LOCUS       sequence-232826-        2054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for expression of AcrIIC1 (no miR binding sites, control 
            vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri
            J, Mastel M, Borner K, Eils R, Grimm D, Niopek D
  TITLE     Cell-specific CRISPR-Cas9 activation by microRNA-dependent 
            expression of anti-CRISPR proteins.
  JOURNAL   Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 
            10.1093/nar/gkz271.
  PUBMED    30982889
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271."
COMMENT     Sequence Label: AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)
FEATURES             Location/Qualifiers
     source          1..2054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        246..494
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     246..266
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     417..436
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        615..994
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        995..1198
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1149..1169
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1243..1262
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1243..1261
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1664..1681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1670..1894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atctgatatc ggcgcgcccg agtccaacac ccgtgggaat cccatgggca ccatggcccc
       61 tcgctccaaa aatgctttcg cgtcgcgcag acactgctcg gtagtttcgg ggatcagcgt
      121 ttgagtaaga gcccgcgtct gaaccctccg cgccgccccg gccccagtgg aaagacgcgc
      181 aggcaaaacg caccacgtga cggagcgtga ccgcgcgccg agcgcgcgcc aaggtcgggc
      241 aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      301 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      361 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      421 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      481 aaggacgaaa caccgagtct tcgaggaaga caggtttaag agctatgctg gaaacagcat
      541 agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      601 ttttttgcgg ccgcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      661 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      721 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      781 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      841 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      901 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      961 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     1021 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1081 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1141 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     1201 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
     1261 ggagacccaa gctggctagc gccaccatgg caaataaaac gtacaagata ggcaaaaacg
     1321 ctgggtacga tgggtgtggt ctgtgcctcg ctgcgatcag cgagaatgag gctatcaagg
     1381 tcaaatactt gcgcgacatc tgcccggact acgatggcga cgacaaagct gaagattggt
     1441 tgaggtgggg gacggattct cgcgttaagg ccgctgcgct tgaaatggag caatatgcct
     1501 acacatccgt cggtatggcg tcctgttggg agttcgttga gttgtgactc gagcggccgc
     1561 cactgtgctg gatatctgca gaattagaga cgccgttgat gttgaagttt atataccgtt
     1621 gatgttgaag tcgtctctag cttaagttta aaccgctgat cagcctcgac tgtgccttct
     1681 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     1741 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     1801 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     1861 agcaggcatg ctggggatgc ggtgggctct atgggtcgac gatatcagat ctaggaaccc
     1921 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgcccgg
     1981 gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc
     2041 agagagggag tggc
//
