LOCUS       sequence-232966-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.22 protein, C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.22 EC10-Fc-His
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        325..528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     479..499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     525..549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          664..1489
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5269..5286
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5312..5433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5349..5368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5403..5422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5471..5488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5642..6230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5722..5741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6401..7192,7193..7261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7024..7043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7262..7366)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7434..7452
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7490..7512)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7514..7534)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7519..7848
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7699..7834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7761..7780
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7985..8004
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8071..8526)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8208..8229)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8420..8439
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8652..8674
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8666..8683
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8667..8683
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(8713..8772,1..324)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
       61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      181 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
      241 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
      301 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
      361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
      481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      601 cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt
      661 gacgtaagta ccgcctatag actctatagg cacacccctt tggctcttat gcatgctata
      721 ctgtttttgg cttggggcct atacaccccc gcttccatat gctataggtg atggtatagc
      781 ttagcctata ggtgtgggtt attgaccatt attgaccact cccctattgg tgacgatact
      841 ttccattact aatccataac atggctcttt gccacaacta tctctattgg ctatatgcca
      901 atactctgtc cttcagagac tgacacggac tctgtatttt tacaggatgg ggtcccattt
      961 attatttaca aattcacata tacaacaacg ccgtcccccg tgcccgcagt ttttattaaa
     1021 catagcgtgg gatctccacg cgaatctcgg gtacgtgttc cggacatggg ctcttctccg
     1081 gtagcggcgg agcttccaca tccgagccct ggtcccatgc ctccagcggc tcatggtcgc
     1141 tcggcagctc cttgctccta acagtggagg ccagacttag gcacagcaca atgcccacca
     1201 ccaccagtgt gccgcacaag gccgtggcgg tagggtatgt gtctgaaaat gagctcggag
     1261 attgggctcg caccgctgac gcagatggaa gacttaaggc agcggcagaa gaagatgcag
     1321 gcagctgagt tgttgtattc tgataagagt cagaggtaac tcccgttgcg gtgctgttaa
     1381 cggtggaggg cagtgtagtc tgagcagtac tcgttgctgc cgcgcgcgcc accagacata
     1441 atagctgaca gactaacaga ctgttccttt ccatgggtct tttctgcaga agcttgaatt
     1501 ctcgaggatc cgcggccgct actgccaact tcacacaacc cgctcagcgg aaacacggca
     1561 tacgaaatga atatgcccaa cgaacgcctc aaatggctaa tgctgttcgc agctgttgcc
     1621 ctcattgctt gtggtagtca gaccctagct gccaatcccc cagatgccga ccaaaaagga
     1681 cccgtcttcc tcaaggaacc caccaaccgc attgacttct ccaactccac gggcgcagag
     1741 atcgagtgca aggccagcgg caatcccatg cccgagatta tttggatcag gagcgacggt
     1801 accgccgtgg gtgatgtgcc cggattgcgt cagatctcat ccgacggcaa gctggtcttc
     1861 cctcccttcc gcgccgagga ctaccgccag gaggtccatg cccaggtgta cgcctgcctg
     1921 gcccgcaacc agttcggatc cattatctcc cgggacgtcc atgtgcgagc cgttgtcaat
     1981 cagttctatg aggcggagat tatgaccgag tatgttatca ggggtaatgc tgccgttctg
     2041 aagtgttcca tcccgtcgtt cgtggctgat tttgttcgag ttgagtcctg gatcgatgat
     2101 gagggcaatg tcttgtcctt ttcggacaat tacgatggaa agtatctggt attgccctct
     2161 ggagagctgc acatccgtga ggttggaccc gaggacggat acaagagcta ccagtgccga
     2221 accaaacatc gtctgaccgg agaaacccga ttaagtgcca caaaaggacg attggtcatc
     2281 acagaacctg tgggcagtgt tgcgcctaag atcagcgtag aaaatcgtct gaaaaatgca
     2341 gaggctcgaa ttggttccac cttcaccctc ttttgccctg gtcaagccta tccagtaccc
     2401 ttctttagat ggtacaagtt catcgaagga acaacccgta aacaagctgt ggtgctaaat
     2461 gaccgtgtga agcaggtctc tggtactctc attattaagg acgcagtagt cgaggactcc
     2521 ggaaaatacc tgtgcgtggt gaacaattcc gtgggaggcg aaagcgttga gactgtgctt
     2581 accgtcactg cccccctatc cgctaagatc gatcctccca cacagacagt tgacttcgga
     2641 cgccccgccg tattcacttg ccagtacact ggaaatccca tcaagaccgt tagctggatg
     2701 aaggacggca aggctatcgg acacagcgaa cccgtgctgc gcatcgaatc cgtgaagaag
     2761 gaggacaagg gcatgtacca gtgcttcgta agaaacgacc aggaatcggc ggaggcgagt
     2821 gctgagctga agctcggagg ccgtttcgat ccccccgtca tccgacaggc cttccaggaa
     2881 gagaccatgg aacccggacc aagtgtattc ctcaagtgcg tcgccggcgg aaaccccacg
     2941 cccgaaattt cctgggaact cgacggcaag aaaatcgcca acaacgatcg ctatcaggtc
     3001 ggacagtatg tgacggtcaa cggagatgtg gtttcctatc tgaacataac ctcggtccac
     3061 gccaacgatg gcggtctcta caagtgcata gccaagagca aagtgggcgt ggccgaacac
     3121 tcggccaagt tgaatgtata tggactgccc tacatccggc aaatggagaa gaaggcaatt
     3181 gttgctggag aaaccctgat tgtcacatgt cctgtagctg gataccccat cgattcgatt
     3241 gtctgggagc gagataaccg tgccctgccc atcaaccgca aacagaaggt cttccccaac
     3301 ggaacgctga ttatcgagaa tgtggaacgg aactcagacc aagcgaccta cacttgcgtt
     3361 gccaagaatc aggaaggata ctctgctcga ggatctctgg aagtgcaagt catggttccc
     3421 ccgaaaatca cacctttcac ctttggcgag gatcccacca atgtggagga ctcagtctct
     3481 gtgacttgtt tgatatccag tggagactta cccatcgata tcgagtggct atttaatgac
     3541 ttcggaatca gctcgtattc aggaatgact gtcctcagag gtggaaagag aaccagcatg
     3601 ctgaccatag ataatgtcca tgctcgtcat gccggaaagt atagttgtcg cgcaaagaac
     3661 cacgcagcag ccgtcaacta cactactgag ttgattgtga atgtacctcc cagatggatc
     3721 ctcgagccca ccgataaggc cttcgcccag ggatccgatg ccaaggttga atgcaaggct
     3781 gatggcttcc ccaaacccca agttacatgg aagaaagcag ttggcgacac ccccggagag
     3841 tacaaggatc taaagaagag cgacaacatc cgcgtggaag agggcactct gcatgtcgac
     3901 aacatccaaa agaccaacga gggctactat ctgtgcgagg ctatcaatgg aataggctcc
     3961 ggcctctcgg ctgtgattat gatcagcgtt caggcccctc cagagttcac ggagaagctg
     4021 cgcaaccaga ccgcccgacg aggagaaccc gccgtactcc agtgcgaggc gaagggcgag
     4081 aagcccattg gcatcttgtg gaacatgaac aacatgcgac tggacccaaa gaacgacaat
     4141 cggtacacca ttcgtgagga gatcctttcc accggagtta tgtccagtct gtcgatcaag
     4201 cgcaccgaga gatccgactc cgccctattc acctgtgtcg ccactaatgc ctttggatcc
     4261 gatgacgcca gcataaatat gattgtccag gaagttccgg agatgccata tgctttgaag
     4321 gtactcgaca aatccggacg ttccgtgcag ctgagctggg cgcaacccta cgatggcaac
     4381 tcccctctgg acaggtacat cattgagttt aagcgttctc gtgcctcctg gagcgaaatt
     4441 gatcgcgtca tcgtgcccgg acacaccaca gaagcccaag tgcagaagct cagccccgcc
     4501 accacctaca acattcgcat cgtggccgaa aacgccatcg gcacatccca atcctctgaa
     4561 gcggtcacta tcatcactag tgacaaaact cacacatgcc caccgtgccc agcacctgaa
     4621 ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc
     4681 tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc
     4741 aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag
     4801 gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg
     4861 ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag
     4921 aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtatac cctgccccca
     4981 tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat
     5041 cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc
     5101 acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac
     5161 aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac
     5221 aaccactaca cgcagaagag cctctccctg tctccgggta aaggcggcca tcatcaccat
     5281 caccattaat ctagagatct tattaaagca gaacttgttt attgcagctt ataatggtta
     5341 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5401 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggtcgactc tagctagact
     5461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     7441 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     7501 tgcagctccc ggaggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7801 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7861 gcttgtatat ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7921 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7981 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8041 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     8101 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     8161 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     8221 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     8281 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     8341 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     8401 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     8461 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     8521 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt cgaggtctcg
     8701 acggtatcga ttgacattga ttattgacta gctagttatt aatagtaatc aattacgggg
     8761 tcattagttc at
//
