LOCUS       sequence-232932-       12550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human BRCA1 and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 12550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-BRCA1
FEATURES             Location/Qualifiers
     source          1..12550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1689..1708
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1689..1707
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1712..1721
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7327..7343)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7363..7587
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          7808..8746
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8703..8723
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8781..8790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8787..8789,8790..8792,8793..9506)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(8832..8853)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    9537..9648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9766..9783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9766..9782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9775..9797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9923..10378)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10060..10081)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10272..10291
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10405..10509
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10510..10578,10579..11370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10728..10747)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11541..12129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12030..12049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12283..12300
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12417..12438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12453..12458,12459..12476,12477..12483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12491..12507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12496..12518
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12515..12531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12515..12531
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggsggggs grggggsggg
      541 gsggggcgrg gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggrg ggctcggggg rggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagatcc
     1681 ttaattaata atacgactca ctataggggc cgccaccatg gatttatctg ctcttcgcgt
     1741 tgaagaagta caaaatgtca ttaatgctat gcagaaaatc ttagagtgtc ccatctgtct
     1801 ggagttgatc aaggaacctg tctccacaaa gtgtgaccac atattttgca aattttgcat
     1861 gctgaaactt ctcaaccaga agaaagggcc ttcacagtgt cctttatgta agaatgatat
     1921 aaccaaaagg agcctacaag aaagtacgag atttagtcaa cttgttgaag agctattgaa
     1981 aatcatttgt gcttttcagc ttgacacagg tttggagtat gcaaacagct ataattttgc
     2041 aaaaaaggaa aataactctc ctgaacatct aaaagatgaa gtttctatca tccaaagtat
     2101 gggctacaga aaccgtgcca aaagacttct acagagtgaa cccgaaaatc cttccttgca
     2161 ggaaaccagt ctcagtgtcc aactctctaa ccttggaact gtgagaactc tgaggacaaa
     2221 gcagcggata caacctcaaa agacgtctgt ctacattgaa ttgggatctg attcttctga
     2281 agataccgtt aataaggcaa cttattgcag tgtgggagat caagaattgt tacaaatcac
     2341 ccctcaagga accagggatg aaatcagttt ggattctgca aaaaaggctg cttgtgaatt
     2401 ttctgagacg gatgtaacaa atactgaaca tcatcaaccc agtaataatg atttgaacac
     2461 cactgagaag cgtgcagctg agaggcatcc agaaaagtat cagggtagtt ctgtttcaaa
     2521 cttgcatgtg gagccatgtg gcacaaatac tcatgccagc tcattacagc atgagaacag
     2581 cagtttatta ctcactaaag acagaatgaa tgtagaaaag gctgaattct gtaataaaag
     2641 caaacagcct ggcttagcaa ggagccaaca taacagatgg gctggaagta aggaaacatg
     2701 taatgatagg cggactccca gcacagaaaa aaaggtagat ctgaatgctg atcccctgtg
     2761 tgagagaaaa gaatggaata agcagaaact gccatgctca gagaatccta gagatactga
     2821 agatgttcct tggataacac taaatagcag cattcagaaa gttaatgagt ggttttccag
     2881 aagtgatgaa ctgttaggtt ctgatgactc acatgatggg gagtctgaat caaatgccaa
     2941 agtagctgat gtattggacg ttctaaatga ggtagatgaa tattctggtt cttcagagaa
     3001 aatagactta ctggccagtg atcctcatga ggctttaata tgtaaaagtg aaagagttca
     3061 ctccaaatca gtagagagta atattgaaga caaaatattt gggaaaacct atcggaagaa
     3121 ggcaagcctc cccaacttaa gccatgtaac tgaaaatcta attataggag catttgttac
     3181 tgagccacag ataatacaag agcgtcccct cacaaataaa ttaaagcgta aaaggagacc
     3241 tacatcaggc cttcatcctg aggattttat caagaaagca gatttggcag ttcaaaagac
     3301 tcctgaaatg ataaatcagg gaactaacca aacggagcag aatggtcaag tgatgaatat
     3361 tactaatagt ggtcatgaga ataaaacaaa aggtgattct attcagaatg agaaaaatcc
     3421 taacccaata gaatcactcg aaaaagaatc tgctttcaaa acgaaagctg aacctataag
     3481 cagcagtata agcaatatgg aactcgaatt aaatatccac aattcaaaag cacctaaaaa
     3541 gaataggctg aggaggaagt cttctaccag gcatattcat gcgcttgaac tagtagtcag
     3601 tagaaatcta agcccaccta attgtactga attgcaaatt gatagttgtt ctagcagtga
     3661 agagataaag aaaaaaaagt acaaccaaat gccagtcagg cacagcagaa acctacaact
     3721 catggaaggt aaagaacctg caactggagc caagaagagt aacaagccaa atgaacagac
     3781 aagtaaaaga catgacagtg atactttccc agagctgaag ttaacaaatg cacctggttc
     3841 ttttactaag tgttcaaata ccagtgaact taaagaattt gtcaatccta gccttccaag
     3901 agaagaaaaa gaagagaaac tagaaacagt taaagtgtct aataatgctg aagaccccaa
     3961 agatctcatg ttaagtggag aaagggtttt gcaaactgaa agatctgtag agagtagcag
     4021 tatttcactg gtacctggta ctgattatgg cactcaggaa agtatctcgt tactggaagt
     4081 tagcactcta gggaaggcaa aaacagaacc aaataaatgt gtgagtcagt gtgcagcatt
     4141 tgaaaacccc aagggactaa ttcatggttg ttccaaagat aatagaaatg acacagaagg
     4201 ctttaagtat ccattgggac atgaagttaa ccacagtcgg gaaacaagca tagaaatgga
     4261 agaaagtgaa cttgatgctc agtatttgca gaatacattc aaggtttcaa agcgccagtc
     4321 atttgctctg ttttcaaatc caggaaatgc agaagaggaa tgtgcaacat tctctgccca
     4381 ctctgggtcc ttaaagaaac aaagtccaaa agtcactttt gaatgtgaac aaaaggaaga
     4441 aaatcaagga aagaatgagt ctaatatcaa gcctgtacag acagttaata tcactgcagg
     4501 ctttcctgtg gttggtcaga aagataagcc agttgataat gccaaatgta gtatcaaagg
     4561 aggctctagg ttttgtctat catctcagtt cagaggcaac gaaactggac tcattactcc
     4621 aaataaacat ggacttttac aaaacccata tcgtatacca ccactttttc ccatcaagtc
     4681 atttgttaaa actaaatgta agaaaaatct gctagaggaa aactttgagg aacattcaat
     4741 gtcacctgaa agagaaatgg gaaatgagaa cattccaagt acagtgagca caattagccg
     4801 taataacatt agagaaaatg tttttaaagg agccagctca agcaatatta atgaagtagg
     4861 ttccagtact aatgaagtgg gctccagtat taatgaaata ggttccagtg atgaaaacat
     4921 tcaagcagaa ctaggtagaa acagagggcc aaaattgaat gctatgctta gattaggggt
     4981 tttgcaacct gaggtctata aacaaagtct tcctggaagt aattgtaagc atcctgaaat
     5041 aaaaaagcaa gaatatgaag aagtagttca gactgttaat acagatttct ctccatatct
     5101 gatttcagat aacttagaac agcctatggg aagtagtcat acatctcagg tttgttctga
     5161 gacacctgat gacctgttag atgatggtga aataaaggaa gatactagtt ttgctgaaaa
     5221 tgacattaag gaaagttctg ctgtttttag caaaagcgtc cagagaggag agcttagcag
     5281 gagtcctagc cctttcaccc atacacattt ggctcagggt taccgaagag gggccaagaa
     5341 attagagtcc tcagaagaga acttatctag tgaggatgaa gagcttccct gcttccaaca
     5401 cttgttattt ggtaaagtaa acaatatacc ttctcagtct actaggcata gcaccgttgc
     5461 taccgagtgt ctgtctaaga acacagagga gaatttatta tcattgaaga atagcttaaa
     5521 tgactgcagt aaccaggtaa tattggcaaa ggcatctcag gaacatcacc ttagtgagga
     5581 aacaaaatgt tctgctagct tgttttcttc acagtgcagt gaattggaag acttgactgc
     5641 aaatacaaac acccaggatc ctttcttgat tggttcttcc aaacaaatga ggcatcagtc
     5701 tgaaagccag ggagttggtc tgagtgacaa ggaattggtt tcagatgatg aagaaagagg
     5761 aacgggcttg gaagaaaata atcaagaaga gcaaagcatg gattcaaact taggtgaagc
     5821 agcatctggg tgtgagagtg aaacaagcgt ctctgaagac tgctcagggc tatcctctca
     5881 gagtgacatt ttaaccactc agcagaggga taccatgcaa cataacctga taaagctcca
     5941 gcaggaaatg gctgaactag aagctgtgtt agaacagcat gggagccagc cttctaacag
     6001 ctaccctagt ataatcagtg actcttctgc ccttgaggac ctgcgaaatc cagaacaaag
     6061 cacatcagaa aaagcagtat taacttcaca gaaaagtagt gaatacccta taagccagaa
     6121 tccagaaggc ctttctgctg acaagtttga ggtgtctgca gatagttcta ccagtaaaaa
     6181 taaagaacca ggagtggaaa ggtcatcccc ttctaaatgc ccatcattag atgataggtg
     6241 gtacatgcac agttgctctg ggagtcttca gaatagaaac tacccatctc aagaggagct
     6301 cattaaggtt gttgatgtgg aggagcaaca gctggaagag tctgggccac acgatttgac
     6361 ggaaacatct tacttgccaa ggcaagatct agagggaacc ccttacctgg aatctggaat
     6421 cagcctcttc tctgatgacc ctgaatctga tccttctgaa gacagagccc cagagtcagc
     6481 tcgtgttggc aacataccat cttcaacctc tgcattgaaa gttccccaat tgaaagttgc
     6541 agaatctgcc cagggtccag ctgctgctca tactactgat actgctgggt ataatgcaat
     6601 ggaagaaagt gtgagcaggg agaagccaga attgacagct tcaacagaaa gggtcaacaa
     6661 aagaatgtcc atggtggtga gtggcctgac cccagaagaa tttatgctcg tgtacaagtt
     6721 tgccagaaaa caccacatca ctttaactaa tctaattact gaagagacta ctcatgttgt
     6781 tatgaaaaca gatgctgagt ttgtgtgtga acggacactg aaatattttc taggaattgc
     6841 gggaggaaaa tgggtagtta gctatttctg ggtgacccag tctattaaag aaagaaaaat
     6901 gctgaatgag catgattttg aagtcagagg agatgtggtc aatggaagaa accaccaagg
     6961 tccaaagcga gcaagagaat cccaggacag aaagatcttc agggggctag aaatctgttg
     7021 ctatgggccc ttcaccaaca tgcccacaga tcaactggaa tggatggtac agctgtgtgg
     7081 tgcttctgtg gtgaaggagc tttcatcatt cacccttggc acaggtgtcc acccaattgt
     7141 ggttgtgcag ccagatgcct ggacagagga caatggcttc catgcaattg ggcagatgtg
     7201 tgaggcacct gtggtgaccc gagagtgggt gttggacagt gtagcactct accagtgcca
     7261 ggagctggac acctacctga taccccagat cccccacagc cactactgaa cgcgtaaatg
     7321 attgcagatc cactagttct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     7381 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7441 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7501 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7561 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     7621 gatccactag ttctagcctc gaggctagag cggccccggt gcctagagaa ggtggcgcgg
     7681 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     7741 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     7801 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     7861 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     7921 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     7981 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     8041 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     8101 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     8161 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     8221 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     8281 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     8341 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     8401 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     8461 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     8521 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     8581 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     8641 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     8701 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgagcgat
     8761 cgcatcgaca gtgtttaaac gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     8821 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgaggg
     8881 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg
     8941 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagccacggc gtgcagtgct
     9001 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     9061 gctacgtcca ggagcgcacc atcttcttca aggacgacgg cacctacaag acccgcgccg
     9121 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc gtcgacttca
     9181 aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacatct
     9241 atatcatggc cgtcaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaacg
     9301 tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     9361 gccccgtgct gctgcccgac agccactacc tgagcaccca gtccgtgctg agcaaagacc
     9421 ccaacgagaa gcgcgatcac atggtcctgc tggagttccg caccgccgcc gggatcactc
     9481 tcggcatgga cgagctgtac aagtgagaat tcctagagct cgctgatcag cctcgactgt
     9541 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     9601 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     9661 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     9721 agagaatagc aggcatgcgt ttaaacatta gtcgacaggg ccgccactgg ccgtcgtttt
     9781 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     9841 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     9901 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     9961 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    10021 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    10081 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    10141 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    10201 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    10261 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    10321 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
    10381 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10441 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10501 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    10561 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    10621 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    10681 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    10741 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    10801 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    10861 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    10921 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    10981 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    11041 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    11101 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    11161 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    11221 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    11281 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    11341 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    11401 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    11461 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    11521 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    11581 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    11641 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    11701 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    11761 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    11821 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    11881 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    11941 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    12001 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    12061 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    12121 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    12181 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    12241 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    12301 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    12361 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    12421 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    12481 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgaggcg
    12541 cgccggattc
//
