LOCUS       sequence-232809-       10324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Vrk2 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10324)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 10324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Vrk2
FEATURES             Location/Qualifiers
     source          1..10324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          213..1151
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1108..1128
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1167..1176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1176..1217
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2898..3447
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3042..3059)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3269..3288
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3450..4049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3450..3469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3946..3966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4073..4661
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4126..4146)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4544..4555)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4868..5501
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5629..5645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5629..5645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5642..5664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5653..5669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5677..5683,5684..5701,5702..5707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5722..5743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5860..5877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6031..6619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6111..6130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6790..7581,7582..7650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7413..7432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7651..7755)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7803..7937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7840..7859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7894..7913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8105..8738
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8785..8910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9407..9640
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9825..9869
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10132..10247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
      601 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
      661 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
      721 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
      781 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
      841 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
      901 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
      961 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1021 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1081 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1141 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     1201 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggcaccaaga agaaaagaga
     1261 aatataagct tccggttcca ctcccagaag gcaaaattct ggatgatatg gaaggaaacc
     1321 ggtgggcact gggcaagatg atcggctctg gagggtttgg actgatatac ttagctttcc
     1381 ccacaaataa accaaacaaa gatgcaagac atgtcataaa gctggagtat caagaaaatg
     1441 gcccattatt ttcagagctt aaattttatc agagagctgc aaaaagagaa tgtatccaaa
     1501 agtggataca acagaggaaa cttgattatt taggaattcc tgtattttat ggatttggtc
     1561 tgactgattt caaaggaaga agttacagat tcatggtaat ggaaagactt ggaatagatt
     1621 tacagaaact cctggaccag aatggtggtt ttaaaaaatt aaccgtccta caacttggca
     1681 tcaggatgct ggatgtactg gaatatatac atgaaaatga gtatgttcat ggtgatataa
     1741 aagccgcaaa tctactgttg gattttacaa atccagaccg ggtttatctt gcagactatg
     1801 gactttccta cagatattgt cccaatggga accacaaaca gtatcaggaa gatcccagaa
     1861 agggccataa tgggacaata gagtttacaa gtttggatgc acacaaagga gtggccccgt
     1921 ccaggaggag tgatgttgaa atccttggtt actgcatgct gcactggctc ttcgggaagc
     1981 ttccttggga agcaaagctg gacgaccctg tggctgtcca gactgctaaa accaacctgc
     2041 tggatgaact cccagagtcg gtgcttaagt gggctccttc tggaagcagt tgcagtgaac
     2101 ttgtcaagta cttgatgtat gttcataatt tagcttatga tgacaagcca gactatcaga
     2161 agctcaagaa aattttgaat ccagatggag tacctttagg gccactggaa ttttccacta
     2221 aagtacagag tgtccatgtg cggactccag cccaacaaaa agttgattca ccaaaggcta
     2281 caagaaaacc agccaatgag ttcccagcta agttcccaaa gaaagtccac agggagacaa
     2341 gagcaaggca gagagaggag caggaagaca gccagcccac aatgctgcag agcaggccgg
     2401 cagcaccgga aaactcaagg acaaggaaaa tacatgaata ttctgatatt ttcagtgaga
     2461 tgcagagtct tcaacaaaca cccagctata tgagtttcca aggttcatat tgcaagccct
     2521 atctggactg cactagaaga gatcccatca ggaagccaag atccctgcct cggtacagac
     2581 acacgcccac gggtaatctg ggagtcacag acttggaaag ttctccaagg ttttggcctg
     2641 caatttttca gttgactctg agtgaagaga caaaggcaga tgtgtattac tatgggatca
     2701 ctatattctg tctgctgata tttgtatttc ttgctttgta ttttctctaa tgggccgcga
     2761 cgatgtttaa acttcgaaac gcgtgaattc ctcgagggcg gccgctctag agtcgacggg
     2821 ccgcggtaac aattgttaac taacttaagc tagcaacggt ttccctctag cgggatcaat
     2881 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     2941 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3001 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3061 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3121 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3181 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3241 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3301 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3361 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3421 ggttttcctt tgaaaaacac gataatacca tgaccgagta caagcccacg gtgcgcctcg
     3481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3661 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     3841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4021 ggtgcatgac ccgcaagccc ggtgcctgac ccgggcgggg cgcgtctgga acaatcaacc
     4081 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4141 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4201 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4261 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4321 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4381 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4441 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4501 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4561 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4621 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     4681 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     4741 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     4801 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4861 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4921 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     4981 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     5041 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     5101 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     5161 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     5221 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     5281 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     5341 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5401 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5461 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5521 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     5581 ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5641 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5701 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5761 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5821 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5881 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6661 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6721 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6781 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6841 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6901 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6961 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7021 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7081 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7141 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7201 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7261 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7321 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7381 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7441 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7501 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7561 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7621 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7681 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7741 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7801 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7921 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     7981 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     8041 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     8101 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     8161 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     8221 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     8281 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     8341 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     8401 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     8461 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     8521 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     8581 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8641 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8701 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8761 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8821 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8881 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8941 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9001 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9061 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9121 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9181 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9241 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     9301 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     9361 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     9421 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     9481 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     9541 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     9601 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     9661 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     9721 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     9781 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     9841 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     9901 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     9961 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    10021 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
    10081 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga
    10141 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    10201 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
    10261 agggacagca gagatccagt ttatcgatga ggccctttcg tcttcactcg aggtgcccgt
    10321 cagt
//
