LOCUS       sequence-232950-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase 
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.25 EC10-AP-His
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..40
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     32..49
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     33..49
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        79..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     663..687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          802..1627
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6199..6216
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6242..6363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6279..6298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6333..6352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6401..6418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6572..7160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6652..6671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7331..8122,8123..8191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7954..7973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8192..8296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8364..8382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8420..8442)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8444..8464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8449..8778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8629..8764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8691..8710
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8915..8934
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9001..9456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9138..9159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9350..9369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtcgag
       61 gtctcgacgg tatcgattga cattgattat tgactagcta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      601 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      781 attccccgtg ccaagagtga cgtaagtacc gcctatagac tctataggca cacccctttg
      841 gctcttatgc atgctatact gtttttggct tggggcctat acacccccgc ttccatatgc
      901 tataggtgat ggtatagctt agcctatagg tgtgggttat tgaccattat tgaccactcc
      961 cctattggtg acgatacttt ccattactaa tccataacat ggctctttgc cacaactatc
     1021 tctattggct atatgccaat actctgtcct tcagagactg acacggactc tgtattttta
     1081 caggatgggg tcccatttat tatttacaaa ttcacatata caacaacgcc gtcccccgtg
     1141 cccgcagttt ttattaaaca tagcgtggga tctccacgcg aatctcgggt acgtgttccg
     1201 gacatgggct cttctccggt agcggcggag cttccacatc cgagccctgg tcccatgcct
     1261 ccagcggctc atggtcgctc ggcagctcct tgctcctaac agtggaggcc agacttaggc
     1321 acagcacaat gcccaccacc accagtgtgc cgcacaaggc cgtggcggta gggtatgtgt
     1381 ctgaaaatga gctcggagat tgggctcgca ccgctgacgc agatggaaga cttaaggcag
     1441 cggcagaaga agatgcaggc agctgagttg ttgtattctg ataagagtca gaggtaactc
     1501 ccgttgcggt gctgttaacg gtggagggca gtgtagtctg agcagtactc gttgctgccg
     1561 cgcgcgccac cagacataat agctgacaga ctaacagact gttcctttcc atgggtcttt
     1621 tctgcagaag cttgaattct cgaggatccg cggccgctac tgccaacttc acacaacccg
     1681 ctcagcggaa acacggcata cgaaatgaat atgcccaacg aacgcctcaa atggctaatg
     1741 ctgttcgcag ctgttgccct cattgcttgt ggtagtcaga ccctagctgc caatccccca
     1801 gatgccgacc aaaaaggacc cgtcttcctc aaggaaccca ccaaccgcat tgacttctcc
     1861 aactccacgg gcgcagagat cgagtgcaag gccagcggca atcccatgcc cgagattatt
     1921 tggatcagga gcgacggtac cgccgtgggt gatgtgcccg gattgcgtca gatctcatcc
     1981 gacggcaagc tggtcttccc tcccttccgc gccgaggact accgccagga ggtccatgcc
     2041 caggtgtacg cctgcctggc ccgcaaccag ttcggatcca ttatctcccg ggacgtccat
     2101 gtgcgagccg ttgtcaatca gttctatgag gcggagatta tgaccgagta tgttatcagg
     2161 ggtaatgctg ccgttctgaa gtgttccatc ccgtcgttcg tggctgattt tgttcgagtt
     2221 gagtcctgga tcgatgatga gggcaatgtc ttgtcctttt cggacaatta cgatggaaag
     2281 tatctggtat tgccctctgg agagctgcac atccgtgagg ttggacccga ggacggatac
     2341 aagagctacc agtgccgaac caaacatcgt ctgaccggag aaacccgatt aagtgccaca
     2401 aaaggacgat tggtcatcac agaacctgtg ggcagtgttg cgcctaagat cagcgtagaa
     2461 aatcgtctga aaaatgcaga ggctcgaatt ggttccacct tcaccctctt ttgccctggt
     2521 caagcctatc cagtaccctt ctttagatgg tacaagttca tcgaaggaac aacccgtaaa
     2581 caagctgtgg tgctaaatga ccgtgtgaag caggtctctg gtactctcat tattaaggac
     2641 gcagtagtcg aggactccgg aaaatacctg tgcgtggtga acaattccgt gggaggcgaa
     2701 agcgttgaga ctgtgcttac cgtcactgcc cccctatccg ctaagatcga tcctcccaca
     2761 cagacagttg acttcggacg ccccgccgta ttcacttgcc agtacactgg aaatcccatc
     2821 aagaccgtta gctggatgaa ggacggcaag gctatcggac acagcgaacc cgtgctgcgc
     2881 atcgaatccg tgaagaagga ggacaagggc atgtaccagt gcttcgtaag aaacgaccag
     2941 gaatcggcgg aggcgagtgc tgagctgaag ctcggaggcc gtttcgatcc ccccgtcatc
     3001 cgacaggcct tccaggaaga gaccatggaa cccggaccaa gtgtattcct caagtgcgtc
     3061 gccggcggaa accccacgcc cgaaatttcc tgggaactcg acggcaagaa aatcgccaac
     3121 aacgatcgct atcaggtcgg acagtatgtg acggtcaacg gagatgtggt ttcctatctg
     3181 aacataacct cggtccacgc caacgatggc ggtctctaca agtgcatagc caagagcaaa
     3241 gtgggcgtgg ccgaacactc ggccaagttg aatgtatatg gactgcccta catccggcaa
     3301 atggagaaga aggcaattgt tgctggagaa accctgattg tcacatgtcc tgtagctgga
     3361 taccccatcg attcgattgt ctgggagcga gataaccgtg ccctgcccat caaccgcaaa
     3421 cagaaggtct tccccaacgg aacgctgatt atcgagaatg tggaacggaa ctcagaccaa
     3481 gcgacctaca cttgcgttgc caagaatcag gaaggatact ctgctcgagg atctctggaa
     3541 gtgcaagtca tggttccacc caaaattacg cccttcgact tcggtgacgc tccgacgaat
     3601 gtggaggact ctgtttcggt catgtgcctg atttccaatg gggatttacc catcgacatc
     3661 gagtggttct ttaacgacta cggaattagt tcgtactccg gaataaatgt agttaaaggt
     3721 ggcaagagga acagcatgct aagtattgat agtgtccagg ctcgtcatgc cgggaagtat
     3781 agttgtcggg ctaagaacca cgctgctgct gtaaattata ccaccgaact aatagttaat
     3841 gtacctccca gatggatcct cgagcccacc gataaggcct tcgcccaggg atccgatgcc
     3901 aaggttgaat gcaaggctga tggcttcccc aaaccccaag ttacatggaa gaaagcagtt
     3961 ggcgacaccc ccggagagta caaggatcta aagaagagcg acaacatccg cgtggaagag
     4021 ggcactctgc atgtcgacaa catccaaaag accaacgagg gctactatct gtgcgaggct
     4081 atcaatggaa taggctccgg cctctcggct gtgattatga tcagcgttca ggcccctcca
     4141 gagttcacgg agaagctgcg caaccagacc gcccgacgag gagaacccgc cgtactccag
     4201 tgcgaggcga agggcgagaa gcccattggc atcttgtgga acatgaacaa catgcgactg
     4261 gacccaaaga acgacaatcg gtacaccatt cgtgaggaga tcctttccac cggagttatg
     4321 tccagtctgt cgatcaagcg caccgagaga tccgactccg ccctattcac ctgtgtcgcc
     4381 actaatgcct ttggatccga tgacgccagc ataaatatga ttgtccagga agttccggag
     4441 atgccatatg ctttgaaggt actcgacaaa tccggacgtt ccgtgcagct gagctgggcg
     4501 caaccctacg atggcaactc ccctctggac aggtacatca ttgagtttaa gcgttctcgt
     4561 gcctcctgga gcgaaattga tcgcgtcatc gtgcccggac acaccacaga agcccaagtg
     4621 cagaagctca gccccgccac cacctacaac attcgcatcg tggccgaaaa cgccatcggc
     4681 acatcccaat cctctgaagc ggtcactatc atcactagtg gatcttccgg aatcatccca
     4741 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
     4801 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
     4861 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
     4921 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
     4981 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
     5041 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
     5101 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
     5161 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
     5221 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
     5281 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
     5341 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
     5401 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
     5461 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
     5521 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
     5581 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
     5641 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
     5701 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
     5761 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
     5821 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
     5881 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
     5941 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
     6001 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
     6061 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
     6121 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
     6181 gacgccgcgc acccgggtca tcatcaccat caccattaat ctagagatct tattaaagca
     6241 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6361 ttatcatgtc tggtcgactc tagctagact cttccgcttc ctcgctcact gactcgctgc
     6421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7621 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7921 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     8401 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaggaatgt gtgtcagtta
     8461 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8521 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8581 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8641 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8701 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8761 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgt cccggagacg
     8821 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8881 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8941 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     9001 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     9061 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     9121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     9181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     9241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     9301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     9361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     9421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     9481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9541 atgtgctgca aggcgattaa gttg
//
