LOCUS       sequence-232931-        3603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PYL1-SfGFP-Coilin, lentivirus vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-PGK-PYL1-sfGFP-Coilin
FEATURES             Location/Qualifiers
     source          1..3603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..504
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(40..63)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     437..456
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1138..1848
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1138..1848
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1165..1193)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1788..1808
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
       61 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      241 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
      361 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      421 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      481 tcttcctcat ctccgggcct ttcgacctgc agcccaagct tacgcgtctg caggatatca
      541 agcttgcggt acgactgagt catgggtggg ggcgcgccaa ctcaagacga attcacccaa
      601 ctctcccaat caatcgccga gttccacacg taccaactcg gtaacggccg ttgctcatct
      661 ctcctagctc agcgaatcca cgcgccgccg gaaacagtat ggtccgtggt gagacgtttc
      721 gataggccac agatttacaa acacttcatc aaaagctgta acgtgagtga agatttcgag
      781 atgcgagtgg gatgcacgcg cgacgtgaac gtgataagtg gattaccggc gaatacgtct
      841 cgagagagat tagatctgtt ggacgatgat cggagagtga ctgggtttag tataaccggt
      901 ggtgaacata ggctgaggaa ttataaatcg gttacgacgg ttcatagatt tgagaaagaa
      961 gaagaagaag aaaggatctg gaccgttgtt ttggaatctt atgttgttga tgtaccggaa
     1021 ggtaattcgg aggaagatac gagattgttt gctgatacgg ttattagatt gaatcttcag
     1081 aaacttgctt cgatcactga agctatgaac gtggatccag gtggaggtgg aagcggtagc
     1141 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1201 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     1261 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     1321 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     1381 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     1441 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     1501 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     1561 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     1621 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     1681 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     1741 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     1801 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg tggaggtcgg
     1861 accgcaagca agatggcagc ttccgagacg gttaggctac ggcttcaatt tgattacccg
     1921 ccgccagcta ccccgcactg tacggccttc tggcttctgg tcgacttgaa cagatgccga
     1981 gtcgtcacag atctcattag tctcatccgc cagcgcttcg gcttcagttc tggggccttc
     2041 ctaggcctct acctggaggg ggggctcttg ccccccgccg agagcgcgcg ccttgtgaga
     2101 gacaacgact gcctcagagt taaattagaa gagagaggag ttgctgagaa ttctgtagtc
     2161 atcagtaatg gtgacattaa tttatctctt agaaaagcaa agaagcgggc atttcagtta
     2221 gaggagggtg aagaaactga accagattgc aaatattcaa agaagcattg gaagagtcga
     2281 gagaacaata acaataatga gaaggtcttg gatctggaac caaaagctgt cacagatcag
     2341 actgtcagca aaaaaaacaa gagaaaaaat aaagcaacct gtggcacagt gggtgatgat
     2401 aacgaagagg ccaaaagaaa atcaccaaag aaaaaggaga aatgtgaata taaaaaaaag
     2461 gctaagaatc ccaagtctcc gaaagtacag gcagtgaaag actgggccaa tcagagatgt
     2521 agttctccaa aaggttctgc tagaaacagc cttgttaaag ccaaaaggaa aggtagtgta
     2581 agcgtttgct caaaagagag tcccagttcc tcctcggagt ctgagtcttg tgatgaatct
     2641 atcagtgatg gtcccagcaa agtcactttg gaggccagaa attcctcaga gaaattacca
     2701 actgagttat caaaggaaga accctctacc aaaaatacaa ctgcagacaa actggctata
     2761 aaacttggct ttagccttac ccccagcaag ggcaagacct ctggaacaac atcttccagt
     2821 tcagactcta gtgcagagtc agacgaccaa tgcttgatgt catcgagcac cccggagtgt
     2881 gctgcgggtt tcttaaagac agtaggcctt tttgcaggaa gaggtcgtcc aggcccaggg
     2941 ctgtcatcac agactgcagg tgctgctgga tggaggcgtt ctggctcaaa tggtggtgga
     3001 caggctcctg gtgcttctcc cagtgtgtct ctccctgcta gtttaggaag aggatggggt
     3061 agagaagaga accttttttc ttggaaggga gctaagggac ggggcatgcg ggggagaggt
     3121 cgaggacgag ggcatcctgt ttcctgtgtt gtaaatagaa gcactgacaa ccagaggcaa
     3181 cagcaattaa atgacgtggt aaaaaattca tctactatta tccagaatcc agtagagaca
     3241 cccaagaagg actatagtct gttaccactg ttagcagctg cccctcaagt tggagaaaag
     3301 attgcattta agcttttgga gctaacatcc agttactctc ctgatgtctc tgactacgag
     3361 gaaggaagaa tattaagcca caatccagag acccagcaag tagatataga aattctttca
     3421 tccttacctg ccttgagaga acctgggaaa tttgatttag tttatcacaa tgaaaatgga
     3481 gctgaggtag tggagtacgc tgtgacacag gagagcaaga tcactgtatt ttggaaagag
     3541 ttgattgacc caagactgat tattgaatct ccaagtaaca catcaagtac agaacctgcc
     3601 tga
//
