LOCUS       sequence-232811-       11287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Smpd4 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Smpd4
FEATURES             Location/Qualifiers
     source          1..11287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        78..289
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          290..1228
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1185..1205
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1244..1253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1253..1294
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3938..4487
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4082..4099)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4309..4328
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4490..5089
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4490..4509)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4986..5006
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5113..5701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5166..5186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5584..5595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5908..6541
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6669..6685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6669..6685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6682..6704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6693..6709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6717..6723,6724..6741,6742..6747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6762..6783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6900..6917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7071..7659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7151..7170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7830..8621,8622..8690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8453..8472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8691..8795)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8843..8977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8880..8899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8934..8953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9145..9778
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9825..9950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10447..10680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10865..10909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11172..11287
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tcgggtttat tacagggaca gcagagatcc agtttatcga tgaggccctt tcgtcttcac
       61 tcgaggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      121 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      181 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      241 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      301 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      361 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      421 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      481 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
      541 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
      601 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
      661 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
      721 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
      781 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
      841 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
      901 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
      961 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     1021 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1081 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1141 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1201 ggttcaaagt ttttttcttc catttcaggt gtcgtgagga tccgccgcca tgggtaagcc
     1261 tatccctaac cctctcctcg gtctcgattc tacggggggc ggagggggtg ggcccatggc
     1321 gttccctcac ctacaacagc ctagtttcct tctggctagc ctgaaagctg actctataaa
     1381 taagcccttt gcacaacgtt gccaagattt ggtgaaagtc attgaggatt ttccagccaa
     1441 ggaattgcat gccgtcttcc catggctggt ggaaagcatc tttggcagcc tggatggtgt
     1501 ccttgttggt tggaatctcc gatgcctaca gggacgtgtg aaccctgtgg agtacagcac
     1561 agcgatggaa tttctagacc caagtggtcc aatgatgaag ttggtttata aacttcaagc
     1621 tgaagactat aactttgatt ttcctgtctc ctgtcttcct ggccctgtga aggcatccat
     1681 tcaagagaat gtcctccctg acagtcctct gtaccacaac aaagtccagt ttcccccgac
     1741 cggaggcctt ggcctgaacc tggccctcaa tccatttgaa tactatatgt tctactttgc
     1801 tttgagcctc atctctcaaa agccaatgtc tatgaccctc catgtccgta cttcggactg
     1861 tgcctatttc accctggtgg ataggtacct atcatggttc ctacccactg aaggcagcgt
     1921 acctccacca ctctgctcca gtccaggagg ctccagcccc tcaccagctc ccagaacacc
     1981 agccatgccc tttgcctcct atggcctcca cactagcctc ttgaagcgac acatctctca
     2041 tcagacatct gtgaatgcag accctgcatc ccatgagatc tggcggtcag aaactctcct
     2101 ccaggttttt gtggaaatgt ggcttcatca ttactccctg gagatgtacc aaaaaatgca
     2161 gtcccctcat gccaagttgg aggttctaca ctaccgactc actgtctcca gcgccctcca
     2221 cagccctgcc caacccagcc tccaggccct ccacgcctac caagagtcct tcacgcctac
     2281 tgaagagcac gtgctggtgg tacgcctgct gctgaagcac ctgcatgcct ttgccaacag
     2341 cctgaagcca gatcaggctt cgccatctgc acactcccat gccaccagtc cgctggagga
     2401 attcaaacgg gccgcagtgc ctcgctttgt ccagcagaag ctgtacgtct tcctgcagca
     2461 ctgttttggc cactggcctc tggatgcaac cttcagagct gtcttggaga tgtggctgag
     2521 ctacctgcag ccatggaggt atgcccctga gaaacaggct cagggcagcg atccccagcc
     2581 acggtgtgtg tcagagaagt gggcaccctt catccaggaa aacctgctga tgtacaccaa
     2641 gctgtttgtg agctttctga accgtgccct acgcacagac cttgtcagcc ccaagaatgc
     2701 actcatggtc ttccgagtgg ctaaggtctt tgcccagccc aacctggctg agatgatcca
     2761 gaaaggtgag cagctgttcc tggagccgga gctcatcatc ccccaccgcc aacaccggct
     2821 cttcacagtc accaccagct tcctgtcacc atggccccct gttgtgacag atgcctcctt
     2881 caaggtgaag agccatgtct atagcctaga gggccaagac tgcaaatata ccccaatgtt
     2941 tggtcctgaa atccgaaccc tggtcttgcg ccttgctcag ctcatcacac aggccaagca
     3001 gactgccaag tccatctctg atcagtatgt ggaaagccca actggccgct ccttcctgtc
     3061 atggctgacc tttggcctca cagacacaaa tagctgctac ccagccaatg acctggatga
     3121 aataggtcag gacagcatcc gcaagacaga cgagtactta gaaaaggccc tggagtacct
     3181 gcgccagata ttccggctca gcgaagccca gctggcccag ctcacgcttg ccctggggag
     3241 tgctcgggac gagaatggga agcagcagct cccagattgc attgtggggg aagaaggact
     3301 catccttaca cccttgggcc gctaccagat cattaatggt ctgcgaaggt tcgagattga
     3361 gtaccaggga gacttagagc tgcagcccat ccggagctac gaaatcacca gtctggtccg
     3421 tgcactcttc cggctgtcct ctgccatcaa ccgtagattt gcaggccaga tggcagctct
     3481 gtgctcccgg aatgacttcc ttggcagctt ctgtcgctac cacctcactg agcctgcctt
     3541 gagcaacaga catctgctga gccctgtagg gcggaggcag gtcaccaatc ctgcacgggg
     3601 ccccaggctc agcctccgtt tcctgggcag ttaccggaca ctgctcctgc tcctgatggc
     3661 cttctttgtg gcttctctgt tctgcattgg gcccctgtcc tgctccttgc tcctggtcct
     3721 aggatacgtc ctctatgcca tagctatgac actgctcact gaacggggga agctgcacca
     3781 gctctgataa tgggccgcga cgatgtttaa acttcgaaac gcgtgaattc ctcgagggcg
     3841 gccgctctag agtcgacggg ccgcggtaac aattgttaac taacttaagc tagcaacggt
     3901 ttccctctag cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     3961 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4021 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4081 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4141 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4201 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4261 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4321 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4381 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4441 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tgaccgagta
     4501 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4561 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4621 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4681 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4741 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4801 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4861 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4921 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4981 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5041 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac ccgggcgggg
     5101 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     5521 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5701 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     5761 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     5821 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     5881 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     5941 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     6001 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6061 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     6121 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     6181 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     6241 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     6301 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     6361 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6421 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6481 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6541 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6601 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
     6661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7501 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8821 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     8881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8941 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9001 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     9061 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9121 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     9181 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     9241 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     9301 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     9361 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     9421 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     9481 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     9541 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     9601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     9841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     9901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     9961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    10021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    10081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    10141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    10201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    10261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
    10321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    10381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    10441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    10501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    10561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    10621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    10681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    10741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    10801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    10861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    10921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    10981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    11041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
    11101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
    11161 acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11221 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11281 aaaattt
//
