LOCUS       sequence-232899-       11210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of TP901-1 attP-flanked platforms for 
            cassette exchange using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Voutev R, Mann RS
  TITLE     TP901-1 Phage Recombinase Facilitates Genome Engineering in 
            Drosophila melanogaster.
  JOURNAL   G3 (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 
            10.1534/g3.119.0002.
  PUBMED    30696702
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 
            10.1534/g3.119.0002."
COMMENT     Sequence Label: pRVV688
FEATURES             Location/Qualifiers
     source          1..11210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6722..7174
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7121..7142
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             7213..8007
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7267..7286)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7877..7896
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8375..8440
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8570..8590
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8862..8996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8899..8918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8953..8972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(9022..9040)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9061..9077)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9061..9077)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9074..9096)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9085..9101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9109..9115,9116..9133,9134..9139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9154..9175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9292..9309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9463..10051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9543..9562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10222..11013,11014..11082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10845..10864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11083..11187)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag atttaaatgc
     4801 atgccctgca ggaagcttcc taggaggcct agatctgcgg ccgctccaac tcgcttaatt
     4861 gcgagttttt atttcgttta tttcaattaa ggtaactaaa aaactccttt taattaaccg
     4921 cgagctcgcc cggggatcta attcaattag agactaattc aattagagct aattcaatta
     4981 ggatccaagc ttatcgattt cgaaccctcg accgccggag tataaataga ggcgcttcgt
     5041 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     5101 gcaaataaac aagcgcagct gaacaagcta aacaatcggg cggccgcact agagccggtc
     5161 gccaccatga ggtcttccaa gaatgttatc aaggagttca tgaggtttaa ggttcgcatg
     5221 gaaggaacgg tcaatgggca cgagtttgaa atagaaggcg aaggagaggg gaggccatac
     5281 gaaggccaca ataccgtaaa gcttaaggta accaaggggg gacctttgcc atttgcttgg
     5341 gatattttgt caccacaatt tcagtatgga agcaaggtat atgtcaagca ccctgccgac
     5401 ataccagact ataaaaagct gtcatttcct gaaggattta aatgggaaag ggtcatgaac
     5461 tttgaagacg gtggcgtcgt tactgtaacc caggattcca gtttgcagga tggctgtttc
     5521 atctacaagg tcaagttcat tggcgtgaac tttccttccg atggacctgt tatgcaaaag
     5581 aagacaatgg gctgggaagc cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa
     5641 ggagagattc ataaggctct gaagctgaaa gacggtggtc attacctagt tgaattcaaa
     5701 agtatttaca tggcaaagaa gcctgtgcag ctaccagggt actactatgt tgactccaaa
     5761 ctggatataa caagccacaa cgaagactat acaatcgttg agcagtatga aagaaccgag
     5821 ggacgccacc atctgttcct ttagcggcca tcgaattcga gctcgcccac taagcgtcgc
     5881 gccacttcaa cgctcgatgg gagcgtcatt ggtgggcggg gtaaccgtcg aaatcagtgt
     5941 ttacgcttcc aatcgcaaca aaaaattcac tgcaacactg aaaagcatac gaaaacgatg
     6001 aagattgtac gagaaaccat aaagtatttt atccacaaag acacgtatag cagaaaagcc
     6061 aagttaactc ggcgataagt tgtgtacaca agaataaaat cggccagatt cagtgttgtc
     6121 agaaataaga aaaccccact atgtttttct ttgccttttc tttctcccag cgatcattca
     6181 tttcgtggtg aaagaacggg gtcattgcac ggagtttcga ctgcgggaaa gcagagctgc
     6241 cgttcacttc gtctataatt agcgctttct attttccccg attcgggccg ctgctgcgct
     6301 tttccgcctg ctgtttgtgg caagtgtagc agcaggctgt gcacgcagtg tggcatgcac
     6361 ttggctttcc accgttggta tcgattctct gggacgatga gtcattcctt tcggggccac
     6421 agcataatcg ttgccagctc accgaaatgg tgacttcatt tcttaactgc cgtcaagcat
     6481 gcgattgtac atacatacat atttatatat gtacatattt atgtgactat ggtaggtcga
     6541 tataatagca atcaacgcaa gcaaatgtgt cagtcctgct tacaggaacg attctattta
     6601 gtaattttcg ttgtataaag taattatgta tgtatgtaag ccccataaat ctgaaacaat
     6661 taggcaaaac catgcgaagc tcaattcgaa gttcctattc ttcaaaaggt ataggaactt
     6721 cctagaatcc caaaacaaac tggttattgt ggtaggtcat ttgtttggca gaaagaaaac
     6781 tcgagaaatt tctctggccg ttattcgtta ttctctcttt tctttttggg tctctccctc
     6841 tctgcactaa tgctctctca ctctgtcaca cagtaaacgg catactgctc tcgttggttc
     6901 gagagagcgc gcctcgaatg ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg
     6961 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
     7021 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag
     7081 tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca
     7141 agaagtaatt attgaataca agaagagaac tctgggggat ctgatcaaga gacaggatga
     7201 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7261 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7321 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7381 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7441 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7501 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7561 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7621 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7681 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7741 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7801 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7861 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7921 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7981 tatcgccttc ttgacgagtt cttctgagaa gttcctattc ttcaaaaggt ataggaactt
     8041 caccggtaag gagtttttta gttaccttaa ttgaaataaa cgaaataaaa actcgcaatt
     8101 aagcgagttg gaggcgcgcc gctagccata tggggattgg tggcgacgac tcctggagcc
     8161 cgtcagtatc ggcggaatta cagctgagcg ccggtcgcta ccattaccag ttggtctggt
     8221 gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatccc
     8281 tcgagggtac ctctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8341 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8401 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8461 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8521 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8581 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     8641 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8701 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
     8761 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     8821 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     8881 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8941 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccact
     9001 agttctagcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
     9061 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
