LOCUS       sequence-232812-        9824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Tmx4 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 9824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-Tmx4-V5
FEATURES             Location/Qualifiers
     source          1..9824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        194..405
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          406..1344
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1301..1321
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2413..2454
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2591..3140
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2735..2752)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2962..2981
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3143..3742
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3143..3162)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3639..3659
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3766..4354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3819..3839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4237..4248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4561..5194
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5322..5338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5322..5338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5335..5357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5346..5362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5370..5376,5377..5394,5395..5400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5415..5436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5553..5570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5724..6312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5804..5823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6483..7274,7275..7343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7106..7125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7344..7448)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7496..7630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7533..7552)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7587..7606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7798..8431
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8478..8603
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9100..9333
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9518..9562
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
       61 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      121 gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt cttcactcga
      181 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      241 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      301 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      361 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
      421 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
      481 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
      541 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
      601 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
      661 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
      721 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
      781 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
      841 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
      901 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
      961 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     1021 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     1081 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     1141 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     1201 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     1261 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     1321 caaagttttt ttcttccatt tcaggtgtcg tgaggatccg ccatcggccc gcccgccgcc
     1381 atgacaggcg gcttctgcgt cccggtactc ctggccgcct ggctcgcagc ggcagcggcc
     1441 gagggtctcg agcaggccgc gctgccggcc gaggagagcc gtgtgcagcc catgaccgcc
     1501 tccaactgga cgctggtgat ggagggcgag tggatgctga aattttatgc tccatggtgt
     1561 ccatcctgcc agcagactga ttctgaatgg gagacttttg caaagaatgg agaaacactt
     1621 cagatcagtg tgggaaaggt ggatgtcatt caagagccag gtttgagtgg ccgcttcttt
     1681 gtcaccactc tcccagcatt ttttcatgca aaagatggga tattccgccg ttaccgtggt
     1741 ccaggaatct atgaagattt acagaattac atattagaga agaaatggca atcagttgag
     1801 cctctcactg gctggaagtc cccagcctct ctaacgatgt ctggaatggc tggtcttttt
     1861 agcatctctg gcaagatttg gcaccttcac aactatttca cagtgaccct tggaattcct
     1921 gcatggtgtt cttacgtttt tttcgtcatt gccacattgg tgtttggcct gttcatgggt
     1981 ctgatcctgg tggtgatttc agaatgtttc tgtgtgcctc ttccaagagc ttcctctgaa
     2041 cgctgtgagc aggagcagag tactggagag gctcaaggag ctgagcagct acaggatgca
     2101 gaggaagaaa aggatgactc aaatgaagaa gaaaacaaag acagtcttgt ggatgatgag
     2161 gaagagaaag aggacattgg agatgaggat gaaggagaag aagatgagga agaggacaac
     2221 ttggcaggca tcatggctga ggagagaagt gacaccaacg agcgagcagt tgtgaaggag
     2281 ggtagtgtgt ccccaaagga ggacggagct cacccagctg acactcagga cgtggtggaa
     2341 gacgccctga ggcagcgcaa gagtcagaat gccaacaagg gatctggtgg gccgggcggg
     2401 ggtggcgggg gaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtgagtt
     2461 taaacttcga aacgcgtgaa ttcctcgagg gcggccgctc tagagtcgac gggccgcggt
     2521 aacaattgtt aactaactta agctagcaac ggtttccctc tagcgggatc aattccgccc
     2581 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2641 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2701 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     2761 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     2821 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     2881 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     2941 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3001 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3061 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3121 ctttgaaaaa cacgataata ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3181 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3241 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3301 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3361 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3421 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3481 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3541 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3601 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3661 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3721 gacccgcaag cccggtgcct gacccgggcg gggcgcgtct ggaacaatca acctctggat
     3781 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3841 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3901 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3961 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4021 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4081 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4141 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4201 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4261 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4321 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     4381 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4441 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4501 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     4561 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     4621 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     4681 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     4741 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     4801 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     4861 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     4921 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     4981 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     5041 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5101 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5161 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     5221 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     5281 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5341 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5401 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5461 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7441 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     7501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7621 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     7681 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     7741 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     7801 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     7861 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7921 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7981 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8041 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8101 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8161 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8221 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8281 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8341 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8401 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8461 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8521 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8581 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8641 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8701 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8761 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8821 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8881 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8941 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9001 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9061 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9121 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9181 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9241 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9301 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9361 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9421 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9481 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9541 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9601 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9661 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9721 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     9781 agacagatcc attcgattag tgaacggatc tcgacggtat cgcc
//
