LOCUS       sequence-232962-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human RNF169 and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-RNF169
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(230..235,236..253,254..260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     273..295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     292..308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     292..308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        333..581
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     333..353
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     504..523
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        603..678
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        713..1092
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1094..1371
                     /label=chicken beta-actin promoter
     intron          1372..2388
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2312..2334
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2401..2420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2401..2419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2421..2430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4571..4587)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4607..4831
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          5052..5990
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5947..5967
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6025..6034
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6031..6033,6034..6036,6037..6750)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6076..6097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    6781..6892
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7010..7027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7010..7026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7019..7041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7167..7622)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7304..7325)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7516..7535
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7649..7753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7754..7822,7823..8614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7972..7991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8785..9373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9274..9293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
       61 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      301 gctatgacca tgaggcgcgc ccccccttca ccgagggcct atttcccatg attccttcat
      361 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
      421 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
      481 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      541 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgactccagt ctttctagaa
      601 gagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      661 gtggcaccga gtcggtgctt tttttgatcc aagggtgggg cgcgccggat tcgacattga
      721 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      781 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      841 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      901 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      961 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     1021 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1081 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     1141 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     1201 gggggggggg gggggcgcgc gccrggsggg gsggggsggg gsgrggggsg gggsggggsg
     1261 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     1321 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
     1381 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     1441 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     1501 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     1561 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     1621 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     1681 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     1741 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     1801 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     1861 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     1921 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     1981 cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg cggctgtcga
     2041 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     2101 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     2161 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     2221 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     2281 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     2341 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagat ccttaattaa
     2401 taatacgact cactataggg gccaccatgg cggctgcagg tccgagtact cgggcctctt
     2461 ccgcggcggc agcagccgct ctgagtcggc ggggccggcg gggccgctgt gacgagacgg
     2521 cggcagctaa gactggggcc ccaggcccgg cttctggmcc ttcgctgttg gtgttgtcgc
     2581 cgccgttgct gcagccgccg ctgccgccgc ggccggagga atcgggctgc gccgggtgcc
     2641 tggagccccc cggagaagca gcggccctgc cgtgcggcca ctcgctttgc cgaggctgcg
     2701 cccaacgcgc cgccgacgcg gcgggcccgg gttgccctcg ctgccgcgcc cgcggcccag
     2761 gctgggcccg ccgtcgggcc cgcgacgacg gccaggccga ctcagaggtg ctgggcgagt
     2821 gcgcccgccg cagccaaccc gagcgctgcc gcccgcgccg ggacgggggc gcggctgccg
     2881 cggggcccag gccagagcag gagccgcgtg ccgcgcctgc ggagccagac tttatattca
     2941 gagcaccaat caaattaagc aagcctgggg aacttcgtga ggaatatgaa agcttgagaa
     3001 agctgagaga agaaaagtta caagaggaaa aaccctctga agatcaaatc cacaagctgt
     3061 taccagagga tacagaaaca gggaaaagga aaatggatga acagaaaaaa agagatgaac
     3121 cattagtact gaaaacaaat ctggaacgtt gtcctgcacg tctctcagat tcagagaatg
     3181 aagaaccttc tcgaggccag atgacacaga cacatcgctc ggcatttgtt tccaagaaca
     3241 actcctactc cttagctttc ctggcaggga agctaaactc caaggtggaa aggagtcaga
     3301 gctgtagtga cacagcccag gaaagagcga agagcagagt cagagcagtt ccaggcaaca
     3361 aagccaaggt cacaactatg actccagcct ccaaccccat cattggtgtc ctcttgtcaa
     3421 ctcaaaacaa ccgctgcgtc tcggcccctg acttaaccat cgaaaagcgt ctacccttca
     3481 gctccctttc atccttggct tccctgcata agccagagcg ttctgtcagc cctgagagca
     3541 atgacagcat ctccgaagaa ctaaaccatt tcaagcccat tgtctgctca ccatgtactc
     3601 ctcccaagag actccctgat ggccgtgtgc taagtcctct catcatcaaa tcaactccac
     3661 gcaacctaaa cagaagcctg cagaagcaga cttcttatga ggccagtcca cggatcctca
     3721 aaaagtggga acagatcttt caggagcggc agatcaaaaa gaccctttca aaagccactc
     3781 ttacctctct ggctcctgaa atgggggaag agttactagg ctctgaaggt atccattcta
     3841 gcaaggagaa gccacttgtg gctgtaaata caagattatc tggtgggcag gtcctctctg
     3901 agtatactgg acccacctct gctgatcttg atcatttccc ctctgttagc caaacaaaag
     3961 cagaacagga cagtgataat aaaagtagca ctgagatccc actggaaacc tgctgttcct
     4021 cagaactcaa agggggaggc agtgggactt ctttggagag ggagcagttt gaggggttag
     4081 ggtcaactcc agatgccaag ttagacaaaa cctgtataag cagagccatg aaaatcacca
     4141 cagttaattc agtgctaccc caaaacagtg ttttgggtgg agtcctcaaa acaaagcaac
     4201 aattgaagac attaaatcat tttgatctga ctaatggtgt tctagttgag agcctaagtg
     4261 aagagccact tccttctttg cgtcgaggcc ggaaaagaca ctgcaagacc aagcacttag
     4321 aacaaaatgg ctcccttaaa aaactgcgac aaaccagtgg ggaggtgggt ctggccccaa
     4381 cagacccagt cctgcgagag atggagcaga agcttcagca agaggaagaa gaccgacagt
     4441 tggctctgca gttgcagcgc atgttcgaca atgagaggcg gactgtgagc cggcgaaaag
     4501 gaagtgtgga tcagtatctc ctacggtcca gcaacatggc cggggccaag tagacgcgta
     4561 aatgattgca gatccactag ttctagagct cgctgatcag cctcgactgt gccttctagt
     4621 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4681 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4741 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4801 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4861 tcgagatcca ctagttctag cctcgaggct agagcggccc cggtgcctag agaaggtggc
     4921 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4981 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     5041 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     5101 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     5161 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     5221 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     5281 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     5341 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     5401 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     5461 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     5521 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     5581 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     5641 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     5701 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     5761 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     5821 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     5881 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     5941 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     6001 cgatcgcatc gacagtgttt aaacgccacc atggtgagca agggcgagga gctgttcacc
     6061 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     6121 aggggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
     6181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag
     6241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     6301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaccta caagacccgc
     6361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcgtcgac
     6421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac
     6481 atctatatca tggccgtcaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     6541 aacgtggagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     6601 gacggccccg tgctgctgcc cgacagccac tacctgagca cccagtccgt gctgagcaaa
     6661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tccgcaccgc cgccgggatc
     6721 actctcggca tggacgagct gtacaagtga gaattcctag agctcgctga tcagcctcga
     6781 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6841 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6901 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6961 gggaagagaa tagcaggcat gcgtttaaac attagtcgac agggccgcca ctggccgtcg
     7021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7081 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7141 agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc
     7201 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     7261 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     7321 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     7381 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     7441 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     7501 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     7561 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     7621 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7741 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7801 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7861 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7921 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7981 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8041 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8101 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8161 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8221 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8281 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8341 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8401 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8461 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8521 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8581 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8641 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8701 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8761 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8821 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8881 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8941 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9001 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9061 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9421 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggata
//
