LOCUS       sequence-232794-        9592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Emerin in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Emerin
FEATURES             Location/Qualifiers
     source          1..9592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    596..721
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1218..1451
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1636..1680
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1943..2058
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2136..2347
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2348..3286
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3243..3263
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3302..3311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3311..3352
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4301..4850
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4445..4462)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4672..4691
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4853..5452
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4853..4872)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5349..5369
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5476..6064
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5529..5549)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5947..5958)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6271..6904
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7032..7048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7032..7048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7045..7067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7056..7072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7080..7086,7087..7104,7105..7110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7125..7146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7263..7280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7434..8022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7514..7533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8193..8984,8985..9053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8816..8835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9054..9158)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9206..9340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9243..9262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9297..9316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9508..9592,1..549)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
       61 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
      121 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
      181 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
      241 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
      301 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
      361 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      421 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      481 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      541 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      601 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      661 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      721 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
      781 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
      841 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
      901 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
      961 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1021 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     1081 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1141 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1201 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1261 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1321 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1381 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1441 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1501 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1561 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1621 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1681 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1741 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1801 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     1861 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     1921 aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt acagtgcagg
     1981 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2041 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttatcgatg
     2101 aggccctttc gtcttcactc gaggtgcccg tcagtgggca gagcgcacat cgcccacagt
     2161 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     2221 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     2281 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     2341 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     2401 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     2461 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     2521 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     2581 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     2641 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     2701 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     2761 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     2821 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     2881 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     2941 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     3001 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     3061 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     3121 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     3181 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     3241 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggatc
     3301 cgccgccatg ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggggggcgg
     3361 agggggtggg cccatggacg actatgcggt tttgtccgat actgagctgg ccgcagtgct
     3421 acgccagtac aacatccctc atgggcctat tgtgggttcc actcgcaagc tctacgaaaa
     3481 gaaaatcttc gagtacgaga cccagagaag gagactctta ccccccaact cgtcatcttc
     3541 ttcattctcc tatcagttct cagacttgga ttcagccgcc gtggactcag acatgtatga
     3601 tctgcccaaa aaagaagacg ccttacttta ccagagcaag gactataatg atgactacta
     3661 tgaggagagt tatttgacca ccaagacata cggggagcct gagtctgtgg gcatgtccaa
     3721 gagcttccgc cagccgggga cctcacttgt agatgctgat accttccatc accaggtgcg
     3781 tgatgacatt ttctcttctt tagaagagga aggcaaggat agggagcgcc tcatctatgg
     3841 ccaagacagt gcctaccaga gcatcgcaca ctaccgcccc atttccaatg tctccaggag
     3901 ctctctgggc ttatcatatt atcctacatc ctccacctct tctgtgtcct cctcttcatc
     3961 ttctccctct tcatggctta cccgacgtgc cattcggcca gagaagcagg cccctgctgc
     4021 tgctctgggc caggatcgac aggtcccact ctggggccag ctgttactct tcctggtctt
     4081 tgctgccttt ctgctctttg tttactattc catacaagct gaagaaggca accccttctg
     4141 gatggatccc taatgggccg cgacgatgtt taaacttcga aacgcgtgaa ttcctcgagg
     4201 gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac
     4261 ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc
     4321 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4381 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4441 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4501 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4561 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4621 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4681 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4741 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4801 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgaccga
     4861 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     4921 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     4981 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5041 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5101 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5161 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5221 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5281 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5341 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5401 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacccgggcg
     5461 gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5521 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5581 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5641 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5701 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5761 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5821 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5881 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5941 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6001 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6061 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     6121 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     6181 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6241 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     6301 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     6361 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     6421 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     6481 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     6541 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     6601 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     6661 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     6721 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6781 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6841 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6901 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6961 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     7021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8041 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8101 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9181 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     9241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9301 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     9361 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     9421 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     9481 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     9541 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag ca
//
