LOCUS       sequence-232839-       10327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-05-29"; volume: "19(1):59. doi"; pages: " 
            10.1186/s13059-018-1443-z"
COMMENT     Sequence Label: PABE-6
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1975..1998,1999..2001,2002..6075)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5988..5996
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6088..6108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6121..6141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6199..6375
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(6929..6945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6929..6945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6942..6964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6953..6969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6977..6983,6984..7001,7002..7007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7022..7043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7160..7177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7331..7919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7411..7430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8090..8881,8882..8950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8713..8732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8951..9055)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt
       61 atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt
      121 ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttaattctgt
      181 ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca
      241 tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat
      301 gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg
      361 tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct
      421 ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt
      481 taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat
      541 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct
      601 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca
      661 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg
      721 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aaagcttatg
      781 tccgaggtgg agttctctca cgagtattgg atgaggcacg ctcttacact tgctaagaga
      841 gcttgggacg aaagagaagt gccagttggc gccgttcttg tgcataataa tagggtgatc
      901 ggcgagggtt ggaatagacc aattggaagg catgatccaa cagctcacgc agagattatg
      961 gctctcagac aaggcggcct cgttatgcag aactacaggc tcattgacgc tacactctac
     1021 gtgacactcg aaccttgcgt tatgtgcgcc ggagctatga ttcattctag gattggcagg
     1081 gtcgtgtttg gagctaggga cgctaaaaca ggagccgccg gatctcttat ggacgtgttg
     1141 catcatccag gcatgaacca tagggtggag attacagagg gcattcttgc agacgagtgc
     1201 gctgctcttc tttccgattt cttcaggatg agaaggcagg agattaaggc ccagaagaag
     1261 gctcagtctt ctacagatag cggaggatct tccggaggat ctagcggctc cgagacacca
     1321 ggaacatccg aaagcgctac accagaatct agcggaggct cttccggagg atcttctgaa
     1381 gtggagttct cccacgagta ttggatgagg cacgctctta cacttgctaa aagggctagg
     1441 gacgaaaggg aagttccagt tggagctgtt ctcgtgctca ataacagggt gattggcgag
     1501 ggttggaata gagccattgg actccatgat ccaacagctc acgcagagat tatggctctt
     1561 agacaaggcg gcctcgttat gcagaattac agactcatcg acgccacact ctacgttacc
     1621 ttcgaacctt gcgttatgtg cgccggagct atgatccatt ctaggattgg cagggtcgtg
     1681 ttcggcgtta gaaacgctaa gacaggagct gcaggctctc ttatggacgt tcttcattac
     1741 ccaggcatga atcatagagt ggagatcaca gaaggcattc ttgcagacga gtgcgcagct
     1801 ctcctttgct atttcttcag gatgccgagg caagttttca acgctcagaa gaaggcccag
     1861 tcttctacag attccggcgg atcttctgga ggatctagcg gctccgagac accaggaaca
     1921 tccgaatccg ctacaccaga gtcttctgga ggatctagcg gaggatctct taaggacaag
     1981 aagtactcga tcggcctcgc cattgggact aactctgttg gctgggccgt gatcaccgac
     2041 gagtacaagg tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc
     2101 aagaagaatc tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg
     2161 ctcaagcgca ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag
     2221 gagattttct ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag
     2281 tcattcctcg tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc
     2341 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
     2401 gactccacag ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag
     2461 ttcaggggcc atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag
     2521 ctgttcatcc agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg
     2581 tcaggcgtcg acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag
     2641 aacctgatcg cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg
     2701 ctcagcctgg ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag
     2761 ctgcagctct ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc
     2821 gatcagtacg cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct
     2881 gatattctca gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag
     2941 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc
     3001 cccgagaagt acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt
     3061 gacggcgggg cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg
     3121 gacggcacgg aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg
     3181 acattcgata acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg
     3241 aggaggcagg aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt
     3301 ctgactttca ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct
     3361 tggatgaccc gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac
     3421 aagggcgcta gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca
     3481 aatgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag
     3541 ctgactaagg tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag
     3601 cagaagaagg ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag
     3661 ctcaaggagg actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt
     3721 gaggaccgct tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat
     3781 aaggacttcc tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc
     3841 actctgttcg aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc
     3901 gatgacaagg tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc
     3961 aggaagctca tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg
     4021 aagagcgatg gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca
     4081 ttcaaggagg atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat
     4141 atcgcgaacc tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt
     4201 gtggacgagc tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg
     4261 gcccgggaga atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg
     4321 atcgaggagg gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac
     4381 acgcagctgc agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat
     4441 gtggaccagg agctggatat taacaggctc agcgattacg acgtcgatca tatcgttcca
     4501 cagtcattcc tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac
     4561 cggggcaagt ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg
     4621 cgccagctcc tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct
     4681 gagaggggcg ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag
     4741 acacggcaga tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac
     4801 gatgagaatg acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc
     4861 tccgacttca ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat
     4921 gcccatgacg cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag
     4981 ctcgagagcg agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc
     5041 aagtcggagc aggagattgg caaggctacc gccaagtact tcttctactc taacattatg
     5101 aatttcttca agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc
     5161 gagacgaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
     5221 aggaaggttc tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc
     5281 gggttctcta aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag
     5341 aaggactggg atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc
     5401 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg
     5461 ctggggatca cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag
     5521 gcgaagggct acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc
     5581 ttcgagctgg agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg
     5641 aacgagctgg ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag
     5701 aagctcaagg gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag
     5761 cattacctcg acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc
     5821 gacgcgaatc tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg
     5881 gagcaggctg agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct
     5941 ttcaagtact tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg
     6001 gacgcgaccc tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct
     6061 cagctcgggg gcgactccgg cggcagccca aagaagaagc ggaaggtgtc tggaggttct
     6121 cctaagaaaa agagaaaagt gtaggaattc ggtacgctga aatcaccagt ctctctctac
     6181 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa
     6241 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     6301 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     6361 actaaaatcc agatctccta aagtccctat agatctttgt cgtgaatata aaccagacac
     6421 gagacgacta aacctggagc ccagacgccg ttcgaagcta gaagtaccgc ttaggcagga
     6481 ggccgttagg gaaaagatgc taaggcaggg ttggttacgt tgactccccc gtaggtttgg
     6541 tttaaatatg atgaagtgga cggaaggaag gaggaagaca aggaaggata aggttgcagg
     6601 ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac gctactatac ttatactata
     6661 cgctaaggga atgcttgtat ttatacccta taccccctaa taacccctta tcaatttaag
     6721 aaataatccg cataagcccc cgcttaaaaa ttggtatcag agccatgaat aggtctatga
     6781 ccaaaactca agaggataaa acctcaccaa aatacgaaag agttcttaac tctaaagata
     6841 aaagatcttt caagatcaaa actagttccc tcacaccggt gacggggatc gcatgcgata
     6901 tctcgagatc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9061 ttccccgaaa agtgccacct gccagtgcca agctaattcg agctcggtac ctgacccggt
     9121 cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata
     9181 ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact
     9241 ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat
     9301 ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta
     9361 cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat
     9421 ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat
     9481 agactaattt ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta
     9541 aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg
     9601 actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg
     9661 tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca
     9721 gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg
     9781 cctctggacc cctctcgatc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc
     9841 atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct
     9901 cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt
     9961 cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg
    10021 ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct
    10081 tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc
    10141 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt
    10201 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt
    10261 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc
    10321 gcagacg
//
