LOCUS       sequence-232904-       10238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of TP901-1 attP-flanked platforms for 
            cassette exchange using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Voutev R, Mann RS
  TITLE     TP901-1 Phage Recombinase Facilitates Genome Engineering in 
            Drosophila melanogaster.
  JOURNAL   G3 (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 
            10.1534/g3.119.0002.
  PUBMED    30696702
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 
            10.1534/g3.119.0002."
COMMENT     Sequence Label: pRVV700
FEATURES             Location/Qualifiers
     source          1..10238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     CDS             join(6813..6815,6816..6818,6819..7532)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6858..6879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7119..7138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7466..7487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7554..7675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7565..7584)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7619..7638
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8050..8068)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8089..8105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8089..8105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8102..8124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8113..8129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8137..8143,8144..8161,8162..8167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8182..8203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8320..8337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8491..9079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8571..8590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9250..10041,10042..10110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9873..9892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10111..10215)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag atttaaatgc
     4801 atgccctgca ggaagcttcc taggaggcct agatctgcgg ccgctccaac tcgcttaatt
     4861 gcgagttttt atttcgttta tttcaattaa ggtaactaaa aaactccttt taattaagaa
     4921 gttcctattc ttcaaaaggt ataggaactt ctctagcccg gcttgctgtt cttcgcgttc
     4981 aaaatctcca gctccatttt gctttcggtg cgcttgcaat cagtactgtc caaaatcgaa
     5041 aatcgccgaa ccgtagtgtg accgtgcggg gctctgcgaa aataaacttt tttaggtata
     5101 tggccacaca cggggaaagc acagtggatt atatgtttta atattataat atgcaggttt
     5161 tcattactta tccagatgta agcccactta aagcgattta acaattattt gccgaaagag
     5221 tataaacaaa tttcacttaa aaatggatta agaaaagctt gtgtaagatt atgcgcagcg
     5281 ttgccagata gctccattta aaacacttca aaaacaataa gttttgaaaa tatatacata
     5341 aatagcagtc gttgccgcaa cgctcaacac atcacacttt taaaacaccc tttacctaca
     5401 cagaattact ttttaaattt ccagtcaagc tgcgagtttc aaaattatag ccggtagaga
     5461 agacagtgct atttcaaaag caaactaaca agggtcttaa attccaaaac accaatccta
     5521 acaagccttg gacttttgta agtttagatc aaaggtggca ttgcattcaa tgtcatggta
     5581 agaagtaggt cgtctaggta gaaatcctca ttcagccggt caagtcagta cgagaaaggt
     5641 ctcaatttga aattgtctta aaaatatttt attgttttgt actgtggtga gtttaaacga
     5701 aaaacacaaa aaaaaagtga tacacagaaa tcataaaaaa ttttaataca aggtattcgt
     5761 acgtatcaaa aacatttcgg cacaattttt tttctctgta ctaaagtgtt acgaacacta
     5821 cggtattttt tagtgatttt caacggacac cgaaggtata taaacagcgt tcgcgaacgg
     5881 tcgccttcaa aaccaattga catttgcagc agcaagtaca agtagaaagt aaagcgcaat
     5941 cagcgaaaaa tttatactta attgttggtg attaaagtac aattaaaaga acattctcga
     6001 aagtcacaag aaacgtaagt ttttaactcg ctgttaccaa ttagtaataa gagcaacaag
     6061 acgttgagta atttcaagaa aaactgcatt tcaaggtctt tgttcggcca ttttttttta
     6121 ttcaacgctc tacgtaatta caaaataaga aattggcagc cacgcatctt gttttcccaa
     6181 tgaattggca tcaaaacgca aacaaatcta taaataaaac ttgcgtgttg attttcgcca
     6241 agatttattg gcaaattgtg aaattcgcag tgacgcattt gaaaattcga gaaatcacga
     6301 acgcactcga gcatttgtgt gcatgttatt agttagttgt ttagttaatt gaagtatttt
     6361 accaacgaaa tccacttatt tttagctgaa atagagtagg ttgcttaaac aaagccacgt
     6421 ctgaaaattt cttattgctt gtagttgtga cgtcaccata tacacacaaa ataatgtgta
     6481 tgcatgcatt tcagctgtgt atatatacat gcacacactc gcaacacgaa aacgatgacg
     6541 aagcaacgga acaaaggttt ctcaactacc ctttgttccc tgtttcttcg ctttcctttg
     6601 ttccaatatt cgtagagggt taataggggt ttctcaacaa agttggcgtc gataaataag
     6661 tttcccattt ttattcccca gccaggaagt tagtttcaat agttttgtaa tttcaacgaa
     6721 actcatttga tttcgtacta attttccaca tctctatttt ctgcccgcag aataatccaa
     6781 gggcggatcc cccgggctgc aggaattcaa aaatggtgag caagggcgag gagctgttca
     6841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     6901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     6961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     7021 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     7081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     7141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     7201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     7261 acgtctatat catggccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     7321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     7381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     7441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     7501 tcactctcgg catggacgag ctgtacaagt agggtacctc tagacagaca tgataagata
     7561 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7621 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7681 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7741 cgaagttcct attcttcaaa aggtatagga acttcaccgg taaggagttt tttagttacc
     7801 ttaattgaaa taaacgaaat aaaaactcgc aattaagcga gttggaggcg cgccgctagc
     7861 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attacagctg
     7921 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     7981 ccatgtctgc ccgtatttcg cgtaaggaaa tccctcgagg gtacctctag ttctagccag
     8041 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     8101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10201 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
