LOCUS       sequence-232755-       13683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homologous recombination vector for dox-inducible overexpression of 
            OTX2, PAX6, and MITF in AAVS locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13683)
  TITLE     TF-induced RPE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVS hOPM
FEATURES             Location/Qualifiers
     source          1..13683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..804
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             join(858..926,927..1718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1076..1095)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1889..2477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2378..2397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2525..3361
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    3394..3412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3430..3448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3466..3484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3502..3520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3538..3556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3574..3592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3610..3628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3673..3697
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4689..4742
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6060..6113
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    7702..7823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7739..7758)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7793..7812
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7872..7888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7872..7888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7885..7907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7896..7912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7920..7926,7927..7944,7945..7950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7965..7986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8103..8120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8412..8431
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    8430..8485
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8531..8550
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(8641..9387)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9419..9438)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          9446..10462
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(9500..9522)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(10463..10740)
                     /label=chicken beta-actin promoter
     enhancer        10742..11121
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    11127..11351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     11340..11357
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    11373..11406
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11682..11701)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11951..11969
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      12111..12120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             12158..12426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(12439..12650)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    12686..12910
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12931..13530)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13014..13034)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     13511..13530
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(13540..13593)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    13617..13642
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    13649..13682
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccccactgt ggggtggagg ggacagataa aagtacccag aaccagagcc acattaaccg
       61 gccctgggaa tataaggtgg tcccagctcg gggacacagg atccctggag gcagcaaaca
      121 tgctgtcctg aagtggacat aggggcccgg gttggaggaa gaagactagc tgagctctcg
      181 gacccctgga agatgccatg acagggggct ggaagagcta gcacagacta gagaggtaag
      241 gggggtaggg gagctgccca aatgaaagga gtgagaggtg acccgaatcc acaggagaac
      301 ggggtgtcca ggcaaagaaa gcaagaggat ggagaggtgg ctaaagccag ggagacgggg
      361 tactttgggg ttgtccagaa aaacggtgat gatgcaggcc tacaagaagg ggaggcggga
      421 cgcaagggag acatccgtcg gagaaggcca tcctaagaaa cgagagatgg cacaggcccc
      481 agaaggagaa ggaaaaggga acccagcgag tgaagacggc atggggttgg gtgagggagg
      541 agagatgccc ggagaggacc cagacacggg gaggatccgc tcagaggaca tcacgtggtg
      601 cagcgccgag aaggaagtgc tccggaaaga gcatccttgg gcagcaacac agcagagagc
      661 aaggggaaga gggagtggag gaagacggaa cctgaaggag gcggcaggga aggatctggg
      721 ccagccgtag aggtgaccca ggccacaagc tgcagacaga aagcggcaca ggcccagggg
      781 agagaatgct ggtcagagaa agcaaagggc gaattcaacc ctgataaatg cttcaataat
      841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1081 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1381 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1501 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1561 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1621 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1681 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gcgaattcgc
     2521 ccttagagca gagccaggaa cccctgtagg gaaggggcag gagagccagg ggcatgagat
     2581 ggtggacgag gaagggggac agggaagcct gagcgcctct cctgggcttg ccaaggactc
     2641 aaacccagaa gcccagagca gggccttagg gaagcgggac cctgctctgg gcggaggaat
     2701 atgtcccaga tagcactggg gactctttaa ggaaagaagg atggagaaag agaaagggag
     2761 tagaggcggc cacgacctgg tgaacaccta ggacgcacca ttctcacaaa gggagttttc
     2821 cacacggaca cccccctcct caccacagcc ctgccaggac ggggctggct actggcctta
     2881 tctcacaggt aaaactgacg cacggaggaa caatataaat tggggactag aaaggtgaag
     2941 agccaaagtt agaactcagg accaacttat tctgattttg tttttccaaa ctgcttctcc
     3001 tcttgggaag tgtaaggaag ctgcagcacc aggatcagtg aaacgcacca gacagccgcg
     3061 tcagagcagc tcaggttctg ggagagggta gcgcagggtg gccactgaga accgggcagg
     3121 tcacgcatcc cccccttccc tcccaccccc tgccaagctc tccctcccag gatcctctct
     3181 ggctccatcg taagcaaacc ttagaggttc tggcaaggag agagatggct ccaggaaatg
     3241 ggggtgtgtc accagataag gaatctgcct aacaggaggt gggggttaga cccaatatca
     3301 ggagactagg aaggaggagg cctaaggatg gggcttttct gtcaccaatc ctgtccctag
     3361 taaagctgat atctccaccc cacagtggtt tactccctat cagtgataga gaacgtatga
     3421 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     3481 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     3541 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     3601 ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc
     3661 ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact
     3721 tttgtcttat accaactttc cgtaccactt cctaccctcg taaacttaag gttaattaag
     3781 ccgccaccat gatgtcttat cttaagcaac cgccttacgc agtcaatggg ctgagtctga
     3841 ccacttcggg tatggacttg ctgcacccct ccgtgggcta cccggggccc tgggcttctt
     3901 gtcccgcagc caccccccgg aaacagcgcc gggagaggac gacgttcact cgggcgcagc
     3961 tagatgtgct ggaagcactg tttgccaaga cccggtaccc agacatcttc atgcgagagg
     4021 aggtggcact gaaaatcaac ttgcccgagt cgagggtgca ggtatggttt aagaatcgaa
     4081 gagctaagtg ccgccaacaa cagcaacaac agcagaatgg aggtcaaaac aaagtgagac
     4141 ctgccaaaaa gaagacatct ccagctcggg aagtgagttc agagagtgga acaagtggcc
     4201 aattcactcc cccctctagc acctcagtcc cgaccattgc cagcagcagt gctcctgtgt
     4261 ctatctggag cccagcttcc atctccccac tgtcagatcc cttgtccacc tcctcttcct
     4321 gcatgcagag gtcctatccc atgacctata ctcaggcttc aggttatagt caaggatatg
     4381 ctggctcaac ttcctacttt gggggcatgg actgtggatc atatttgacc cctatgcatc
     4441 accagcttcc cggaccaggg gccacactca gtcccatggg taccaatgca gtcaccagcc
     4501 atctcaatca gtccccagct tctctttcca cccagggata tggagcttca agcttgggtt
     4561 ttaactcaac cactgattgc ttggattata aggaccaaac tgcctcctgg aagcttaact
     4621 tcaatgctga ctgcttggat tataaagatc agacatcctc gtggaaattc caggttttgg
     4681 gatcgggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
     4741 caatgcagaa cagtcacagc ggagtgaatc agctcggtgg tgtctttgtc aacgggcggc
     4801 cactgccgga ctccacccgg cagaagattg tagagctagc tcacagcggg gcccggccgt
     4861 gcgacatttc ccgaattctg cagacccatg cagatgcaaa agtccaagtg ctggacaatc
     4921 aaaacgtgtc caacggatgt gtgagtaaaa ttctgggcag gtattacgag actggctcca
     4981 tcagacccag ggcaatcggt ggtagtaaac cgagagtagc gactccagaa gttgtaagca
     5041 aaatagccca gtataagcgg gagtgcccgt ccatctttgc ttgggaaatc cgagacagat
     5101 tactgtccga gggggtctgt accaacgata acataccaag cgtgtcatca ataaacagag
     5161 ttcttcgcaa cctggctagc gaaaagcaac agatgggcgc agacggcatg tatgataaac
     5221 taaggatgtt gaacgggcag accggaagct ggggcacccg ccctggttgg tatccgggga
     5281 cttcggtgcc agggcaacct acgcaagatg gctgccagca acaggaagga gggggagaga
     5341 ataccaactc catcagttcc aacggagaag attcagatga ggctcaaatg cgacttcagc
     5401 tgaagcggaa gctgcaaaga aatagaacat cctttaccca agagcaaatt gaggccctgg
     5461 agaaagagtt tgagagaacc cattatccag atgtgtttgc ccgagaaaga ctagcagcca
     5521 aaatagatct acctgaagca agaatacagg tatggttttc taatcgaagg gccaaatgga
     5581 gaagagaaga aaaactgagg aatcagagaa gacaggccag caacacacct agtcatattc
     5641 ctatcagcag tagtttcagc accagtgtct accaaccaat tccacaaccc accacaccgg
     5701 tttcctcctt cacatctggc tccatgttgg gccgaacaga cacagccctc acaaacacct
     5761 acagcgctct gccgcctatg cccagcttca ccatggcaaa taacctgcct atgcaacccc
     5821 cagtccccag ccagacctcc tcatactcct gcatgctgcc caccagccct tcggtgaatg
     5881 ggcggagtta tgatacctac acccccccac atatgcagac acacatgaac agtcagccaa
     5941 tgggcacctc gggcaccact tcaacaggac tcatttcccc tggtgtgtca gttccagttc
     6001 aagttcccgg aagtgaacct gatatgtctc aatactggcc aagattacag ggatcgggag
     6061 agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatgcagt
     6121 ccgaatcggg gatcgtgccg gatttcgaag tcggggagga gtttcatgaa gagcccaaaa
     6181 cctattacga actcaaaagt caaccgctga agagcagcag ttccgccgag catcctgggg
     6241 cctccaagcc tccgataagc tcctccagta tgacatcacg catcttgcta cgccagcaac
     6301 tcatgcgtga gcagatgcag gagcaggagc gcagggagca gcagcagaag ctgcaggcgg
     6361 cccagttcat gcaacagaga gtgcccgtga gtcagacacc agccataaac gtcagtgtgc
     6421 ccaccaccct tccctctgcc acgcaggtgc cgatggaagt ccttaaggtg cagacccacc
     6481 tcgaaaaccc caccaagtac cacatacagc aagcccaacg gcagcaggta aagcagtacc
     6541 tttctaccac tttagcaaat aaacatgcca accaagtcct gagcttgcca tgtccaaacc
     6601 agcctggcga tcatgtcatg ccaccggtgc cggggagcag cgcacccaac agccccatgg
     6661 ctatgcttac gcttaactcc aactgtgaaa aagagggatt ttataagttt gaagagcaaa
     6721 acagggcaga gagcgagtgc ccaggcatga acacacattc acgagcgtcc tgtatgcaga
     6781 tggatgatgt aatcgatgac atcattagcc tagaatcaag ttataatgag gaaatcttgg
     6841 gcttgatgga tcctgctttg caaatggcaa atacgttgcc tgtctcggga aacttgattg
     6901 atctttatgg aaaccaaggt ctgcccccac caggcctcac catcagcaac tcctgtccag
     6961 ccaaccttcc caacataaaa agggagctca cagagtctga agcaagagca ctggccaaag
     7021 agaggcagaa aaaggacaat cacaacctga ttgaacgaag aagaagattt aacataaatg
     7081 accgcattaa agaactaggt actttgattc ccaagtcaaa tgatccagac atgcgctgga
     7141 acaagggaac catcttaaaa gcatccgtgg actatatccg aaagttgcaa cgagaacagc
     7201 aacgcgcaaa agaacttgaa aaccgacaga agaaactgga gcacgccaac cggcatttgt
     7261 tgctcagaat acaggaactt gaaatgcagg ctcgagctca tggactttcc cttattccat
     7321 ccacgggtct ctgctctcca gatttggtga atcggatcat caagcaagaa cccgttcttg
     7381 agaactgcag ccaagacctc cttcagcatc atgcagacct aacctgtaca acaactctcg
     7441 atctcacgga tggcaccatc accttcaaca acaacctcgg aactgggact gaggccaacc
     7501 aagcctatag tgtccccaca aaaatgggat ccaaactgga agacatcctg atggacgaca
     7561 ccctttctcc cgtcggtgtc actgatccac tcctttcctc agtgtccccc ggagcttcca
     7621 aaacaagcag ccggaggagc agtatgagca tggaagagac ggagcacact tgttaggcgg
     7681 ccgcaatcga tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7741 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7801 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7861 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8041 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8161 tcaaaggacg gccgcacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     8221 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     8281 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     8341 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     8401 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     8461 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     8521 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
     8581 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttcacgcgtt
     8641 ttagcctggc agcatatcga gatcgaagtc atcgagagcg tcagcaggga gcatatccag
     8701 atcaaaatca tccagggcat cggcgggcag catgtccagg tcgaagtcgt ccagggcgtc
     8761 ggtgggtccg ccgctctcgc acttcagctg cttctcgagg ccgcagatga tcagttccag
     8821 gccgaacagg aaggcgggct cggcgccctg tctgtcgaac agctcgatgg cctgcttcag
     8881 cagggggggc atgctgtcgg tggtgggtgt ctctctttcc tctttggcga cctggtgttc
     8941 ctgttcttcc agcacgcagc ccagggtgaa gtggcccacg gcgctcaggg cgtacagggc
     9001 gttttccagg ctgaagccct gctggcacag gaaggccagc tggttttcca gtgtctcgta
     9061 ctgcttctcg gtgggtctgg tgcccaggtg cactttggcg ccgtcccggt ggctcagcag
     9121 ggcgcatctg tagctcttgg cgttgttccg caggaagtcc tgccagctct cgccttccag
     9181 agggcagctg tgggtgtggt gccggtccag catctcgatg ggcagggcgt ccagcagggc
     9241 ccgcttgttc ttcacgtgcc agtacagggt gggctgttcc acgcccagtt tctgggccag
     9301 cttccgggtg gtcaggccct cgatgcccac gccgttcagc agttccaggg cgctgttgat
     9361 cactttgctc ttgtccagcc ggctcatgtc gacattcttt gccaaaatga tgagacagca
     9421 caataaccag cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag
     9481 cagaggctct agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc
     9541 cccgaaggca gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc
     9601 ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt cctgccggcg ccgcaccgct
     9661 tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga
     9721 tttgggacaa aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc
     9781 ggctcgccgc gcctcgacag ccgccggcgc tccgggggcc gccgcgcccc tcccccgagc
     9841 cctccccggc ccgaggcggc cccgccccgc ccggcacccc cacctgccgc caccccccgc
     9901 ccggcacggc gagccccgcg ccacgccccg tacggagccc cgcacccgaa gccgggccgt
     9961 gctcagcaac tcggggaggg gggtgcaggg ggggttgcag cccgaccgac gcgcccacac
    10021 cccctgctca cccccccacg cacacacccc gcacgcagcc tttgttcccc tcgcagcccc
    10081 ccccgcaccg cggggcaccg cccccggccg cgctcccctc gcgcacactg cggagcgcac
    10141 aaagccccgc gccgcgcccg cagcgctcac agccgccggg cagcgcggag ccgcacgcgg
    10201 cgctccccac gcacacacac acgcacgcac cccccgagcc gctccccccg cacaaagggc
    10261 cctcccggag cccctcaagg ctttcacgca gccacagaaa agaaacaagc cgtcattaaa
    10321 ccaagcgcta attacagccc ggaggagaag ggccgtcccg cccgctcacc tgtgggagta
    10381 acgcggtcag tcagagccgg ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc
    10441 gaaggcagcg cgcagcgact cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc
    10501 gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc gctctgattg gctgccgccg
    10561 cacctctccg cctcgccccg cccccccccc cccccccccc cccccccccc ggcgcccgcc
    10621 cccccccccc ccccgccccc atcgctgcac aaaataatta aaaaataaat aaatacaaaa
    10681 ttgggggtgg ggaggggggg gagatgggga gagtgaagca gaacgtgggg ctcacctcga
    10741 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
    10801 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
    10861 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
    10921 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
    10981 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
    11041 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
    11101 aactagtcaa taatcaatgt cgagacccat agagcccacc gcatccccag catgcctgct
    11161 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
    11221 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
    11281 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
    11341 agaaggcaca gtcgaggctg atcagcgggt ttataacttc gtataatgta tgctatacga
    11401 agttatggta ccttactcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
    11461 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt
    11521 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc
    11581 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
    11641 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
    11701 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
    11761 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
    11821 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
    11881 cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag
    11941 ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
    12001 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
    12061 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc
    12121 gaccggtagc gctagcgtag gcgccggtca cagcttggat ctgtaacggc gcagaacaga
    12181 aaacgaaaca aagacgtaga gttgagcaag cagggtcagg caaagcgtgg agagccggct
    12241 gagtctaggt aggctccaag ggagcgccgg acaaaggccc ggtctcgacc tgagctttaa
    12301 acttacctag acggcggacg cagttcagga ggcaccacag gcgggaggcg gcagaacgcg
    12361 actcaaccgg cgtggatggc ggcctcaggt agggcggcgg gcgcgtgaag gagagatgcg
    12421 agcccctcga agcttcagct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac
    12481 ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta
    12541 cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc
    12601 gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc
    12661 accggagcga tcgcagatca tgcatccata gagcccaccg catccccagc atgcctgcta
    12721 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
    12781 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
    12841 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
    12901 gaaggcacag gtttaaacgg gccctctaga tcaggcaccg ggcttgcggg tcatgcacca
    12961 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
    13021 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
    13081 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
    13141 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
    13201 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
    13261 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc
    13321 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
    13381 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
    13441 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
    13501 gaggcgcacc gtgggcttgt actcggtcat ctcgagccta gggccgggat tctcctccac
    13561 gtcaccgcat gttagaagac ttcctctgcc ctctccgctg ccagatctct cgaggccctg
    13621 tgggaggaag agaagaggtc agaagcttat aacttcgtat aatgtatgct atacgaagtt
    13681 att
//
