LOCUS       sequence-232940-        4552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""Binary vector for Agrobacterium tumefacience-mediated 
            transformation of plant cells resulting in constitutive expression 
            of cytoplasmatically localized RS-GFP"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Nocarova E, Fischer L
  TITLE     Cloning of transgenic tobacco BY-2 cells; an efficient method to 
            analyse and reduce high natural heterogeneity of transgene 
            expression.
  JOURNAL   BMC Plant Biol. 2009 Apr 22;9:44. doi: 10.1186/1471-2229-9-44.
  PUBMED    19386122
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2009-04-22"; volume: "9:44. doi"; pages: " 
            10.1186/1471-2229-9-44"
COMMENT     Sequence Label: pCP60 RS-GFP
FEATURES             Location/Qualifiers
     source          1..4552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             385..1179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(439..458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1049..1068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1569..1821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2370..2391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2406..2411,2412..2429,2430..2436)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2444..2460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2449..2471
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2468..2484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2468..2484
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3008..3353
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3296..3316
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(3418..3446)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4041..4061
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      4144..4396
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4409..4426)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4409..4425)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4418..4440)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg tggcggctct
     1921 gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga aaagatggca
     1981 aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct
     2041 aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg tttcattggt
     2101 gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg ctctaattcc
     2161 caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt ccgtcaatat
     2221 ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggccc aatacgcaaa
     2281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     2341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcccc
     2521 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     2581 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     2641 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     2701 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     2761 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     2821 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     2881 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     2941 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     3001 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     3061 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     3121 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     3181 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     3241 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     3301 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     3361 ctctagagga tccaaggaga tataacaatg agtaaaggag aagaactttt cactggagtt
     3421 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     3481 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     3541 aaactacctg ttccatggcc aacacttgtc actactttca cttatggtgt tcaatgcttt
     3601 tcaagatacc cagatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga
     3661 tacgtgcagg agaggaccat ctctttcaag gacgacggga actacaagac acgtgctgaa
     3721 gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag
     3781 gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac
     3841 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattag acacaacatt
     3901 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     3961 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     4021 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     4081 ggcatggatg aactatacaa ataaggggta ccgcggccgc gaattcagag ctcgaatttc
     4141 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     4201 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     4261 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     4321 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     4381 tctatgttac tagatcggga attaattcac tggccgtcgt tttacaacgt cgtgactggg
     4441 aaaaccctgg cgttacccaa cttaatcgcc tttgcagcac atcccccttt cgccagctgg
     4501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cc
//
