LOCUS       sequence-232779-       10183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Mfsd10 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Mfsd10
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          213..1151
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1108..1128
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1167..1176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1176..1217
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2757..3306
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2901..2918)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3128..3147
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3309..3908
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3309..3328)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3805..3825
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3932..4520
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3985..4005)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4403..4414)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4727..5360
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5488..5504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5488..5504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5501..5523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5512..5528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5536..5542,5543..5560,5561..5566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5581..5602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5719..5736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5890..6478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5970..5989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6649..7440,7441..7509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7272..7291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7510..7614)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7662..7796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7699..7718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7753..7772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7964..8597
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8644..8769
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9266..9499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9684..9728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9991..10106
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
      601 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
      661 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
      721 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
      781 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
      841 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
      901 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
      961 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1021 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1081 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1141 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     1201 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gggctgggca ggggatgcgg
     1261 gctgtacgcc tcgtccgcct atacggcctc ggcccgcgtc cgagcgtcgc gtgatcatcg
     1321 tgctcttcct cggcctgcta ctcgacctcc tggcgttcac gctgttgctg cctctgctgc
     1381 ctgggctgct ggagagacac ggccgcgaac aagaccccct ctatggctcc tggcagcgtg
     1441 gggtagactg gtttgcttca gccatcggga tgccagcgga aaagaggtac aacagtgtcc
     1501 tgttcggagg tctcatcggc tcggccttct ccctcctgca gttcttctcc gcaccactca
     1561 caggagctgc ctctgattac ctggggaggc gcccagtgat gatgttgtcc ctgacaggtt
     1621 tggccatctc ctatgcagtg tgggctacct ctcggagctt taaggccttc ttggcctcca
     1681 gggtgatcgg aggcatcagc aaggggaacg ttaacctctc cacagccatt gtcgctgacc
     1741 tgggctcacc tccaacccgc agccaaggca tggcagtcat cggggtagcc ttctccttgg
     1801 cctttactct gggtcccatg ctgggagcat tcctgtctgt ggaaatggtg ccctggatca
     1861 gcctactttt tgccatctcc gacatgttgt tcatcttctg tttcttgcca gagacactgc
     1921 cccaggaaaa gcgggcatct tctgtcaccc tgggcttcca cactgctgcc cacctgctca
     1981 gccccctggc cctgcttcgc tttgcggctg tcactcacag tcaggacccg cctgctgagc
     2041 acagactcag aaacttacgc cgtttgggtc ttgtctactt cctctacctc ttcctgttct
     2101 caggcctgga gtacacactc agcttcctgg cacatcagcg cttccagttc agcagcctgc
     2161 agcagggcaa gatgtttttc ttcatcggcc tcaccatggc caccatacag ggcacctacg
     2221 cccggcggat cagccctggc aaggaagctg cagctgtgac gagggcaatg ctgctgctag
     2281 taccagcctt cctcttaatt ggctgggcac actcgctgcc tacgttgggc ttgggactga
     2341 tgctctactc ctttgctgct gctgttgtgg tgcctggtct gtccaccatg gtctccagtt
     2401 atggttctcc agggcaaaaa ggcacaatca tgggcatact gcggagccta ggcgccctag
     2461 gcagggcgtt ggggccggtg gtggctgcct cagtgtactg gctgactggg gcccaggtct
     2521 gcttcactgt gtgctcggcc ctctttctgc tccccttcct actcctgtgg aagctgaagc
     2581 atccagcaga gacttccaag gaggagtaat gggccgcgac gatgtttaaa cttcgaaacg
     2641 cgtgaattcc tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact
     2701 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     2761 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     2821 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     2881 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     2941 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3001 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     3061 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     3121 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     3181 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     3241 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     3301 ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     3361 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     3421 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     3481 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     3541 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     3601 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     3661 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     3721 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     3781 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     3841 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     3901 gtgcctgacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     3961 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4021 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4081 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4141 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4201 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4261 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4321 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4381 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4441 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4501 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     4561 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4621 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4681 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     4741 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4801 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     4861 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     4921 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     4981 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5041 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5101 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5161 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5221 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5281 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5341 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     5401 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     5461 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5521 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5581 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5641 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5701 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5761 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5821 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5881 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5941 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6001 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6061 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6121 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6181 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6241 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6301 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6361 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6421 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6481 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6541 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6601 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6661 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6721 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6781 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6841 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6901 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6961 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7021 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7081 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7141 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7201 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7261 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7321 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7381 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7441 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7501 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7561 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7621 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
     7681 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7741 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     7801 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     7861 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     7921 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
     7981 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     8041 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     8101 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     8161 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     8221 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     8281 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     8341 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8401 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8461 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8521 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8581 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8641 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8701 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8761 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8821 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8881 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8941 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9001 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9061 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     9121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     9961 gattagtgaa cggatctcga cggtatcgcc tttaaaagaa aaggggggat tggggggtac
    10021 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    10081 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
    10141 tatcgatgag gccctttcgt cttcactcga ggtgcccgtc agt
//
