LOCUS       sequence-232965-       12796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a fusion protein of human BRCA1 with the 
            human RNF169 Ubiquitin binding domain and BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-BRCA1-RNF169UBD
FEATURES             Location/Qualifiers
     source          1..12796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(144..149,150..167,168..174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    182..198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     187..209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     206..222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     206..222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        242..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          901..1917
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1841..1863
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        1930..1948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1953..1962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7600..7618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7620..7629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7629..7652
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7814..7830)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7850..8074
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          8295..9233
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9190..9210
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      9268..9277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9274..9276,9277..9279,9280..9993)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9319..9340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    10024..10135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10253..10270)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10253..10269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10262..10284)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10410..10865)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10547..10568)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10759..10778
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10892..10996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10997..11065,11066..11857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11215..11234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12028..12616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12517..12536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12770..12787
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
       61 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      181 attgtgagcg gataacaatt tcacacagga aacagctatg accatgaggc gcgccggatt
      241 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      661 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      721 atgggggcgg gggggggggg ggggcgcgcg ccaggsgggg sggggsgggg sgrggggggg
      781 ggsggggsgr ggsggrgrgg kgsggcggca gccaatcaga gcggcgcgct ccgaaagttt
      841 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      901 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      961 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1021 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
     1081 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
     1141 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
     1201 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1261 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1321 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1381 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1441 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1501 gggccgccyc gggccggggr gggctcgggg grggggcgcg gcggcccccg gagcgccggc
     1561 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1621 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1681 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1741 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1801 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1861 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagatc
     1921 cttaattaat aatacgactc actatagggg ccgccaccat ggatttatct gctcttcgcg
     1981 ttgaagaagt acaaaatgtc attaatgcta tgcagaaaat cttagagtgt cccatctgtc
     2041 tggagttgat caaggaacct gtctccacaa agtgtgacca catattttgc aaattttgca
     2101 tgctgaaact tctcaaccag aagaaagggc cttcacagtg tcctttatgt aagaatgata
     2161 taaccaaaag gagcctacaa gaaagtacga gatttagtca acttgttgaa gagctattga
     2221 aaatcatttg tgcttttcag cttgacacag gtttggagta tgcaaacagc tataattttg
     2281 caaaaaagga aaataactct cctgaacatc taaaagatga agtttctatc atccaaagta
     2341 tgggctacag aaaccgtgcc aaaagacttc tacagagtga acccgaaaat ccttccttgc
     2401 aggaaaccag tctcagtgtc caactctcta accttggaac tgtgagaact ctgaggacaa
     2461 agcagcggat acaacctcaa aagacgtctg tctacattga attgggatct gattcttctg
     2521 aagataccgt taataaggca acttattgca gtgtgggaga tcaagaattg ttacaaatca
     2581 cccctcaagg aaccagggat gaaatcagtt tggattctgc aaaaaaggct gcttgtgaat
     2641 tttctgagac ggatgtaaca aatactgaac atcatcaacc cagtaataat gatttgaaca
     2701 ccactgagaa gcgtgcagct gagaggcatc cagaaaagta tcagggtagt tctgtttcaa
     2761 acttgcatgt ggagccatgt ggcacaaata ctcatgccag ctcattacag catgagaaca
     2821 gcagtttatt actcactaaa gacagaatga atgtagaaaa ggctgaattc tgtaataaaa
     2881 gcaaacagcc tggcttagca aggagccaac ataacagatg ggctggaagt aaggaaacat
     2941 gtaatgatag gcggactccc agcacagaaa aaaaggtaga tctgaatgct gatcccctgt
     3001 gtgagagaaa agaatggaat aagcagaaac tgccatgctc agagaatcct agagatactg
     3061 aagatgttcc ttggataaca ctaaatagca gcattcagaa agttaatgag tggttttcca
     3121 gaagtgatga actgttaggt tctgatgact cacatgatgg ggagtctgaa tcaaatgcca
     3181 aagtagctga tgtattggac gttctaaatg aggtagatga atattctggt tcttcagaga
     3241 aaatagactt actggccagt gatcctcatg aggctttaat atgtaaaagt gaaagagttc
     3301 actccaaatc agtagagagt aatattgaag acaaaatatt tgggaaaacc tatcggaaga
     3361 aggcaagcct ccccaactta agccatgtaa ctgaaaatct aattatagga gcatttgtta
     3421 ctgagccaca gataatacaa gagcgtcccc tcacaaataa attaaagcgt aaaaggagac
     3481 ctacatcagg ccttcatcct gaggatttta tcaagaaagc agatttggca gttcaaaaga
     3541 ctcctgaaat gataaatcag ggaactaacc aaacggagca gaatggtcaa gtgatgaata
     3601 ttactaatag tggtcatgag aataaaacaa aaggtgattc tattcagaat gagaaaaatc
     3661 ctaacccaat agaatcactc gaaaaagaat ctgctttcaa aacgaaagct gaacctataa
     3721 gcagcagtat aagcaatatg gaactcgaat taaatatcca caattcaaaa gcacctaaaa
     3781 agaataggct gaggaggaag tcttctacca ggcatattca tgcgcttgaa ctagtagtca
     3841 gtagaaatct aagcccacct aattgtactg aattgcaaat tgatagttgt tctagcagtg
     3901 aagagataaa gaaaaaaaag tacaaccaaa tgccagtcag gcacagcaga aacctacaac
     3961 tcatggaagg taaagaacct gcaactggag ccaagaagag taacaagcca aatgaacaga
     4021 caagtaaaag acatgacagt gatactttcc cagagctgaa gttaacaaat gcacctggtt
     4081 cttttactaa gtgttcaaat accagtgaac ttaaagaatt tgtcaatcct agccttccaa
     4141 gagaagaaaa agaagagaaa ctagaaacag ttaaagtgtc taataatgct gaagacccca
     4201 aagatctcat gttaagtgga gaaagggttt tgcaaactga aagatctgta gagagtagca
     4261 gtatttcact ggtacctggt actgattatg gcactcagga aagtatctcg ttactggaag
     4321 ttagcactct agggaaggca aaaacagaac caaataaatg tgtgagtcag tgtgcagcat
     4381 ttgaaaaccc caagggacta attcatggtt gttccaaaga taatagaaat gacacagaag
     4441 gctttaagta tccattggga catgaagtta accacagtcg ggaaacaagc atagaaatgg
     4501 aagaaagtga acttgatgct cagtatttgc agaatacatt caaggtttca aagcgccagt
     4561 catttgctct gttttcaaat ccaggaaatg cagaagagga atgtgcaaca ttctctgccc
     4621 actctgggtc cttaaagaaa caaagtccaa aagtcacttt tgaatgtgaa caaaaggaag
     4681 aaaatcaagg aaagaatgag tctaatatca agcctgtaca gacagttaat atcactgcag
     4741 gctttcctgt ggttggtcag aaagataagc cagttgataa tgccaaatgt agtatcaaag
     4801 gaggctctag gttttgtcta tcatctcagt tcagaggcaa cgaaactgga ctcattactc
     4861 caaataaaca tggactttta caaaacccat atcgtatacc accacttttt cccatcaagt
     4921 catttgttaa aactaaatgt aagaaaaatc tgctagagga aaactttgag gaacattcaa
     4981 tgtcacctga aagagaaatg ggaaatgaga acattccaag tacagtgagc acaattagcc
     5041 gtaataacat tagagaaaat gtttttaaag gagccagctc aagcaatatt aatgaagtag
     5101 gttccagtac taatgaagtg ggctccagta ttaatgaaat aggttccagt gatgaaaaca
     5161 ttcaagcaga actaggtaga aacagagggc caaaattgaa tgctatgctt agattagggg
     5221 ttttgcaacc tgaggtctat aaacaaagtc ttcctggaag taattgtaag catcctgaaa
     5281 taaaaaagca agaatatgaa gaagtagttc agactgttaa tacagatttc tctccatatc
     5341 tgatttcaga taacttagaa cagcctatgg gaagtagtca tacatctcag gtttgttctg
     5401 agacacctga tgacctgtta gatgatggtg aaataaagga agatactagt tttgctgaaa
     5461 atgacattaa ggaaagttct gctgttttta gcaaaagcgt ccagagagga gagcttagca
     5521 ggagtcctag ccctttcacc catacacatt tggctcaggg ttaccgaaga ggggccaaga
     5581 aattagagtc ctcagaagag aacttatcta gtgaggatga agagcttccc tgcttccaac
     5641 acttgttatt tggtaaagta aacaatatac cttctcagtc tactaggcat agcaccgttg
     5701 ctaccgagtg tctgtctaag aacacagagg agaatttatt atcattgaag aatagcttaa
     5761 atgactgcag taaccaggta atattggcaa aggcatctca ggaacatcac cttagtgagg
     5821 aaacaaaatg ttctgctagc ttgttttctt cacagtgcag tgaattggaa gacttgactg
     5881 caaatacaaa cacccaggat cctttcttga ttggttcttc caaacaaatg aggcatcagt
     5941 ctgaaagcca gggagttggt ctgagtgaca aggaattggt ttcagatgat gaagaaagag
     6001 gaacgggctt ggaagaaaat aatcaagaag agcaaagcat ggattcaaac ttaggtgaag
     6061 cagcatctgg gtgtgagagt gaaacaagcg tctctgaaga ctgctcaggg ctatcctctc
     6121 agagtgacat tttaaccact cagcagaggg ataccatgca acataacctg ataaagctcc
     6181 agcaggaaat ggctgaacta gaagctgtgt tagaacagca tgggagccag ccttctaaca
     6241 gctaccctag tataatcagt gactcttctg cccttgagga cctgcgaaat ccagaacaaa
     6301 gcacatcaga aaaagcagta ttaacttcac agaaaagtag tgaataccct ataagccaga
     6361 atccagaagg cctttctgct gacaagtttg aggtgtctgc agatagttct accagtaaaa
     6421 ataaagaacc aggagtggaa aggtcatccc cttctaaatg cccatcatta gatgataggt
     6481 ggtacatgca cagttgctct gggagtcttc agaatagaaa ctacccatct caagaggagc
     6541 tcattaaggt tgttgatgtg gaggagcaac agctggaaga gtctgggcca cacgatttga
     6601 cggaaacatc ttacttgcca aggcaagatc tagagggaac cccttacctg gaatctggaa
     6661 tcagcctctt ctctgatgac cctgaatctg atccttctga agacagagcc ccagagtcag
     6721 ctcgtgttgg caacatacca tcttcaacct ctgcattgaa agttccccaa ttgaaagttg
     6781 cagaatctgc ccagggtcca gctgctgctc atactactga tactgctggg tataatgcaa
     6841 tggaagaaag tgtgagcagg gagaagccag aattgacagc ttcaacagaa agggtcaaca
     6901 aaagaatgtc catggtggtg agtggcctga ccccagaaga atttatgctc gtgtacaagt
     6961 ttgccagaaa acaccacatc actttaacta atctaattac tgaagagact actcatgttg
     7021 ttatgaaaac agatgctgag tttgtgtgtg aacggacact gaaatatttt ctaggaattg
     7081 cgggaggaaa atgggtagtt agctatttct gggtgaccca gtctattaaa gaaagaaaaa
     7141 tgctgaatga gcatgatttt gaagtcagag gagatgtggt caatggaaga aaccaccaag
     7201 gtccaaagcg agcaagagaa tcccaggaca gaaagatctt cagggggcta gaaatctgtt
     7261 gctatgggcc cttcaccaac atgcccacag atcaactgga atggatggta cagctgtgtg
     7321 gtgcttctgt ggtgaaggag ctttcatcat tcacccttgg cacaggtgtc cacccaattg
     7381 tggttgtgca gccagatgcc tggacagagg acaatggctt ccatgcaatt gggcagatgt
     7441 gtgaggcacc tgtggtgacc cgagagtggg tgttggacag tgtagcactc taccagtgcc
     7501 aggagctgga cacctacctg ataccccaga tcccccacag ccactacgga ggaggtggaa
     7561 gcggaggagg aggaagcgga ggaggaggta gccgattaat aatacgactc actatagggg
     7621 ccaccatgga ctacaaggat gacgacgata aaaagcttca gcaagaggaa gaagaccgac
     7681 agttggctct gcagttgcag cgcatgttcg acaatgagag gcggactgtg agccggcgaa
     7741 aaggaagtgt ggatcagtat ctcctacggt ccagcaacat ggccggggcc aagtagacgc
     7801 gtaaatgatt gcagatccac tagttctaga gctcgctgat cagcctcgac tgtgccttct
     7861 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7921 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7981 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8041 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8101 ggctcgagat ccactagttc tagcctcgag gctagagcgg ccccggtgcc tagagaaggt
     8161 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     8221 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     8281 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     8341 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     8401 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     8461 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     8521 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     8581 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     8641 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     8701 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     8761 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     8821 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     8881 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     8941 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     9001 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     9061 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     9121 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     9181 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     9241 gagcgatcgc atcgacagtg tttaaacgcc accatggtga gcaagggcga ggagctgttc
     9301 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     9361 gtgaggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
     9421 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ccacggcgtg
     9481 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     9541 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcac ctacaagacc
     9601 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcgtc
     9661 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
     9721 aacatctata tcatggccgt caagcagaag aacggcatca aggtgaactt caagatccgc
     9781 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     9841 ggcgacggcc ccgtgctgct gcccgacagc cactacctga gcacccagtc cgtgctgagc
     9901 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttccgcac cgccgccggg
     9961 atcactctcg gcatggacga gctgtacaag tgagaattcc tagagctcgc tgatcagcct
    10021 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    10081 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    10141 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    10201 attgggaaga gaatagcagg catgcgttta aacattagtc gacagggccg ccactggccg
    10261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    10321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    10381 aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt
    10441 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
    10501 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
    10561 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
    10621 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
    10681 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
    10741 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
    10801 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
    10861 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    10921 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    10981 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    11041 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    11101 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    11161 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    11221 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    11281 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    11341 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    11401 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    11461 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    11521 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    11581 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    11641 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    11701 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    11761 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    11821 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    11881 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    11941 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    12001 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    12061 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    12121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    12181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    12241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    12301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    12361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    12421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    12481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    12541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    12661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    12721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    12781 gagcgaggaa gcggaa
//
