LOCUS       sequence-232959-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase 
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.30 EC10-AP-His
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     557..577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     603..627
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          742..1567
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6139..6156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6182..6303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6219..6238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6273..6292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6341..6358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6512..7100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6592..6611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7271..8062,8063..8131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7894..7913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8132..8236)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8304..8322
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8360..8382)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8384..8404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8389..8718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8569..8704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8631..8650
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8855..8874
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8941..9396)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9078..9099)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9290..9309
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9522..9544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9536..9553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9537..9553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtctcgacgg tatcgattga cattgattat tgactagcta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      421 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      541 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      601 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      661 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      721 attccccgtg ccaagagtga cgtaagtacc gcctatagac tctataggca cacccctttg
      781 gctcttatgc atgctatact gtttttggct tggggcctat acacccccgc ttccatatgc
      841 tataggtgat ggtatagctt agcctatagg tgtgggttat tgaccattat tgaccactcc
      901 cctattggtg acgatacttt ccattactaa tccataacat ggctctttgc cacaactatc
      961 tctattggct atatgccaat actctgtcct tcagagactg acacggactc tgtattttta
     1021 caggatgggg tcccatttat tatttacaaa ttcacatata caacaacgcc gtcccccgtg
     1081 cccgcagttt ttattaaaca tagcgtggga tctccacgcg aatctcgggt acgtgttccg
     1141 gacatgggct cttctccggt agcggcggag cttccacatc cgagccctgg tcccatgcct
     1201 ccagcggctc atggtcgctc ggcagctcct tgctcctaac agtggaggcc agacttaggc
     1261 acagcacaat gcccaccacc accagtgtgc cgcacaaggc cgtggcggta gggtatgtgt
     1321 ctgaaaatga gctcggagat tgggctcgca ccgctgacgc agatggaaga cttaaggcag
     1381 cggcagaaga agatgcaggc agctgagttg ttgtattctg ataagagtca gaggtaactc
     1441 ccgttgcggt gctgttaacg gtggagggca gtgtagtctg agcagtactc gttgctgccg
     1501 cgcgcgccac cagacataat agctgacaga ctaacagact gttcctttcc atgggtcttt
     1561 tctgcagaag cttgaattct cgaggatccg cggccgctac tgccaacttc acacaacccg
     1621 ctcagcggaa acacggcata cgaaatgaat atgcccaacg aacgcctcaa atggctaatg
     1681 ctgttcgcag ctgttgccct cattgcttgt ggtagtcaga ccctagctgc caatccccca
     1741 gatgccgacc aaaaaggacc cgtcttcctc aaggaaccca ccaaccgcat tgacttctcc
     1801 aactccacgg gcgcagagat cgagtgcaag gccagcggca atcccatgcc cgagattatt
     1861 tggatcagga gcgacggtac cgccgtgggt gatgtgcccg gattgcgtca gatctcatcc
     1921 gacggcaagc tggtcttccc tcccttccgc gccgaggact accgccagga ggtccatgcc
     1981 caggtgtacg cctgcctggc ccgcaaccag ttcggatcca ttatctcccg ggacgtccat
     2041 gtgcgagccg ttgtcaatca gttctatgag gcggagatta tgaccgagta tgttatcagg
     2101 ggtaatgctg ccgttctgaa gtgttccatc ccgtcgttcg tggctgattt tgttcgagtt
     2161 gagtcctgga tcgatgatga gggcaatgtc ttgtcctttt cggacaatta cgatggaaag
     2221 tatctggtat tgccctctgg agagctccac atccgtgagg ttggacccga ggacggatac
     2281 aagagctacc agtgccgaac caaacatcgt ctgaccggag aaacccgatt aagtgccaca
     2341 aaaggacgat tggtcatcac agaacctgtg ggcagtgttg cgcctaagat cagcgtagaa
     2401 aatcgtctga aaaatgcaga ggctcgaatt ggttccacct tcaccctctt ttgccctggt
     2461 caagcctatc cagtaccctt ctttagatgg tacaagttca tcgaaggaac aacccgtaaa
     2521 caagctgtgg tgctaaatga ccgtgtgaag caggtctctg gtactctcat tattaaggac
     2581 gcagtagtcg aggactccgg aaaatacctg tgcgtggtga acaattccgt gggaggcgaa
     2641 agcgttgaga ctgtgcttac cgtcactgcc cccctatccg ctaagatcga tcctcccaca
     2701 cagacagttg acttcggacg ccccgccgta ttcacttgcc agtacactgg aaatcccatc
     2761 aagaccgtta gctggatgaa ggacggcaag gctatcggac acagcgaacc cgtgctgcgc
     2821 atcgaatccg tgaagaagga ggacaagggc atgtaccagt gcttcgtaag aaacgaccag
     2881 gaatcggcgg aggcgagtgc tgagctgaag ctcggaggcc gtttcgatcc ccccgtcatc
     2941 cgacaggcct tccaggaaga gaccatggaa cccggaccaa gtgtattcct caagtgcgtc
     3001 gccggcggaa accccacgcc cgaaatttcc tgggaactcg acggcaagaa aatcgccaac
     3061 aacgatcgct atcaggtcgg acagtatgtg acggtcaacg gagatgtggt ttcctatctg
     3121 aacataacct cggtccacgc caacgatggc ggtctctaca agtgcatagc caagagcaaa
     3181 gtgggcgtgg ccgaacactc ggccaagttg aatgtatatg gactgcccta catccggcaa
     3241 atggagaaga aggcaattgt tgctggagaa accctgattg tcacatgtcc tgtagctgga
     3301 taccccatcg attcgattgt ctgggagcga gataaccgtg ccctgcccat caaccgcaaa
     3361 cagaaggtct tccccaacgg aacgctgatt atcgagaatg tggaacggaa ctcagaccaa
     3421 gcgacctaca cttgcgttgc caagaatcag gaaggatact ctgctcgagg atctctggaa
     3481 gtgcaagtca tggttcttcc gcgcatcata cccttcgcct tcgaggaggg tcctgcccag
     3541 gtgggtcagt acctgacact gcactgctcc gttcccggcg gagatctgcc cctgaacatc
     3601 gactggacgc tggatggtca ggcaatctcg gaggatctgg gaataaccac gtcccgcgtg
     3661 ggtcgtcgtg gaagtgtgct aaccatcgag gccgtggagg cctcacatgc gggcaacttc
     3721 acctgccacg cccgcaattt ggcgggccat cagcagttca ccacgccgct gaacgtctac
     3781 gtacctccca gatggatcct cgagcccacc gataaggcct tcgcccaggg atccgatgcc
     3841 aaggttgaat gcaaggctga tggcttcccc aaaccccaag ttacatggaa gaaagcagtt
     3901 ggcgacaccc ccggagagta caaggatcta aagaagagcg acaacatccg cgtggaagag
     3961 ggcactctgc atgtcgacaa catccaaaag accaacgagg gctactatct gtgcgaggct
     4021 atcaatggaa taggctccgg cctctcggct gtgattatga tcagcgttca ggcccctcca
     4081 gagttcacgg agaagctgcg caaccagacc gcccgacgag gagaacccgc cgtactccag
     4141 tgcgaggcga agggcgagaa gcccattggc atcttgtgga acatgaacaa catgcgactg
     4201 gacccaaaga acgacaatcg gtacaccatt cgtgaggaga tcctttccac cggagttatg
     4261 tccagtctgt cgatcaagcg caccgagaga tccgactccg ccctattcac ctgtgtcgcc
     4321 actaatgcct ttggatccga tgacgccagc ataaatatga ttgtccagga agttccggag
     4381 atgccatatg ctttgaaggt actcgacaaa tccggacgtt ccgtgcagct gagctgggcg
     4441 caaccctacg atggcaactc ccctctggac aggtacatca ttgagtttaa gcgttctcgt
     4501 gcctcctgga gcgaaattga tcgcgtcatc gtgcccggac acaccacaga agcccaagtg
     4561 cagaagctca gccccgccac cacctacaac attcgcatcg tggccgaaaa cgccatcggc
     4621 acatcccaat cctctgaagc ggtcactatc atcactagtg gatcttccgg aatcatccca
     4681 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
     4741 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
     4801 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
     4861 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
     4921 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
     4981 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
     5041 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
     5101 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
     5161 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
     5221 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
     5281 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
     5341 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
     5401 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
     5461 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
     5521 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
     5581 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
     5641 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
     5701 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
     5761 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
     5821 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
     5881 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
     5941 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
     6001 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
     6061 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
     6121 gacgccgcgc acccgggtca tcatcaccat caccattaat ctagagatct tattaaagca
     6181 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6301 ttatcatgtc tggtcgactc tagctagact cttccgcttc ctcgctcact gactcgctgc
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8281 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     8341 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaggaatgt gtgtcagtta
     8401 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8461 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8521 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8641 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8701 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgt cccggagacg
     8761 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8821 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8881 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8941 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     9001 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     9061 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     9121 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     9181 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     9241 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     9301 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     9361 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     9421 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9481 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     9541 aacgacggcc agtgaattgt cgag
//
