LOCUS       sequence-232810-        9493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Arl6ip6 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Arl6ip6
FEATURES             Location/Qualifiers
     source          1..9493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          213..1151
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1108..1128
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1167..1176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1176..1217
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2067..2616
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2211..2228)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2438..2457
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2619..3218
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2619..2638)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3115..3135
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3242..3830
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3295..3315)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3713..3724)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4037..4670
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4798..4814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4798..4814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4811..4833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4822..4838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4846..4852,4853..4870,4871..4876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4891..4912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5029..5046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5200..5788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5280..5299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5959..6750,6751..6819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6582..6601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6820..6924)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6972..7106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7009..7028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7063..7082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7274..7907
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7954..8079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8576..8809
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8994..9038
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9301..9416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
      601 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
      661 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
      721 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
      781 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
      841 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
      901 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
      961 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1021 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1081 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1141 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     1201 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gtcgttcgtg gagagttgga
     1261 ggtttgcggg cgcgcggagg cgccgtcagg ttaccccggg ccctgcgact cggcccgggt
     1321 attccgacta cactcagggg gacagctggg gtgagggcga aggcgacgaa gacgagggat
     1381 gcgaccaagt ggcccgcgac ttgcgggcag agttctcagc cagggcgtcg tcggagacca
     1441 agagggcccc gctactcccc cgggtcgggg acgggtctcc ggtcctgccg gataagcgca
     1501 acggcatctt cccggccact gcggccaagc gaacccaggc tcggcggtgg cccatccaag
     1561 ccctttccat tctctgttcg ctgctgttcg ccgtcctcct cgctttcctt ctcgccatcg
     1621 cctacatgat cgttaaagaa ttgcatgcag aaaacttgaa aaatgaagat gatatacata
     1681 ctgggctgtt aggcttctgg tctctgctga taatatctct aactgctgga ttgtcctgtt
     1741 gcagcttttc ttggacagtg acttactttg attcttttga accaggaatg tttccaccca
     1801 ctcctctttc acctgctagg ttcaagaaac tgactggaca ttctttccac atgggctaca
     1861 gcatggcaat tttgaatggt gttgtcgctg ctctcactgt ggcatggtgc ctcatgtaat
     1921 gggccgcgac gatgtttaaa cttcgaaacg cgtgaattcc tcgagggcgg ccgctctaga
     1981 gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt tccctctagc
     2041 gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     2101 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     2161 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     2221 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     2281 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     2341 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     2401 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     2461 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     2521 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     2581 cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac aagcccacgg
     2641 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     2701 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     2761 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2821 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2881 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2941 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     3001 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     3061 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     3121 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     3181 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc cgggcggggc gcgtctggaa
     3241 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3301 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3361 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3421 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3481 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3541 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3601 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     3661 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3721 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3781 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     3841 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     3901 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     3961 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4021 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     4081 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     4141 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     4201 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     4261 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     4321 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     4381 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     4441 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     4501 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4561 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4621 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     4681 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4741 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4981 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5641 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     6961 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7081 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     7141 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     7201 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     7261 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     7321 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7381 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7441 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7501 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7561 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7621 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7681 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7741 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7801 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7861 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7921 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7981 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8041 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8101 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8161 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8221 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8281 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8341 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8401 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     8461 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8521 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8581 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8641 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8701 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8761 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8821 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8881 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8941 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9001 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9061 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9121 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9181 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9241 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     9301 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     9361 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     9421 gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt cttcactcga
     9481 ggtgcccgtc agt
//
