LOCUS       sequence-232808-        9304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Tmem128 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9304)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-EF1a-V5-Tmem128
FEATURES             Location/Qualifiers
     source          1..9304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        194..405
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          406..1344
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1301..1321
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1360..1369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1369..1410
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2071..2620
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2215..2232)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2442..2461
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2623..3222
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2623..2642)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3119..3139
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3246..3834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3299..3319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3717..3728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4041..4674
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4802..4818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4802..4818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4815..4837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4826..4842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4850..4856,4857..4874,4875..4880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4895..4916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5033..5050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5204..5792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5284..5303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5963..6754,6755..6823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6586..6605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6824..6928)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6976..7110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7013..7032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7067..7086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7278..7911
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7958..8083
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8580..8813
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8998..9042
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
       61 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      121 gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt cttcactcga
      181 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      241 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      301 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      361 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
      421 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
      481 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
      541 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
      601 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
      661 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
      721 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
      781 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
      841 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
      901 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
      961 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     1021 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     1081 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     1141 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     1201 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     1261 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     1321 caaagttttt ttcttccatt tcaggtgtcg tgaggatccg ccgccatggg taagcctatc
     1381 cctaaccctc tcctcggtct cgattctacg gggggcggag ggggtgggcc catggacgct
     1441 ccgtgggctc gggagcagct acggcggcgt tacctgtttc cttccgaagt agagctggac
     1501 tgcgaggacg aggccaagcc ggaaacaccc acagctgttg agaaaaagga gcgacctctt
     1561 ccaagactta acattcattc tggattctgg attttggcat ccatcgttgt gacctattat
     1621 gttgacttct ttaaaacctt taaagaaaat tttcacacca atagttggtt tctctttggc
     1681 ggagccttgt tatttgtcag cctgtcaatt gcattttact gcatagtcta tctggaatgg
     1741 taccgtggga ttgaagaata tgatgtcaaa tacccaacac tggtgcccat cacaactgca
     1801 actttcattg cggcagggat ttgcttcaac ctggctctgt ggaatgtgtg gtcatttttc
     1861 actcccttgc tgctgttcac ccagtttatg ggggtcgtga tgttcatctc actcctcgga
     1921 taatgggccg cgacgatgtt taaacttcga aacgcgtgaa ttcctcgagg gcggccgctc
     1981 tagagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc
     2041 tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2101 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     2161 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     2221 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     2281 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     2341 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     2401 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     2461 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     2521 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     2581 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgaccga gtacaagccc
     2641 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     2701 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     2761 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     2821 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     2881 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     2941 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3001 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3061 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3121 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3181 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacccgggcg gggcgcgtct
     3241 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3301 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3361 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3421 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3481 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3541 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3601 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3661 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3721 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3781 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     3841 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     3901 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     3961 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4021 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     4081 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     4141 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     4201 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     4261 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     4321 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     4381 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     4441 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     4501 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4561 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4621 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4681 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4741 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     4801 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4981 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5041 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5581 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5641 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5701 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5761 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5821 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6241 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6481 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6901 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     6961 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7081 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     7141 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     7201 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     7261 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     7321 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     7381 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     7441 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     7501 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     7561 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     7621 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     7681 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     7741 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7801 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7861 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7921 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7981 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8041 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8101 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8161 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8221 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8281 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8341 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8401 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     8461 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8521 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8581 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8641 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8701 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8761 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8821 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8881 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     8941 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9001 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     9061 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     9121 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     9181 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     9241 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     9301 cgcc
//
