LOCUS       sequence-232935-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase 
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.23 EC10-AP-His
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..26
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18..35
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19..35
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        65..448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        449..652
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     603..623
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     649..673
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          788..1613
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6185..6202
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6228..6349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6265..6284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6319..6338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6387..6404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6558..7146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6638..6657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7317..8108,8109..8177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7940..7959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8178..8282)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8350..8368
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8406..8428)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8430..8450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8435..8764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8615..8750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8677..8696
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8901..8920
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8987..9442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9124..9145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9336..9355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtcgaggtct cgacggtatc
       61 gattgacatt gattattgac tagctagtta ttaatagtaa tcaattacgg ggtcattagt
      121 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      301 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
      361 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
      421 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
      481 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      541 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
      601 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      661 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      721 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      781 gagtgacgta agtaccgcct atagactcta taggcacacc cctttggctc ttatgcatgc
      841 tatactgttt ttggcttggg gcctatacac ccccgcttcc atatgctata ggtgatggta
      901 tagcttagcc tataggtgtg ggttattgac cattattgac cactccccta ttggtgacga
      961 tactttccat tactaatcca taacatggct ctttgccaca actatctcta ttggctatat
     1021 gccaatactc tgtccttcag agactgacac ggactctgta tttttacagg atggggtccc
     1081 atttattatt tacaaattca catatacaac aacgccgtcc cccgtgcccg cagtttttat
     1141 taaacatagc gtgggatctc cacgcgaatc tcgggtacgt gttccggaca tgggctcttc
     1201 tccggtagcg gcggagcttc cacatccgag ccctggtccc atgcctccag cggctcatgg
     1261 tcgctcggca gctccttgct cctaacagtg gaggccagac ttaggcacag cacaatgccc
     1321 accaccacca gtgtgccgca caaggccgtg gcggtagggt atgtgtctga aaatgagctc
     1381 ggagattggg ctcgcaccgc tgacgcagat ggaagactta aggcagcggc agaagaagat
     1441 gcaggcagct gagttgttgt attctgataa gagtcagagg taactcccgt tgcggtgctg
     1501 ttaacggtgg agggcagtgt agtctgagca gtactcgttg ctgccgcgcg cgccaccaga
     1561 cataatagct gacagactaa cagactgttc ctttccatgg gtcttttctg cagaagcttg
     1621 aattctcgag gatccgcggc cgctactgcc aacttcacac aacccgctca gcggaaacac
     1681 ggcatacgaa atgaatatgc ccaacgaacg cctcaaatgg ctaatgctgt tcgcagctgt
     1741 tgccctcatt gcttgtggta gtcagaccct agctgccaat cccccagatg ccgaccaaaa
     1801 aggacccgtc ttcctcaagg aacccaccaa ccgcattgac ttctccaact ccacgggcgc
     1861 agagatcgag tgcaaggcca gcggcaatcc catgcccgag attatttgga tcaggagcga
     1921 cggtaccgcc gtgggtgatg tgcccggatt gcgtcagatc tcatccgacg gcaagctggt
     1981 cttccctccc ttccgcgccg aggactaccg ccaggaggtc catgcccagg tgtacgcctg
     2041 cctggcccgc aaccagttcg gatccattat ctcccgggac gtccatgtgc gagccgttgt
     2101 caatcagttc tatgaggcgg agattatgac cgagtatgtt atcaggggta atgctgccgt
     2161 tctgaagtgt tccatcccgt cgttcgtggc tgattttgtt cgagttgagt cctggatcga
     2221 tgatgagggc aatgtcttgt ccttttcgga caattacgat ggaaagtatc tggtattgcc
     2281 ctctggagag ctgcacatcc gtgaggttgg acccgaggac ggatacaaga gctaccagtg
     2341 ccgaaccaaa catcgtctga ccggagaaac ccgattaagt gccacaaaag gacgattggt
     2401 catcacagaa cctgtgggca gtgttgcgcc taagatcagc gtagaaaatc gtctgaaaaa
     2461 tgcagaggct cgaattggtt ccaccttcac cctcttttgc cctggtcaag cctatccagt
     2521 acccttcttt agatggtaca agttcatcga aggaacaacc cgtaaacaag ctgtggtgct
     2581 aaatgaccgt gtgaagcagg tctctggtac tctcattatt aaggacgcag tagtcgagga
     2641 ctccggaaaa tacctgtgcg tggtgaacaa ttccgtggga ggcgaaagcg ttgagactgt
     2701 gcttaccgtc actgcccccc tatccgctaa gatcgatcct cccacacaga cagttgactt
     2761 cggacgcccc gccgtattca cttgccagta cactggaaat cccatcaaga ccgttagctg
     2821 gatgaaggac ggcaaggcta tcggacacag cgaacccgtg ctgcgcatcg aatccgtgaa
     2881 gaaggaggac aagggcatgt accagtgctt cgtaagaaac gaccaggaat cggcggaggc
     2941 gagtgctgag ctgaagctcg gaggccgttt cgatcccccc gtcatccgac aggccttcca
     3001 ggaagagacc atggaacccg gaccaagtgt attcctcaag tgcgtcgccg gcggaaaccc
     3061 cacgcccgaa atttcctggg aactcgacgg caagaaaatc gccaacaacg atcgctatca
     3121 ggtcggacag tatgtgacgg tcaacggaga tgtggtttcc tatctgaaca taacctcggt
     3181 ccacgccaac gatggcggtc tctacaagtg catagccaag agcaaagtgg gcgtggccga
     3241 acactcggcc aagttgaatg tatatggact gccctacatc cggcaaatgg agaagaaggc
     3301 aattgttgct ggagaaaccc tgattgtcac atgtcctgta gctggatacc ccatcgattc
     3361 gattgtctgg gagcgagata accgtgccct gcccatcaac cgcaaacaga aggtcttccc
     3421 caacggaacg ctgattatcg agaatgtgga acggaactca gaccaagcga cctacacttg
     3481 cgttgccaag aatcaggaag gatactctgc tcgaggatct ctggaagtgc aagtcatggt
     3541 tcctcctaaa atcactccct tcaccttcgg cgatgaacct accaatgtgg aggattcggt
     3601 ttcggtgacc tgcttgatct ccagcggaga cctacccatc gacatagagt ggctatttaa
     3661 tgactacggg attagctcct tctccgggat gaccgtttat agaggaggaa agagaacgag
     3721 catgctgacc atagacaatg tccatgctcg ccacgcgggc aaatatagtt gtcgagcgag
     3781 aaatcatgcg tctgcggtta atttcacaac cgagttgatt gtgaatgtac ctcccagatg
     3841 gatcctcgag cccaccgata aggccttcgc ccagggatcc gatgccaagg ttgaatgcaa
     3901 ggctgatggc ttccccaaac cccaagttac atggaagaaa gcagttggcg acacccccgg
     3961 agagtacaag gatctaaaga agagcgacaa catccgcgtg gaagagggca ctctgcatgt
     4021 cgacaacatc caaaagacca acgagggcta ctatctgtgc gaggctatca atggaatagg
     4081 ctccggcctc tcggctgtga ttatgatcag cgttcaggcc cctccagagt tcacggagaa
     4141 gctgcgcaac cagaccgccc gacgaggaga acccgccgta ctccagtgcg aggcgaaggg
     4201 cgagaagccc attggcatct tgtggaacat gaacaacatg cgactggacc caaagaacga
     4261 caatcggtac accattcgtg aggagatcct ttccaccgga gttatgtcca gtctgtcgat
     4321 caagcgcacc gagagatccg actccgccct attcacctgt gtcgccacta atgcctttgg
     4381 atccgatgac gccagcataa atatgattgt ccaggaagtt ccggagatgc catatgcttt
     4441 gaaggtactc gacaaatccg gacgttccgt gcagctgagc tgggcgcaac cctacgatgg
     4501 caactcccct ctggacaggt acatcattga gtttaagcgt tctcgtgcct cctggagcga
     4561 aattgatcgc gtcatcgtgc ccggacacac cacagaagcc caagtgcaga agctcagccc
     4621 cgccaccacc tacaacattc gcatcgtggc cgaaaacgcc atcggcacat cccaatcctc
     4681 tgaagcggtc actatcatca ctagtggatc ttccggaatc atcccagttg aggaggagaa
     4741 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     4801 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     4861 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     4921 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     4981 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     5041 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     5101 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     5161 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     5221 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     5281 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
     5341 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     5401 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     5461 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     5521 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     5581 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     5641 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     5701 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     5761 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     5821 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     5881 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     5941 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     6001 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     6061 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     6121 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     6181 gggtcatcat caccatcacc attaatctag agatcttatt aaagcagaac ttgtttattg
     6241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
     6361 cgactctagc tagactcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8281 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8341 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8401 gaaaacctct gacacatgca gctcccggag gaatgtgtgt cagttagggt gtggaaagtc
     8461 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8581 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8701 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8761 caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca cagcttgtct
     8821 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     8881 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     8941 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     9001 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9061 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9121 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9181 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9241 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9301 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9361 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9421 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     9481 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     9541 gattaagttg ggtaacgcca gggt
//
