LOCUS       sequence-232865-        5196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 
            origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Liu Y, Landick R, Raman S
  TITLE     A Regulatory NADH/NAD+ Redox Biosensor for Bacteria.
  JOURNAL   ACS Synth Biol. 2019 Feb 15;8(2):264-273. doi: 
            10.1021/acssynbio.8b00485. Epub 2019 Jan 23.
  PUBMED    30633862
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00485"; journalName: "ACS Synth Biol"; date: 
            "2019-02-15- 15"; volume: "8"; issue: "2"; pages: "264-273"
COMMENT     Sequence Label: pB-Rex
FEATURES             Location/Qualifiers
     source          1..5196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(942..961)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(943..961)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(971..988)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(971..987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(980..1002)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(1694..2356)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2357..3126
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4409..4942)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5131..5136,5137..5153,5154..5159))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgtatgc atgagctcta ctacgtcagt gtcgtgccat agatctcata agggagagcg
       61 tcgagatccc gggcgcgcct tgacaattaa tcatccggct cctaggatgt gtggagggac
      121 gactaacacc agacgattct attacaaatc cagaatgaat aaggatcaat caaaaattcc
      181 gcaggcgacg gcgaaacggc tgccgcttta ctatcgcttt ttaaagaatc tgcatgcgtc
      241 aggaaaacag cgtgtatcat ccgctgaact cagtgatgcc gtaaaggttg attctgccac
      301 gattcggagg gatttttcct attttggagc tcttggcaaa aaaggatacg gatataatgt
      361 ggattatttg ctgtcttttt tccggaaaac gcttgatcag gatgagatga cagacgtcat
      421 cttgattggt gtcgggaact tgggaacggc atttcttcac tataatttca caaaaaacaa
      481 taacacaaaa atttctatgg cttttgatat aaatgagagt aaaataggaa ctgaggtagg
      541 cggcgtgccc gtctataacc ttgatgacct tgaacaacac gtaaaagatg aatcagttgc
      601 cattcttaca gtgccagcag ttgccgctca atccattaca gacagattgg tcgcattagg
      661 aatcaaggga atccttaatt ttacgccggc ccgtttgaat gtgccggaac acattcgaat
      721 tcatcatata gatttagcag ttgagcttca gtcactggtt tattttttga agcattattc
      781 agttttagag gaaatcgaat gagcttgata tcgaattcct gcagcccggg ggatccctag
      841 ttctagaaat aagttaaata aagccctgag tttaaccgct cggggctttt tgcgttttaa
      901 gcacactaga gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt
      961 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1021 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1081 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     1141 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     1201 ggccgactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1261 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1321 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
     1381 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
     1441 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
     1501 attctgcctc ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc
     1561 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     1621 gtatagggcg gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg
     1681 cgcaacccaa gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc
     1741 catcgtccag aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc
     1801 attcctccgt ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc
     1861 tgggcggctc ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga
     1921 tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca
     1981 cggcggcact gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt
     2041 cattgaccac gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc
     2101 gctcggccac caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct
     2161 tggaaagtgt cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt
     2221 gatgcagcag cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg
     2281 ctttgcgttc cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc
     2341 tggactgcgt ggccatgctt atctccatgc ggtagggtgc cgcacggttg cggcaccatg
     2401 cgcaatcagc tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca
     2461 gtcaattaca gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag
     2521 ctgttgcgta cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat
     2581 atctagttct ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg
     2641 cggctccccc tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt
     2701 cggccttgcc caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc
     2761 ggggggcagg cgggcgggct tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt
     2821 ccaggcgcgt caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca
     2881 cttggtgtcc aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag
     2941 aaaattaaaa aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg
     3001 tggtcgaagg ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag
     3061 cctgtccatc agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt
     3121 ggccgctcgc ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc
     3181 agggcgaagc ccggagagca agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact
     3241 ttgcgaagca aagtctagtg agtatactca agcattgagt ggcccgccgg aggcaccgcc
     3301 ttgcgctgcc cccgtcgagc cggttggaca ccaaaaggga ggggcaggca tggcggcata
     3361 cgcgatcatg cgatgcaaga agctggcgaa aatgggcaac gtggcggcca gtctcaagca
     3421 cgcctaccgc gagcgcgaga cgcccaacgc tgacgccagc aggacgccag agaacgagca
     3481 ctgggcggcc agcagcaccg atgaagcgat gggccgactg cgcgagttgc tgccagagaa
     3541 gcggcgcaag gacgctgtgt tggcggtcga gtacgtcatg acggccagcc cggaatggtg
     3601 gaagtcggcc agccaagaac agcaggcggc gttcttcgag aaggcgcaca agtggctggc
     3661 ggacaagtac ggggcggatc gcatcgtgac ggccagcatc caccgtgacg aaaccagccc
     3721 gcacatgacc gcgttcgtgg tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt
     3781 catcggcaac aaagcgcaga tgacccgcga ccagaccacg tttgcggccg ctgtggccga
     3841 tctagggctg caacggggca tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt
     3901 ctacgaggcc ctggagcggc caccagtggg ccacgtcacc atcagcccgc aagcggtcga
     3961 gccacgcgcc tatgcaccgc agggattggc cgaaaagctg ggaatctcaa agcgcgttga
     4021 gacgccggaa gccgtggccg accggctgac aaaagcggtt cggcaggggt atgagcctgc
     4081 cctacaggcc gccgcaggag cgcgtgagat gcgcaagaag gccgatcaag cccaagagac
     4141 ggcccgagac cttcgggagc gcctgaagcc cgttctggac gccctggggc cgttgaatcg
     4201 ggatatgcag gccaaggccg ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga
     4261 acagcgggaa gtccagcgcc agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca
     4321 tttccccgaa aagtgccacc tggcggcgtt gtgacaattt accgaacaac tccgcggccg
     4381 ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt
     4441 gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt
     4501 aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat
     4561 tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga
     4621 tatagatctc actacgcggc tgctcaaacc tgggcagaac gtaagccgcg agagcgccaa
     4681 caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc
     4741 cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac
     4801 cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc
     4861 gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa
     4921 catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     4981 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     5041 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     5101 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     5161 tgggttcgtg ccttcatccg tttccacggt gtgcgt
//
