LOCUS       sequence-232884-        4360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length CoREST with C-terminal 3xFLAG tag to be 
            expressed in a baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B,
            Golas MM
  TITLE     Stepwise assembly of functional C-terminal REST/NRSF transcriptional
            repressor complexes as a drug target.
  JOURNAL   Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 
            2017 Mar 12.
  PUBMED    28218430
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3142"; 
            journalName: "Protein Sci"; date: "2017-05"; volume: "26"; issue: 
            "5"; pages: "997-1011"
COMMENT     Sequence Label: pACEBac1-CoRESTfl
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3742..3807
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    3940..4074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3977..3996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4031..4050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4195..4360
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca tggttgaaaa gggacctgaa gtcagcggaa aacgtcgtgg aaggaacaac
     2341 gccgctgcta gtgccagcgc cgccgctgct tcagctgccg cttccgccgc ctgtgccagc
     2401 ccagccgcta ccgccgcctc tggtgctgcc gccagttcag ctagcgccgc tgctgcttcc
     2461 gctgccgctg ctccaaacaa cggtcagaat aagagtctgg ctgccgctgc cccaaacggc
     2521 aactctagct ccaactcatg ggaagaaggt agctccggta gctctagcga tgaagagcac
     2581 ggaggtggcg gtatgcgtgt gggtccccag taccaggccg tggttcctga cttcgatcct
     2641 gctaaactgg ccaggcgcag ccaggagcgc gacaatttgg gaatgctcgt ctggtcccct
     2701 aaccagaatc tgagtgaggc caagttggac gagtacatcg ctatcgccaa agagaagcat
     2761 ggctacaaca tggagcaggc cctgggtatg ctcttctggc acaagcacaa catcgagaag
     2821 tccctggccg acctgccaaa cttcacaccc ttccccgacg aatggaccgt tgaggataag
     2881 gtcctcttcg agcaagcctt ttccttccac ggcaagacat tccaccgtat ccaacaaatg
     2941 ctccctgata aatccatcgc tagcctggtc aagttctact attcttggaa aaagacacgc
     3001 acaaaaacat ctgttatgga ccgccatgct agaaaacaga agcgcgagcg cgaggagtcc
     3061 gaagatgaac tcgaagaagc taacggtaac aacccaatcg acatcgaagt cgaccagaac
     3121 aaggaatcca agaaggaagt ccctccaact gagactgtcc cacaggtgaa gaaggagaaa
     3181 cactctactc aagctaagaa ccgtgctaag cgtaagccac ccaagggaat gttcctgtct
     3241 caagaagacg tggaagccgt ttctgctaat gccaccgctg ccactaccgt tttgcgtcaa
     3301 ctggacatgg agctggtctc agtgaagcgc cagatccaga atattaagca aactaactca
     3361 gccctcaagg agaagctcga cggcggcatt gagccctaca ggctccctga ggtgatccag
     3421 aagtgcaacg ctcgttggac aaccgaagaa caactcctgg ccgtgcaagc tatcagaaag
     3481 tacggccgcg acttccaggc catctcagat gtgattggca ataagtctgt ggtgcaagtg
     3541 aagaactttt tcgttaacta caggcgtagg tttaacatcg acgaggtttt gcaagaatgg
     3601 gaggccgaac acggcaagga ggagaccaac ggaccctcaa accagaagcc cgttaagagc
     3661 cctgataact caatcaagat gcccgaagaa gaagacgagg ctcctgtgct ggacgtccgc
     3721 tacgcttcag cttcctctag agactacaaa gaccatgacg gtgattataa agatcatgac
     3781 atcgattaca aggatgacga tgacaagtaa aagcttgtcg agaagtacta gaggatcata
     3841 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3901 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3961 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     4081 ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     4141 tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag
     4201 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     4261 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     4321 ttccacccct cccagttccc aactattttg tccgcccaca
//
