LOCUS       sequence-232885-       17776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted A to G in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17776)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 17776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-05-29"; volume: "19(1):59. doi"; pages: " 
            10.1186/s13059-018-1443-z"
COMMENT     Sequence Label: pH-PABE-7-sgRNA
FEATURES             Location/Qualifiers
     source          1..17776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    647..671
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     859..881
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     873..890
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     874..890
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        957..1336
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     terminator      1444..1463
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      1687..1706
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        1719..3711
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             join(4919..4942,4943..4945,4946..9019)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8932..8940
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9032..9052
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9065..9085
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9098..9118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9777..9793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9777..9793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9790..9812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9801..9817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9825..9831,9832..9849,9850..9855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9870..9891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10082..10758
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10695..10715
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10825..11850
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    11891..12065
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12143..12167
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12592..13386
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13473..14061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13962..13981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14215..14232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14247..14387
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14313..14332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14473..14495
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14731..14925
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14991..16064)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16493..17122)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
       61 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
      121 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
      181 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
      241 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
      301 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
      361 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
      421 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
      481 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
      541 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
      601 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
      661 atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc
      721 tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      781 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      841 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctta
      901 gtaattcatc caggtcacca agttctagga ttttcagaac tgcaacttat tttatcaagg
      961 aatctttaaa catacgaaca gatcacttaa agttcttctg aagcaactta aagttatcag
     1021 gcatgcatgg atcttggagg aatcagatgt gcagtcaggg accatagcac aagacaggcg
     1081 tcttctactg gtgctaccag caaatgctgg aagccgggaa cactgggtac gttggaaacc
     1141 acgtgatgtg aagaagtaag ataaactgta ggagaaaagc atttcgtagt gggccatgaa
     1201 gcctttcagg acatgtattg cagtatgggc cggcccatta cgcaattgga cgacaacaaa
     1261 gactagtatt agtaccacct cggctatcca catagatcaa agctgattta aaagagttgt
     1321 gcagatgatc cgtggcgtga gaccctgcag ggtctctgtt taagagctat gctggaaaca
     1381 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     1441 tgcttttttt gttttttatg tctaagcttg catgcctgca gtgcagcgtg acccggtcgt
     1501 gcccctctst attagtacca cctcggctat ccacatagat caaagctgat ttaaaagagt
     1561 tgtgcagatg atccgtggcg tgagaccctg cagggtctct gtttaagagc tatgctggaa
     1621 acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1681 cggtgctttt tttgtttttt atgtctaagc ttgcatgcct gcagtgcagc gtgacccggt
     1741 cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata
     1801 ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact
     1861 ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat
     1921 ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta
     1981 cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat
     2041 ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat
     2101 agactaattt ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta
     2161 aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg
     2221 actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg
     2281 tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca
     2341 gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg
     2401 cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc
     2461 agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc
     2521 tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc
     2581 gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg
     2641 gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa
     2701 ggtacgccgc tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt
     2761 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     2821 ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     2881 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     2941 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     3001 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     3061 cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt
     3121 tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat
     3181 agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg
     3241 cgggttttac tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt
     3301 gtggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg
     3361 gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt
     3421 aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg
     3481 catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta
     3541 ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat
     3601 atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg
     3661 tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca gccctaggcc
     3721 tatgtccgag gtggagttct ctcacgagta ttggatgagg cacgctctta cacttgctaa
     3781 gagagcttgg gacgaaagag aagtgccagt tggcgccgtt cttgtgcata ataatagggt
     3841 gatcggcgag ggttggaata gaccaattgg aaggcatgat ccaacagctc acgcagagat
     3901 tatggctctc agacaaggcg gcctcgttat gcagaactac aggctcattg acgctacact
     3961 ctacgtgaca ctcgaacctt gcgttatgtg cgccggagct atgattcatt ctaggattgg
     4021 cagggtcgtg tttggagcta gggacgctaa aacaggagcc gccggatctc ttatggacgt
     4081 gttgcatcat ccaggcatga accatagggt ggagattaca gagggcattc ttgcagacga
     4141 gtgcgctgct cttctttccg atttcttcag gatgagaagg caggagatta aggcccagaa
     4201 gaaggctcag tcttctacag atagcggagg atcttccgga ggatctagcg gctccgagac
     4261 accaggaaca tccgaaagcg ctacaccaga atctagcgga ggctcttccg gaggatcttc
     4321 tgaagtggag ttctcccacg agtattggat gaggcacgct cttacacttg ctaaaagggc
     4381 tagggacgaa agggaagttc cagttggagc tgttctcgtg ctcaataaca gggtgattgg
     4441 cgagggttgg aatagagcca ttggactcca tgatccaaca gctcacgcag agattatggc
     4501 tcttagacaa ggcggcctcg ttatgcagaa ttacagactc atcgacgcca cactctacgt
     4561 taccttcgaa ccttgcgtta tgtgcgccgg agctatgatc cattctagga ttggcagggt
     4621 cgtgttcggc gttagaaacg ctaagacagg agctgcaggc tctcttatgg acgttcttca
     4681 ttacccaggc atgaatcata gagtggagat cacagaaggc attcttgcag acgagtgcgc
     4741 agctctcctt tgctatttct tcaggatgcc gaggcaagtt ttcaacgctc agaagaaggc
     4801 ccagtcttct acagattccg gcggatcttc tggaggatct agcggctccg agacaccagg
     4861 aacatccgaa tccgctacac cagagtcttc tggaggatct agcggaggat ctcttaagga
     4921 caagaagtac tcgatcggcc tcgccattgg gactaactct gttggctggg ccgtgatcac
     4981 cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg atcggcattc
     5041 catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg ctgaggctac
     5101 gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca tctgctacct
     5161 gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc acaggctgga
     5221 ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct tcggcaacat
     5281 tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc ggaagaagct
     5341 cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg cccacatgat
     5401 taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata gcgatgttga
     5461 caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga accccattaa
     5521 tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt ctcggcgcct
     5581 cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg ggaatctcat
     5641 tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg ctgaggacgc
     5701 caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc tggcccagat
     5761 cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg ccatcctcct
     5821 gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag cctccatgat
     5881 caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg tcaggcagca
     5941 gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct acgctgggta
     6001 cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga ttctggagaa
     6061 gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc tgaggaagca
     6121 gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc tgcacgctat
     6181 cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga agatcgagaa
     6241 gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca actcccgctt
     6301 cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg aggaggtggt
     6361 cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg acaagaacct
     6421 gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca cagtctacaa
     6481 cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt tcctgtctgg
     6541 ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg tcacggttaa
     6601 gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg agatctctgg
     6661 cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga agatcattaa
     6721 ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca ttgtgctgac
     6781 actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt acgcccatct
     6841 cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct gggggaggct
     6901 gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga tcctcgactt
     6961 cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg atgacagcct
     7021 cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact cgctgcacga
     7081 gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc agaccgtgaa
     7141 ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca tcgtcattga
     7201 gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg agaggatgaa
     7261 gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc acccggtgga
     7321 gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg gccgcgatat
     7381 gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg atcatatcgt
     7441 tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca ggtcggacaa
     7501 gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga tgaagaacta
     7561 ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata acctcacaaa
     7621 ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga ggcagctggt
     7681 cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga tgaacactaa
     7741 gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga agtcaaagct
     7801 cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca acaattacca
     7861 ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca agaagtaccc
     7921 aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga ggaagatgat
     7981 cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct actctaacat
     8041 tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga agcgccccct
     8101 catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg atttcgcgac
     8161 cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag aggtccagac
     8221 tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc tcatcgcccg
     8281 caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg tggcctactc
     8341 ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga gcgtgaagga
     8401 gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga tcgatttcct
     8461 ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc ccaagtactc
     8521 actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg agctgcagaa
     8581 ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg cctcccacta
     8641 cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg tcgagcagca
     8701 caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc gcgtgatcct
     8761 ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg acaagccaat
     8821 cagggagcag gctgagaata tcattcatct cttcaccctg acgaacctcg gcgcccctgc
     8881 tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca ctaaggaggt
     8941 cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc gcatcgacct
     9001 gtctcagctc gggggcgact ccggcggcag cccaaagaag aagcggaagg tgtctggagg
     9061 ttctcctaag aaaaagagaa aagtgtccgg cggctccccg aagaagaagc gcaaggtgta
     9121 ggagctcaga gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat
     9181 cagtttcatt gcgcacacac cagaatccta ctgagtttga gtattatggc attgggaaaa
     9241 ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg
     9301 ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg
     9361 ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa
     9421 ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa
     9481 tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc ttattcacta
     9541 ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata caagtatgtc
     9601 ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa tccttgtcag
     9661 attctaatca ttgctttata attatagtta tactcatgga tttgtagttg agtatgaaaa
     9721 tattttttaa tgcattttat gacttgccaa ttgattgaca acgaattcgt aatcatgtca
     9781 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9841 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9901 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9961 caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca gcttgccaac atggtggagc
    10021 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
    10081 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
    10141 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
    10201 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
    10261 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
    10321 tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat
    10381 acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac
    10441 ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa
    10501 ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct
    10561 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
    10621 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
    10681 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
    10741 ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt
    10801 cgcagatccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
    10861 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
    10921 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
    10981 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
    11041 ccgattccgg aagtgcttga cattggggag tttagcgaga gcctgaccta ttgcatctcc
    11101 cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttcta
    11161 caaccggtcg cggaggctat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
    11221 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
    11281 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
    11341 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
    11401 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
    11461 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
    11521 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
    11581 catccggagc ttgcaggatc gccacgactc cgggcgtata tgctccgcat tggtcttgac
    11641 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
    11701 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
    11761 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
    11821 cccagcactc gtccgagggc aaagaaatag agtagatgcc gaccgggatc tgtcgatcga
    11881 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
    11941 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
    12001 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
    12061 agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
    12121 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
    12181 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
    12241 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
    12301 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
    12361 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat
    12421 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
    12481 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
    12541 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
    12601 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
    12661 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
    12721 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
    12781 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
    12841 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
    12901 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
    12961 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
    13021 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
    13081 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
    13141 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
    13201 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
    13261 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
    13321 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
    13381 ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    13441 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    13501 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    13561 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    13621 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    13681 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    13741 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    13801 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    13861 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    13921 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    13981 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    14041 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    14101 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    14161 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    14221 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
    14281 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    14341 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
    14401 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    14461 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    14521 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
    14581 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
    14641 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
    14701 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
    14761 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
    14821 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
    14881 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa cagacctttt
    14941 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
    15001 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
    15061 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
    15121 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
    15181 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
    15241 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
    15301 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
    15361 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
    15421 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
    15481 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
    15541 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
    15601 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
    15661 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
    15721 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
    15781 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
    15841 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
    15901 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
    15961 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
    16021 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    16081 tgggggttcc agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac
    16141 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    16201 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    16261 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    16321 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    16381 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    16441 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    16501 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    16561 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    16621 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    16681 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    16741 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    16801 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    16861 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    16921 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    16981 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    17041 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    17101 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    17161 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    17221 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    17281 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    17341 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    17401 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    17461 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    17521 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    17581 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    17641 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    17701 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    17761 aacacggtca acacca
//
