LOCUS       sequence-232946-       12595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial genome editing plasmid with spectinomycin resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
  TITLE     Expanding the potential of CRISPR-Cpf1 based genome editing 
            technology in the cyanobacterium Anabaena PCC 7120.
  JOURNAL   ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
  PUBMED    30525474
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437."
COMMENT     Sequence Label: pCpf1-sp
FEATURES             Location/Qualifiers
     source          1..12595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            232..319
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             1558..2529
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3040..3879
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             3866..4717
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     5227..5246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(5350..5355,5356..5373,5374..5379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        9303..9337
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(9405..9701)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     9551..9573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9565..9582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9566..9582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9592..9611
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9592..9610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9658..9678)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9658..9676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9697..9713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9697..9713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9710..9732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9721..9737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9745..9751,9752..9769,9770..9775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9790..9811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             10521..11312
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      12091..12485
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 catgagccga tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac
       61 cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg
      121 ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc
      181 cttgttgctc cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat
      241 catggaggca cagcggcggc aatcccgacc ctactttgta ggggagggcg cacttaccgg
      301 tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct ccgagggcca
      361 ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg
      421 gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga ctacatccag
      481 cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg
      541 ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc
      601 aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag
      661 cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat
      721 acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag
      781 cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag
      841 accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc atggcttgag
      901 cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac
      961 gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac
     1021 ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg
     1081 gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca
     1141 atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga
     1201 gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat
     1261 gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca
     1321 gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag
     1381 cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac
     1441 cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct
     1501 cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg
     1561 aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc
     1621 ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc
     1681 gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg
     1741 tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag
     1801 tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc
     1861 ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg
     1921 cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc
     1981 cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt
     2041 tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg
     2101 ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc
     2161 aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg
     2221 gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag
     2281 tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc
     2341 aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat
     2401 tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc
     2461 gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc
     2521 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
     2581 gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac
     2641 aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc
     2701 ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc
     2761 gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga
     2821 caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga
     2881 gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga
     2941 ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc
     3001 cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc
     3061 aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg
     3121 gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc
     3181 ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc
     3241 accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg
     3301 cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg
     3361 atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc
     3421 aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc
     3481 gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc
     3541 gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg
     3601 ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc
     3661 tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac
     3721 gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc
     3781 gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg
     3841 cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca
     3901 cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg
     3961 cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggcgacggca agcggatcga
     4021 gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc
     4081 catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca
     4141 gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgatgcca tggtggtcaa
     4201 aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct
     4261 caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg
     4321 ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg
     4381 cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt
     4441 gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca
     4501 gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag ataccttgtg
     4561 cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt
     4621 gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg
     4681 caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca
     4741 agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa
     4801 aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc
     4861 gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct tctgctcctg
     4921 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4981 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5041 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5101 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5161 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5221 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5281 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5341 ctcgtgcact ttacacttta tgcttccggc tcgtatgtta ggaggtcttt atcatgtcaa
     5401 tttatcaaga atttgttaat aaatatagtt taagtaaaac tctaagattt gagttaatcc
     5461 cacagggtaa aacacttgaa aacataaaag caagaggttt gattttagat gatgagaaaa
     5521 gagctaaaga ctacaaaaag gctaaacaaa taattgataa atatcatcag ttttttatag
     5581 aggagatatt aagttcggtt tgtattagcg aagatttatt acaaaactat tctgatgttt
     5641 attttaaact taaaaagagt gatgatgata atctacaaaa agattttaaa agtgcaaaag
     5701 atacgataaa gaaacaaata tctgaatata taaaggactc agagaaattt aagaatttgt
     5761 ttaatcaaaa ccttatcgat gctaaaaaag ggcaagagtc agatttaatt ctatggctaa
     5821 agcaatctaa ggataatggt atagaactat ttaaagccaa tagtgatatc acagatatag
     5881 atgaggcgtt agaaataatc aaatctttta aaggttggac aacttatttt aagggttttc
     5941 atgaaaatag aaaaaatgtt tatagtagca atgatattcc tacatctatt atttatagga
     6001 tagtagatga taatttgcct aaatttctag aaaataaagc taagtatgag agtttaaaag
     6061 acaaagctcc agaagctata aactatgaac aaattaaaaa agatttggca gaagagctaa
     6121 cctttgatat tgactacaaa acatctgaag ttaatcaaag agttttttca cttgatgaag
     6181 tttttgagat agcaaacttt aataattatc taaatcaaag tggtattact aaatttaata
     6241 ctattattgg tggtaaattt gtaaatggtg aaaatacaaa gagaaaaggt ataaatgaat
     6301 atataaatct atactcacag caaataaatg ataaaacact caaaaaatat aaaatgagtg
     6361 ttttatttaa gcaaatttta agtgatacag aatctaaatc ttttgtaatt gataagttag
     6421 aagatgatag tgatgtagtt acaacgatgc aaagttttta tgagcaaata gcagctttta
     6481 aaacagtaga agaaaaatct attaaagaaa cactatcttt attatttgat gatttaaaag
     6541 ctcaaaaact tgatttgagt aaaatttatt ttaaaaatga taaatctctt actgatctat
     6601 cacaacaagt ttttgatgat tatagtgtta ttggtacagc ggtactagaa tatataactc
     6661 aacaaatagc acctaaaaat cttgataacc ctagtaagaa agagcaagaa ttaatagcca
     6721 aaaaaactga aaaagcaaaa tacttatctc tagaaactat aaagcttgcc ttagaagaat
     6781 ttaataagca tagagatata gataaacagt gtaggtttga agaaatactt gcaaactttg
     6841 cggctattcc gatgatattt gatgaaatag ctcaaaacaa agacaatttg gcacagatat
     6901 ctatcaaata tcaaaatcaa ggtaaaaaag acctacttca agctagtgcg gaagatgatg
     6961 ttaaagctat caaggatctt ttagatcaaa ctaataatct cttacataaa ctaaaaatat
     7021 ttcatattag tcagtcagaa gataaggcaa atattttaga caaggatgag catttttatc
     7081 tagtatttga ggagtgctac tttgagctag cgaatatagt gcctctttat aacaaaatta
     7141 gaaactatat aactcaaaag ccatatagtg atgagaaatt taagctcaat tttgagaact
     7201 cgactttggc taatggttgg gataaaaata aagagcctga caatacggca attttattta
     7261 tcaaagatga taaatattat ctgggtgtga tgaataagaa aaataacaaa atatttgatg
     7321 ataaagctat caaagaaaat aaaggcgagg gttataaaaa aattgtttat aaacttttac
     7381 ctggcgcaaa taaaatgtta cctaaggttt tcttttctgc taaatctata aaattttata
     7441 atcctagtga agatatactt agaataagaa atcattccac acatacaaaa aatggtagtc
     7501 ctcaaaaagg atatgaaaaa tttgagttta atattgaaga ttgccgaaaa tttatagatt
     7561 tttataaaca gtctataagt aagcatccgg agtggaaaga ttttggattt agattttctg
     7621 atactcaaag atataattct atagatgaat tttatagaga agttgaaaat caaggctaca
     7681 aactaacttt tgaaaatata tcagagagct atattgatag cgtagttaat cagggtaaat
     7741 tgtacctatt ccaaatctat aataaagatt tttcagctta tagcaaaggg cgaccaaatc
     7801 tacatacttt atattggaaa gcgctgtttg atgagagaaa tcttcaagat gtggtttata
     7861 agctaaatgg tgaggcagag cttttttatc gtaaacaatc aatacctaaa aaaatcactc
     7921 acccagctaa agaggcaata gctaataaaa acaaagataa tcctaaaaaa gagagtgttt
     7981 ttgaatatga tttaatcaaa gataaacgct ttactgaaga taagtttttc tttcactgtc
     8041 ctattacaat caattttaaa tctagtggag ctaataagtt taatgatgaa atcaatttat
     8101 tgctaaaaga aaaagcaaat gatgttcata tattaagtat agatagaggt gaaagacatt
     8161 tagcttacta tactttggta gatggtaaag gcaatatcat caaacaagat actttcaaca
     8221 tcattggtaa tgatagaatg aaaacaaact accatgataa gcttgctgca atagagaaag
     8281 atagggattc agctaggaaa gactggaaaa agataaataa catcaaagag atgaaagagg
     8341 gctatctatc tcaggtagtt catgaaatag ctaagctagt tatagagtat aatgctattg
     8401 tggtttttga ggatttaaat tttggattta aaagagggcg tttcaaggta gagaagcagg
     8461 tctatcaaaa gttagaaaaa atgctaattg agaaactaaa ctatctagtt ttcaaagata
     8521 atgagtttga taaaactggg ggagtgctta gagcttatca gctaacagca ccttttgaga
     8581 cttttaaaaa gatgggtaaa caaacaggta ttatctacta tgtaccagct ggttttactt
     8641 caaaaatttg tcctgtaact ggttttgtaa atcagttata tcctaagtat gaaagtgtca
     8701 gcaaatctca agagttcttt agtaagtttg acaagatttg ttataacctt gataagggct
     8761 attttgagtt tagttttgat tataaaaact ttggtgacaa ggctgccaaa ggcaagtgga
     8821 ctatagctag ctttgggagt agattgatta actttagaaa ttcagataaa aatcataatt
     8881 gggatactcg agaagtttat ccaactaaag agttggagaa attgctaaaa gattattcta
     8941 tcgaatatgg gcatggcgaa tgtatcaaag cagctatttg cggtgagagc gacaaaaagt
     9001 tttttgctaa gctaactagt gtcctaaata ctatcttaca aatgcgtaac tcaaaaacag
     9061 gtactgagtt agattatcta atttcaccag tagcagatgt aaatggcaat ttctttgatt
     9121 cgcgacaggc gccaaaaaat atgcctcaag atgctgatgc caatggtgct tatcatattg
     9181 ggctaaaagg tctgatgcta ctaggtagga tcaaaaataa tcaagagggc aaaaaactca
     9241 atttggttat caaaaatgaa gagtattttg agttcgtgca gaataggaat aactaagaat
     9301 tcttgacagc tagctcagtc ctaggtataa tgctagcgct gatttaggca aaaacgggtc
     9361 taagaacttt aaataatttc tactgttgta gatgagagca ggtgtcagcc gctacagggc
     9421 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     9481 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     9541 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     9601 tcactatagg gcgaattggg taccgggccc cccctcgagg tcctccagct tttgttccct
     9661 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9841 cccacctgca aaagtctaag aactttaaat aatttctact gttgtagatg ctactattcc
     9901 tgtgccttca gataattcag tctaagaact ttaaataatt tctactgttg tagatgtcta
     9961 gagccttttg tattagtagc cggtctaaga actttaaata atttctactg ttgtagatta
    10021 gcgatttatg aaggtcattt ttttgtctag ctttaatgcg gtagttggta ccgtcgatgg
    10081 cagaaattcg atatctagat ctcatggatc cgcaatggca acaacgttgc gctcgactct
    10141 agaagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc
    10201 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
    10261 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
    10321 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
    10381 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
    10441 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
    10501 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
    10561 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
    10621 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
    10681 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
    10741 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
    10801 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
    10861 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
    10921 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
    10981 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
    11041 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
    11101 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
    11161 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
    11221 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
    11281 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
    11341 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
    11401 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
    11461 attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta
    11521 atcgcaacat ccgcattaaa atctagcgag ggctttacta agctgatccg gtggatgacc
    11581 ttttgaatga cctttaatag attatattac taattaattg gggaccctag aggtcccctt
    11641 ttttatttta aaaatttttt cacaaaacgg tttacaagca taaagcttgc tcaatctgaa
    11701 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
    11761 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
    11821 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
    11881 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
    11941 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
    12001 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
    12061 cagtgcccgt cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc
    12121 tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc
    12181 aggggcgggg gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca
    12241 ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc
    12301 agaggccgga tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg
    12361 gcagggggaa aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct
    12421 ggagcgcttt tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg
    12481 cagccccgca gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg
    12541 gcagaccgaa caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgc
//
