LOCUS       sequence-232945-       12743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial genome editing plasmid with kanamycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
  TITLE     Expanding the potential of CRISPR-Cpf1 based genome editing 
            technology in the cyanobacterium Anabaena PCC 7120.
  JOURNAL   ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
  PUBMED    30525474
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437."
COMMENT     Sequence Label: pCpf1
FEATURES             Location/Qualifiers
     source          1..12743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      496..890
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1232..1319
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2558..3529
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             4040..4879
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4866..5717
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     6227..6246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(6350..6355,6356..6373,6374..6379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        10303..10337
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(10405..10701)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     10551..10573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10565..10582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10566..10582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10592..10611
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10592..10610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10658..10678)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10658..10676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10697..10713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10697..10713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10710..10732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10721..10737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10745..10751,10752..10769,10770..10775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10790..10811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(11566..12381)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11566..12381)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12289..12308
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12373..12392
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tcattaaacg cgtattcagg ctgaccctgc gcgctgcgca gggctttatt gattccattt
       61 ttacactgat gaatgttccg ttgcgctgcc cggattacag ctgaaagcga ccaggtgctc
      121 ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag
      181 aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag
      241 cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg
      301 gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg
      361 catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc
      421 ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc
      481 ccgtactaac tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac
      541 gaacccctgc aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg
      601 cggggtgttg gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc
      661 agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct
      721 ggtctattcg ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg
      781 ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc
      841 gctttagcgg cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc
      901 tgtctcggtc gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg
      961 cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc
     1021 actcgctgct gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt
     1081 cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc
     1141 gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca
     1201 tcttgacacc ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc
     1261 aatcccgacc ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc
     1321 ctaacggcca cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca
     1381 aaagcaacag cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc
     1441 gggcggccaa tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg
     1501 cgacatggat gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc
     1561 cgccgactac tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga
     1621 ggtcgaattt gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga
     1681 gttcgcccag cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg
     1741 tggcggcgag aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga
     1801 gcggcccgcc gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc
     1861 acagaagacc gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc
     1921 cgaccatgcc aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac
     1981 acttgaggcg cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga
     2041 gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc
     2101 caacgcccag atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg
     2161 acagagtgaa gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc
     2221 agagcatggc gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca
     2281 gcgaggcgca ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg
     2341 agctggccaa agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc
     2401 ggagcgcgtc gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga
     2461 tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa
     2521 ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca
     2581 ggccataggc cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga
     2641 cgccaccacc ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac
     2701 gccatggctc aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca
     2761 ggagcggcat ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa
     2821 agccgagggc cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg
     2881 ggtcaaggtg gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc
     2941 cagcgagtac gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg
     3001 cttcaccaac cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct
     3061 gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca
     3121 gcagatcgag caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact
     3181 gcccgagcgg cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat
     3241 ggccgggctg gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca
     3301 gaagctggcc agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag
     3361 cccagcgctg gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag
     3421 caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc
     3481 accccgccag cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg
     3541 gtactcacgc ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga
     3601 aaaaagcgct tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg
     3661 tggctttggt tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc
     3721 cagccttaac cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc
     3781 cggcaagttt ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc
     3841 tggccagccc tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga
     3901 tgcgtcagcg caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg
     3961 aagaactcag agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg
     4021 cctgcaaagg aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc
     4081 agcagcgccg ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc
     4141 gctggtgtcg cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat
     4201 tgcaggcggg ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct
     4261 gcccgccgaa gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct
     4321 cagcgccgag gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag
     4381 cctgcccaac atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg
     4441 ccgcctgatg gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg
     4501 ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat
     4561 cgtgttcctg caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca
     4621 ggccagccgg ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag
     4681 catgaccagc gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt
     4741 ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg
     4801 gcatgacggc ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt
     4861 gccccgtggt gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc
     4921 actgtctggc ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc
     4981 tggacgtgac gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc
     5041 tgggcgctga tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc
     5101 tagtgcttgg cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac
     5161 ccaagtggga ggccgtcacc gctgatgcca tggtggtcaa aggtagctat cgggcgctgg
     5221 caaaggaaat cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca
     5281 tcgagcgcct ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc
     5341 ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc
     5401 ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg
     5461 tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg
     5521 accccggcaa aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag
     5581 aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc
     5641 tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc
     5701 ccaaggcggc aggctgaccc cccccactct attgtaaaca agacattttt atcttttata
     5761 ttcaatggct tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag
     5821 gcttaacaat attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct
     5881 ttcctggctt tgcttccaga tgtatgctct tctgctcctg caggcatcgt ggtgtcacgc
     5941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     6241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcact ttacacttta
     6361 tgcttccggc tcgtatgtta ggaggtcttt atcatgtcaa tttatcaaga atttgttaat
     6421 aaatatagtt taagtaaaac tctaagattt gagttaatcc cacagggtaa aacacttgaa
     6481 aacataaaag caagaggttt gattttagat gatgagaaaa gagctaaaga ctacaaaaag
     6541 gctaaacaaa taattgataa atatcatcag ttttttatag aggagatatt aagttcggtt
     6601 tgtattagcg aagatttatt acaaaactat tctgatgttt attttaaact taaaaagagt
     6661 gatgatgata atctacaaaa agattttaaa agtgcaaaag atacgataaa gaaacaaata
     6721 tctgaatata taaaggactc agagaaattt aagaatttgt ttaatcaaaa ccttatcgat
     6781 gctaaaaaag ggcaagagtc agatttaatt ctatggctaa agcaatctaa ggataatggt
     6841 atagaactat ttaaagccaa tagtgatatc acagatatag atgaggcgtt agaaataatc
     6901 aaatctttta aaggttggac aacttatttt aagggttttc atgaaaatag aaaaaatgtt
     6961 tatagtagca atgatattcc tacatctatt atttatagga tagtagatga taatttgcct
     7021 aaatttctag aaaataaagc taagtatgag agtttaaaag acaaagctcc agaagctata
     7081 aactatgaac aaattaaaaa agatttggca gaagagctaa cctttgatat tgactacaaa
     7141 acatctgaag ttaatcaaag agttttttca cttgatgaag tttttgagat agcaaacttt
     7201 aataattatc taaatcaaag tggtattact aaatttaata ctattattgg tggtaaattt
     7261 gtaaatggtg aaaatacaaa gagaaaaggt ataaatgaat atataaatct atactcacag
     7321 caaataaatg ataaaacact caaaaaatat aaaatgagtg ttttatttaa gcaaatttta
     7381 agtgatacag aatctaaatc ttttgtaatt gataagttag aagatgatag tgatgtagtt
     7441 acaacgatgc aaagttttta tgagcaaata gcagctttta aaacagtaga agaaaaatct
     7501 attaaagaaa cactatcttt attatttgat gatttaaaag ctcaaaaact tgatttgagt
     7561 aaaatttatt ttaaaaatga taaatctctt actgatctat cacaacaagt ttttgatgat
     7621 tatagtgtta ttggtacagc ggtactagaa tatataactc aacaaatagc acctaaaaat
     7681 cttgataacc ctagtaagaa agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa
     7741 tacttatctc tagaaactat aaagcttgcc ttagaagaat ttaataagca tagagatata
     7801 gataaacagt gtaggtttga agaaatactt gcaaactttg cggctattcc gatgatattt
     7861 gatgaaatag ctcaaaacaa agacaatttg gcacagatat ctatcaaata tcaaaatcaa
     7921 ggtaaaaaag acctacttca agctagtgcg gaagatgatg ttaaagctat caaggatctt
     7981 ttagatcaaa ctaataatct cttacataaa ctaaaaatat ttcatattag tcagtcagaa
     8041 gataaggcaa atattttaga caaggatgag catttttatc tagtatttga ggagtgctac
     8101 tttgagctag cgaatatagt gcctctttat aacaaaatta gaaactatat aactcaaaag
     8161 ccatatagtg atgagaaatt taagctcaat tttgagaact cgactttggc taatggttgg
     8221 gataaaaata aagagcctga caatacggca attttattta tcaaagatga taaatattat
     8281 ctgggtgtga tgaataagaa aaataacaaa atatttgatg ataaagctat caaagaaaat
     8341 aaaggcgagg gttataaaaa aattgtttat aaacttttac ctggcgcaaa taaaatgtta
     8401 cctaaggttt tcttttctgc taaatctata aaattttata atcctagtga agatatactt
     8461 agaataagaa atcattccac acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa
     8521 tttgagttta atattgaaga ttgccgaaaa tttatagatt tttataaaca gtctataagt
     8581 aagcatccgg agtggaaaga ttttggattt agattttctg atactcaaag atataattct
     8641 atagatgaat tttatagaga agttgaaaat caaggctaca aactaacttt tgaaaatata
     8701 tcagagagct atattgatag cgtagttaat cagggtaaat tgtacctatt ccaaatctat
     8761 aataaagatt tttcagctta tagcaaaggg cgaccaaatc tacatacttt atattggaaa
     8821 gcgctgtttg atgagagaaa tcttcaagat gtggtttata agctaaatgg tgaggcagag
     8881 cttttttatc gtaaacaatc aatacctaaa aaaatcactc acccagctaa agaggcaata
     8941 gctaataaaa acaaagataa tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa
     9001 gataaacgct ttactgaaga taagtttttc tttcactgtc ctattacaat caattttaaa
     9061 tctagtggag ctaataagtt taatgatgaa atcaatttat tgctaaaaga aaaagcaaat
     9121 gatgttcata tattaagtat agatagaggt gaaagacatt tagcttacta tactttggta
     9181 gatggtaaag gcaatatcat caaacaagat actttcaaca tcattggtaa tgatagaatg
     9241 aaaacaaact accatgataa gcttgctgca atagagaaag atagggattc agctaggaaa
     9301 gactggaaaa agataaataa catcaaagag atgaaagagg gctatctatc tcaggtagtt
     9361 catgaaatag ctaagctagt tatagagtat aatgctattg tggtttttga ggatttaaat
     9421 tttggattta aaagagggcg tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa
     9481 atgctaattg agaaactaaa ctatctagtt ttcaaagata atgagtttga taaaactggg
     9541 ggagtgctta gagcttatca gctaacagca ccttttgaga cttttaaaaa gatgggtaaa
     9601 caaacaggta ttatctacta tgtaccagct ggttttactt caaaaatttg tcctgtaact
     9661 ggttttgtaa atcagttata tcctaagtat gaaagtgtca gcaaatctca agagttcttt
     9721 agtaagtttg acaagatttg ttataacctt gataagggct attttgagtt tagttttgat
     9781 tataaaaact ttggtgacaa ggctgccaaa ggcaagtgga ctatagctag ctttgggagt
     9841 agattgatta actttagaaa ttcagataaa aatcataatt gggatactcg agaagtttat
     9901 ccaactaaag agttggagaa attgctaaaa gattattcta tcgaatatgg gcatggcgaa
     9961 tgtatcaaag cagctatttg cggtgagagc gacaaaaagt tttttgctaa gctaactagt
    10021 gtcctaaata ctatcttaca aatgcgtaac tcaaaaacag gtactgagtt agattatcta
    10081 atttcaccag tagcagatgt aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat
    10141 atgcctcaag atgctgatgc caatggtgct tatcatattg ggctaaaagg tctgatgcta
    10201 ctaggtagga tcaaaaataa tcaagagggc aaaaaactca atttggttat caaaaatgaa
    10261 gagtattttg agttcgtgca gaataggaat aactaagaat tcttgacagc tagctcagtc
    10321 ctaggtataa tgctagcgct gatttaggca aaaacgggtc taagaacttt aaataatttc
    10381 tactgttgta gatgagagca ggtgtcagcc gctacagggc gcgtcccatt cgccattcag
    10441 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10501 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    10561 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
    10621 taccgggccc cccctcgagg tcctccagct tttgttccct ttagtgaggg ttaattgcgc
    10681 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10741 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10801 aactcacatt aattgcgttg cgctcactgc ccgctttcca cccacctgca aaagtctaag
    10861 aactttaaat aatttctact gttgtagatg ctactattcc tgtgccttca gataattcag
    10921 tctaagaact ttaaataatt tctactgttg tagatgtcta gagccttttg tattagtagc
    10981 cggtctaaga actttaaata atttctactg ttgtagatta gcgatttatg aaggtcattt
    11041 ttttgtctag ctttaatgcg gtagttggta ccgtcgatgg cagaaattcg atatctagat
    11101 ctcatggatc cgcaatggca acaacgttgc gctcgactct agaggatctg taatccgggc
    11161 agcgcaacgg aacattcatc agtgtaaaaa tggaatcaat aaagccctgc gcagcgcgca
    11221 gggtcagcct gaatacgcgt ttaatgacca gcacagtcgt gatggcaagg tcagaatagc
    11281 gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat
    11341 catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt
    11401 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga
    11461 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt
    11521 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
    11581 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
    11641 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
    11701 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
    11761 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
    11821 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
    11881 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
    11941 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
    12001 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
    12061 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
    12121 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
    12181 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
    12241 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
    12301 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
    12361 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
    12421 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
    12481 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
    12541 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
    12601 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
    12661 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gctattctga
    12721 ccttgccatc acgactgtgc tgg
//
