LOCUS       sequence-232750-        9630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mTurq2 tagged PCNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE
  TITLE     Accurate delineation of cell cycle phase transitions in living cells
            with PIP-FUCCI.
  JOURNAL   Cell Cycle. 2018;17(21-22):2496-2516. doi: 
            10.1080/15384101.2018.1547001.
  PUBMED    30421640
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2018"; volume: "17(21-22):2496-2516. doi"; pages: " 
            10.1080/15384101.2018.1547001"
COMMENT     Sequence Label: pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    132..153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(168..173,174..191,192..198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    206..222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     211..233
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     230..246
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     230..246
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     265..285
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        267..285
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        313..539
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             540..720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    767..892
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1385..1618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1803..1847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2114..2231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2277..2787
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2706..2724
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2842..2866
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     5'UTR           2869..2908
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             2944..2964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      2977..2986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2983..2985,2986..2988,2989..3699)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3028..3049)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3289..3308)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3636..3657
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4547..4571)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4602..5190
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4655..4675)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5073..5084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5204..5703
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5239..5262)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5636..5655
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5724..6323
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5724..5743)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6220..6240
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6461..6694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6766..6887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6803..6822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6857..6876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6927..7062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7082..7101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7083..7101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7111..7128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7111..7127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7120..7142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7269..7724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7356..7375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7566..7587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7750..7854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7855..7923,7924..8715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8073..8092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8886..9474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9375..9394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9628..9630,1..15)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
       61 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      121 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      181 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      241 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
      301 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
      361 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      421 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      481 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      541 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      601 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      661 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      721 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      781 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      841 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      901 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      961 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1021 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1081 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1141 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1201 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1261 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1321 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1381 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1441 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1501 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1561 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1621 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1681 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1741 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1801 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1861 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1921 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1981 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2041 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     2101 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2221 ttcaaaattt tatcgatcac gagactagcc tcgagaagct tgatatcgaa ttccacgggg
     2281 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2341 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2401 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2461 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     2521 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     2581 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     2641 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     2701 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     2761 gaccgaatca ccgacctctc tccccagggg gatccaccgg ttcgtcgact agtccagtgt
     2821 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggcttcga tcttggtggc
     2881 gtgaaactcc cgcacctctt cggccagcgc cttgtagaag cgcgtatggc ttcgtgggga
     2941 tccccgaaga agaagcgcaa agtactggta ccggtcgcca ccatggtgag caagggcgag
     3001 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3061 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3121 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc
     3181 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     3241 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3301 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3361 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3421 tttagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     3481 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     3541 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3601 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3661 gccgccggga tcactctcgg catggacgag ctgtacaagg ggcgccgcga agggcaaggg
     3721 caagggcaag ggccgggccg cggctacgcg tatcgatcca tgttcgaggc gcgcctggtc
     3781 cagggctcca tcctcaagaa ggtgttggag gcactcaagg acctcatcaa cgaggcctgc
     3841 tgggatatta gctccagcgg tgtaaacctg cagagcatgg actcgtccca cgtctctttg
     3901 gtgcagctca ccctgcggtc tgagggcttc gacacctacc gctgcgaccg caacctggcc
     3961 atgggcgtga acctcaccag tatgtccaaa atactaaaat gcgccggcaa tgaagatatc
     4021 attacactaa gggccgaaga taacgcggat accttggcgc tagtatttga agcaccaaac
     4081 caggagaaag tttcagacta tgaaatgaag ttgatggatt tagatgttga acaacttgga
     4141 attccagaac aggagtacag ctgtgtagta aagatgcctt ctggtgaatt tgcacgtata
     4201 tgccgagatc tcagccatat tggagatgct gttgtaattt cctgtgcaaa agacggagtg
     4261 aaattttctg caagtggaga acttggaaat ggaaacatta aattgtcaca gacaagtaat
     4321 gtcgataaag aggaggaagc tgttaccata gagatgaatg aaccagttca actaactttt
     4381 gcactgaggt acctgaactt ctttacaaaa gccactccac tctcttcaac ggtgacactc
     4441 agtatgtctg cagatgtacc ccttgttgta gagtataaaa ttgcggatat gggacactta
     4501 aaatactact tggctcccaa gatcgaggat gaagaaggat cttaggaccc agctttcttg
     4561 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
     4621 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4681 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4741 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4801 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4861 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4981 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5041 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5101 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5161 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     5221 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     5281 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     5341 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     5401 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5461 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5521 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     5581 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5641 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5701 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5761 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5821 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5881 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5941 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6001 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6061 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6121 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6181 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6241 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6301 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     6361 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     6421 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     6481 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6661 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6721 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6841 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6901 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6961 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7021 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7081 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7141 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7261 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7681 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9601 gctcgccgca gccgaacgac cgagcgcagc
//
