LOCUS       sequence-232951-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase 
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.26 EC10-AP-His
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     589..613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          728..1553
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6125..6142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6168..6289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6205..6224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6259..6278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6327..6344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6498..7086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6578..6597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7257..8048,8049..8117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7880..7899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8118..8222)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8290..8308
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8346..8368)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8370..8390)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8375..8704
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8555..8690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8617..8636
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8841..8860
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8927..9382)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9064..9085)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9276..9295
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9508..9530
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9522..9539
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9523..9539
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gattgacatt gattattgac tagctagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      661 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      721 gagtgacgta agtaccgcct atagactcta taggcacacc cctttggctc ttatgcatgc
      781 tatactgttt ttggcttggg gcctatacac ccccgcttcc atatgctata ggtgatggta
      841 tagcttagcc tataggtgtg ggttattgac cattattgac cactccccta ttggtgacga
      901 tactttccat tactaatcca taacatggct ctttgccaca actatctcta ttggctatat
      961 gccaatactc tgtccttcag agactgacac ggactctgta tttttacagg atggggtccc
     1021 atttattatt tacaaattca catatacaac aacgccgtcc cccgtgcccg cagtttttat
     1081 taaacatagc gtgggatctc cacgcgaatc tcgggtacgt gttccggaca tgggctcttc
     1141 tccggtagcg gcggagcttc cacatccgag ccctggtccc atgcctccag cggctcatgg
     1201 tcgctcggca gctccttgct cctaacagtg gaggccagac ttaggcacag cacaatgccc
     1261 accaccacca gtgtgccgca caaggccgtg gcggtagggt atgtgtctga aaatgagctc
     1321 ggagattggg ctcgcaccgc tgacgcagat ggaagactta aggcagcggc agaagaagat
     1381 gcaggcagct gagttgttgt attctgataa gagtcagagg taactcccgt tgcggtgctg
     1441 ttaacggtgg agggcagtgt agtctgagca gtactcgttg ctgccgcgcg cgccaccaga
     1501 cataatagct gacagactaa cagactgttc ctttccatgg gtcttttctg cagaagcttg
     1561 aattctcgag gatccgcggc cgctactgcc aacttcacac aacccgctca gcggaaacac
     1621 ggcatacgaa atgaatatgc ccaacgaacg cctcaaatgg ctaatgctgt tcgcagctgt
     1681 tgccctcatt gcttgtggta gtcagaccct agctgccaat cccccagatg ccgaccaaaa
     1741 aggacccgtc ttcctcaagg aacccaccaa ccgcattgac ttctccaact ccacgggcgc
     1801 agagatcgag tgcaaggcca gcggcaatcc catgcccgag attatttgga tcaggagcga
     1861 cggtaccgcc gtgggtgatg tgcccggatt gcgtcagatc tcatccgacg gcaagctggt
     1921 cttccctccc ttccgcgccg aggactaccg ccaggaggtc catgcccagg tgtacgcctg
     1981 cctggcccgc aaccagttcg gatccattat ctcccgggac gtccatgtgc gagccgttgt
     2041 caatcagttc tatgaggcgg agattatgac cgagtatgtt atcaggggta atgctgccgt
     2101 tctgaagtgt tccatcccgt cgttcgtggc tgattttgtt cgagttgagt cctggatcga
     2161 tgatgagggc aatgtcttgt ccttttcgga caattacgat ggaaagtatc tggtattgcc
     2221 ctctggagag ctgcacatcc gtgaggttgg acccgaggac ggatacaaga gctaccagtg
     2281 ccgaaccaaa catcgtctga ccggagaaac ccgattaagt gccacaaaag gacgattggt
     2341 catcacagaa cctgtgggca gtgttgcgcc taagatcagc gtagaaaatc gtctgaaaaa
     2401 tgcagaggct cgaattggtt ccaccttcac cctcttttgc cctggtcaag cctatccagt
     2461 acccttcttt agatggtaca agttcatcga aggaacaacc cgtaaacaag ctgtggtgct
     2521 aaatgaccgt gtgaagcagg tctctggtac tctcattatt aaggacgcag tagtcgagga
     2581 ctccggaaaa tacctgtgcg tggtgaacaa ttccgtggga ggcgaaagcg ttgagactgt
     2641 gcttaccgtc actgcccccc tatccgctaa gatcgatcct cccacacaga cagttgactt
     2701 cggacgcccc gccgtattca cttgccagta cactggaaat cccatcaaga ccgttagctg
     2761 gatgaaggac ggcaaggcta tcggacacag cgaacccgtg ctgcgcatcg aatccgtgaa
     2821 gaaggaggac aagggcatgt accagtgctt cgtaagaaac gaccaggaat cggcggaggc
     2881 gagtgctgag ctgaagctcg gaggccgttt cgatcccccc gtcatccgac aggccttcca
     2941 ggaagagacc atggaacccg gaccaagtgt attcctcaag tgcgtcgccg gcggaaaccc
     3001 cacgcccgaa atttcctggg aactcgacgg caagaaaatc gccaacaacg atcgctatca
     3061 ggtcggacag tatgtgacgg tcaacggaga tgtggtttcc tatctgaaca taacctcggt
     3121 ccacgccaac gatggcggtc tctacaagtg catagccaag agcaaagtgg gcgtggccga
     3181 acactcggcc aagttgaatg tatatggact gccctacatc cggcaaatgg agaagaaggc
     3241 aattgttgct ggagaaaccc tgattgtcac atgtcctgta gctggatacc ccatcgattc
     3301 gattgtctgg gagcgagata accgtgccct gcccatcaac cgcaaacaga aggtcttccc
     3361 caacggaacg ctgattatcg agaatgtgga acggaactca gaccaagcga cctacacttg
     3421 cgttgccaag aatcaggaag gatactctgc tcgaggatct ctggaagtgc aagtcatggt
     3481 tcctccgaaa atcacgccat ttgacttcgg tgccgaaccc accaatgttg aggattccgt
     3541 ttcggtgact tgtctaatct cctccggcga tttgcccatt gatatcgagt ggttcttcaa
     3601 cgactacgga attagctcct actccggtat aaatgtagtt aagggtggca agaggaacag
     3661 tatgctgagc atcgataatg tgcaggctag gcatgcggga aagtatagtt gccgggcaaa
     3721 gaactacgca gcagccgtca actacagcac tgaactcata gttaatgtac ctcccagatg
     3781 gatcctcgag cccaccgata aggccttcgc ccagggatcc gatgccaagg ttgaatgcaa
     3841 ggctgatggc ttccccaaac cccaagttac atggaagaaa gcagttggcg acacccccgg
     3901 agagtacaag gatctaaaga agagcgacaa catccgcgtg gaagagggca ctctgcatgt
     3961 cgacaacatc caaaagacca acgagggcta ctatctgtgc gaggctatca atggaatagg
     4021 ctccggcctc tcggctgtga ttatgatcag cgttcaggcc cctccagagt tcacggagaa
     4081 gctgcgcaac cagaccgccc gacgaggaga acccgccgta ctccagtgcg aggcgaaggg
     4141 cgagaagccc attggcatct tgtggaacat gaacaacatg cgactggacc caaagaacga
     4201 caatcggtac accattcgtg aggagatcct ttccaccgga gttatgtcca gtctgtcgat
     4261 caagcgcacc gagagatccg actccgccct attcacctgt gtcgccacta atgcctttgg
     4321 atccgatgac gccagcataa atatgattgt ccaggaagtt ccggagatgc catatgcttt
     4381 gaaggtactc gacaaatccg gacgttccgt gcagctgagc tgggcgcaac cctacgatgg
     4441 caactcccct ctggacaggt acatcattga gtttaagcgt tctcgtgcct cctggagcga
     4501 aattgatcgc gtcatcgtgc ccggacacac cacagaagcc caagtgcaga agctcagccc
     4561 cgccaccacc tacaacattc gcatcgtggc cgaaaacgcc atcggcacat cccaatcctc
     4621 tgaagcggtc actatcatca ctagtggatc ttccggaatc atcccagttg aggaggagaa
     4681 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     4741 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     4801 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     4861 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     4921 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     4981 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     5041 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     5101 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     5161 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     5221 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
     5281 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     5341 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     5401 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     5461 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     5521 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     5581 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     5641 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     5701 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     5761 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     5821 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     5881 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     5941 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     6001 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     6061 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     6121 gggtcatcat caccatcacc attaatctag agatcttatt aaagcagaac ttgtttattg
     6181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
     6301 cgactctagc tagactcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6901 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7141 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7201 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7261 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7321 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7381 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7441 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7501 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7561 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7621 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7681 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7741 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7801 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7861 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7921 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7981 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8041 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8101 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8161 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8221 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8281 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8341 gaaaacctct gacacatgca gctcccggag gaatgtgtgt cagttagggt gtggaaagtc
     8401 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8461 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8521 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8581 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8641 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8701 caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca cagcttgtct
     8761 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     8821 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     8881 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     8941 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9001 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9061 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9121 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9181 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9241 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9301 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9361 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     9421 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     9481 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     9541 aattgtcgag gtctcgacgg tatc
//
