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     9241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg
     9301 ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc
     9361 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     9421 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     9481 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     9541 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     9601 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag
     9661 atccactagt tctagaccca gctttcttgt acaaagtggt tgatggggat ccgaattcga
     9721 gctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggcagggatc
     9781 cactagttct agaattcgag ctcccgggta ctcgaggtcg acatgcccgc cgtgaccgtc
     9841 gagaacccgc tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg
     9901 ctcaccgtga ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg
     9961 ttcgccggga tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag
    10021 gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag
    10081 accgtgacct acatcgtcga cgcggccaga tggtaagccc tcccgtatcg tagttatcta
    10141 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10201 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10261 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10321 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10381 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10441 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10501 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtattg
    10561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10681 gaaagaacat ggaagttcct atactttcta gagaatagga acttccatgt gagcaaaagg
    10741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11221 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
    12541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    12601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    12661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    12721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    12781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    12841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    12901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    12961 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt
    13021 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    13081 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    13141 acggccagtg aattcgagct cggtacaggg ttccgcaagc tctagtcgag ccccagctgg
    13201 ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt
    13261 cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac
    13321 tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt
    13381 ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca
    13441 cagtcgaggc tgatcagcga gctctagaga attgatcccc tcagaagaac tcgtcaagaa
    13501 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
    13561 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
    13621 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
    13681 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatca tcgccgtcgg
    13741 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
    13801 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
    13861 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
    13921 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
    13981 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
    14041 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
    14101 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
    14161 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
    14221 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atggccgatc
    14281 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
    14341 aattgtcaac aggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc
    14401 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
    14461 cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc
    14521 ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca
    14581 ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca
    14641 cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga
    14701 aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga
    14761 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
    14821 gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tcgacgacct
    14881 gcagccaagc tagcttggct ggacgtaaac tcctcttcag acctgaagtt cctatacttt
    14941 ctagagaata ggaacttcgg aattcgatat caagcgtacc gggccccatc aa
//
