LOCUS       sequence-232793-       10348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Lem2 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Lem2
FEATURES             Location/Qualifiers
     source          1..10348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          213..1151
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1108..1128
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1167..1176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1176..1217
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2922..3471
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3066..3083)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3293..3312
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3474..4073
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3474..3493)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3970..3990
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4097..4685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4150..4170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4568..4579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4892..5525
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5653..5669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5653..5669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5666..5688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5677..5693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5701..5707,5708..5725,5726..5731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5746..5767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5884..5901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6055..6643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6135..6154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6814..7605,7606..7674))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7437..7456
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7675..7779)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7827..7961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7864..7883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7918..7937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8129..8762
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8809..8934
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9431..9664
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9849..9893
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10156..10271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
      601 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
      661 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
      721 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
      781 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
      841 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
      901 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
      961 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1021 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1081 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1141 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     1201 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggccggcctg tcggacctgg
     1261 agttgcggcg agagctgcag gccctgggct tccagccagg ccccatcacc gacaccacgc
     1321 ggaacgtcta ccgcaacaag ctgcgccgcc tgcggggcga ggcccggctg cgcgacgacg
     1381 agcggctgcg ggaggacgcc gggccgcggg aggacgccgg gccgcggggc cccgagcggc
     1441 agcgggagga ggcccggcta cgcgaggaag cgccgctgcg cgcgcggccc gccgccagcg
     1501 tcctgcgctc ggagccctgg ccgctgtcgc cttccccgcc ggcgcctagc gcggcctccg
     1561 acgcctcggg gccgtacggc aacttcgggg cctccgcctc tccctgggcc gcgagccgcg
     1621 gcctctccta cccgccccac gccgggcccg ggccgctgcg acgccgcgcc tcggtccggg
     1681 gcagctcgga ggatgacgag gatacacgaa cgccggacag gcacgccccg ggccggggcc
     1741 gccactggtg ggccccgccg tcggcctctg cgcggccgca ctcggcgctc ctcggcgccg
     1801 acgctcgccc cggcctgaag ggctcgcgca ccggctcggc gggcgccggg cggacccgac
     1861 ccgaggtggg ccggtggctg gagcgctgcc tgtctcggct cctgctctgg gccagcctgg
     1921 ggctgctgct cggcttcttg gccatcctgt gggtgaagat gggcaagccc tcggcgccgc
     1981 aggaggcgga ggacaacatg aaattgttgc cggtcgactg tgagagaaaa acagatgagt
     2041 tctgccaggc caagcagaag gcggccctgt tggagctgct gcacgaactg tacaacttcc
     2101 tggccatcca ggcaggtaat tttgaatgtg gcaacccgga gaagctgaag agcaagtgca
     2161 tcccagtcct ggaggcgcag gagtacatag ctaatgtgac cagcagtccc tcttcgaggt
     2221 ttaaggctgc gctgacctgg atcctgagca gcaacaagga cgtgggcatc tggctgaaag
     2281 gggaagaccc atctgagctg gcgactacag tggacaaagt ggtctgcctg gagtccgccc
     2341 ggccccggat gggcataggc tgccgcctca gccgcgccct gctcaccgct gtcacccacg
     2401 tgctcatctt cttctggtgc ctggccttcc tgtgggggct gctgatcctc ctgaagtacc
     2461 gctggcggaa gctggaggag gaggagcagg ccatgtatga gatggtgaag aagatcatag
     2521 atgtggttca ggaccactat gtggactggg aacaggacat ggagcgctac ccctacgtgg
     2581 gcatcctcca cgtgcgggac agtctaatcc ccccacagag ccggcggcgc atgaagcgag
     2641 tatgggaccg tgctgtggaa ttcctggctt ccaatgaatc tcggatccag actgagtccc
     2701 accgagtggc cggggaggac atgctggtgt ggagatggac taagccttcc tctttctctg
     2761 attcagagcg ataatgggcc gcgacgatgt ttaaacttcg aaacgcgtga attcctcgag
     2821 ggcggccgct ctagagtcga cgggccgcgg taacaattgt taactaactt aagctagcaa
     2881 cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg gccgaagccg
     2941 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3001 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3061 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3121 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3181 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3241 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3301 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3361 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3421 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat accatgaccg
     3481 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     3541 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     3601 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     3661 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     3721 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     3781 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     3841 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     3901 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     3961 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     4021 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacccgggc
     4081 ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4141 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4201 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4261 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4321 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4381 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4441 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     4501 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4561 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4621 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4681 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     4741 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     4801 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     4861 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     4921 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     4981 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     5041 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     5101 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     5161 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     5221 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     5281 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     5341 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5401 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5461 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5521 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     5581 atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt
     5641 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5701 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5761 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6481 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6721 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6781 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6841 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6901 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6961 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7021 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7081 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7141 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7201 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7261 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7321 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7381 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7441 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7501 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7561 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7621 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7681 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7741 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7801 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     7861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7921 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     7981 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     8041 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     8101 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     8161 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     8221 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     8281 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     8341 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     8401 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     8461 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     8521 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     8581 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8641 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8701 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8761 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8821 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8881 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8941 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     9001 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     9061 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     9121 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     9181 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     9241 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     9301 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     9361 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     9421 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     9481 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     9541 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     9601 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     9661 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     9721 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     9781 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     9841 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     9901 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9961 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    10021 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
    10081 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
    10141 ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10201 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10261 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg atgaggccct
    10321 ttcgtcttca ctcgaggtgc ccgtcagt
//
