LOCUS       sequence-232970-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.23 protein, C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.23 EC10-Fc-His
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     571..595
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          710..1535
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5315..5332
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5358..5479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5395..5414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5449..5468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5517..5534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5688..6276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5768..5787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6447..7238,7239..7307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7070..7089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7308..7412)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7480..7498
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7536..7558)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7560..7580)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7565..7894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7745..7880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7807..7826
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8031..8050
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8117..8572)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8254..8275)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8466..8485
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8698..8720
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8712..8729
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8713..8729
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(8759..8772,1..370)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagctagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      601 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
      661 cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc
      721 ctatagactc tataggcaca cccctttggc tcttatgcat gctatactgt ttttggcttg
      781 gggcctatac acccccgctt ccatatgcta taggtgatgg tatagcttag cctataggtg
      841 tgggttattg accattattg accactcccc tattggtgac gatactttcc attactaatc
      901 cataacatgg ctctttgcca caactatctc tattggctat atgccaatac tctgtccttc
      961 agagactgac acggactctg tatttttaca ggatggggtc ccatttatta tttacaaatt
     1021 cacatataca acaacgccgt cccccgtgcc cgcagttttt attaaacata gcgtgggatc
     1081 tccacgcgaa tctcgggtac gtgttccgga catgggctct tctccggtag cggcggagct
     1141 tccacatccg agccctggtc ccatgcctcc agcggctcat ggtcgctcgg cagctccttg
     1201 ctcctaacag tggaggccag acttaggcac agcacaatgc ccaccaccac cagtgtgccg
     1261 cacaaggccg tggcggtagg gtatgtgtct gaaaatgagc tcggagattg ggctcgcacc
     1321 gctgacgcag atggaagact taaggcagcg gcagaagaag atgcaggcag ctgagttgtt
     1381 gtattctgat aagagtcaga ggtaactccc gttgcggtgc tgttaacggt ggagggcagt
     1441 gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag ctgacagact
     1501 aacagactgt tcctttccat gggtcttttc tgcagaagct tgaattctcg aggatccgcg
     1561 gccgctactg ccaacttcac acaacccgct cagcggaaac acggcatacg aaatgaatat
     1621 gcccaacgaa cgcctcaaat ggctaatgct gttcgcagct gttgccctca ttgcttgtgg
     1681 tagtcagacc ctagctgcca atcccccaga tgccgaccaa aaaggacccg tcttcctcaa
     1741 ggaacccacc aaccgcattg acttctccaa ctccacgggc gcagagatcg agtgcaaggc
     1801 cagcggcaat cccatgcccg agattatttg gatcaggagc gacggtaccg ccgtgggtga
     1861 tgtgcccgga ttgcgtcaga tctcatccga cggcaagctg gtcttccctc ccttccgcgc
     1921 cgaggactac cgccaggagg tccatgccca ggtgtacgcc tgcctggccc gcaaccagtt
     1981 cggatccatt atctcccggg acgtccatgt gcgagccgtt gtcaatcagt tctatgaggc
     2041 ggagattatg accgagtatg ttatcagggg taatgctgcc gttctgaagt gttccatccc
     2101 gtcgttcgtg gctgattttg ttcgagttga gtcctggatc gatgatgagg gcaatgtctt
     2161 gtccttttcg gacaattacg atggaaagta tctggtattg ccctctggag agctgcacat
     2221 ccgtgaggtt ggacccgagg acggatacaa gagctaccag tgccgaacca aacatcgtct
     2281 gaccggagaa acccgattaa gtgccacaaa aggacgattg gtcatcacag aacctgtggg
     2341 cagtgttgcg cctaagatca gcgtagaaaa tcgtctgaaa aatgcagagg ctcgaattgg
     2401 ttccaccttc accctctttt gccctggtca agcctatcca gtacccttct ttagatggta
     2461 caagttcatc gaaggaacaa cccgtaaaca agctgtggtg ctaaatgacc gtgtgaagca
     2521 ggtctctggt actctcatta ttaaggacgc agtagtcgag gactccggaa aatacctgtg
     2581 cgtggtgaac aattccgtgg gaggcgaaag cgttgagact gtgcttaccg tcactgcccc
     2641 cctatccgct aagatcgatc ctcccacaca gacagttgac ttcggacgcc ccgccgtatt
     2701 cacttgccag tacactggaa atcccatcaa gaccgttagc tggatgaagg acggcaaggc
     2761 tatcggacac agcgaacccg tgctgcgcat cgaatccgtg aagaaggagg acaagggcat
     2821 gtaccagtgc ttcgtaagaa acgaccagga atcggcggag gcgagtgctg agctgaagct
     2881 cggaggccgt ttcgatcccc ccgtcatccg acaggccttc caggaagaga ccatggaacc
     2941 cggaccaagt gtattcctca agtgcgtcgc cggcggaaac cccacgcccg aaatttcctg
     3001 ggaactcgac ggcaagaaaa tcgccaacaa cgatcgctat caggtcggac agtatgtgac
     3061 ggtcaacgga gatgtggttt cctatctgaa cataacctcg gtccacgcca acgatggcgg
     3121 tctctacaag tgcatagcca agagcaaagt gggcgtggcc gaacactcgg ccaagttgaa
     3181 tgtatatgga ctgccctaca tccggcaaat ggagaagaag gcaattgttg ctggagaaac
     3241 cctgattgtc acatgtcctg tagctggata ccccatcgat tcgattgtct gggagcgaga
     3301 taaccgtgcc ctgcccatca accgcaaaca gaaggtcttc cccaacggaa cgctgattat
     3361 cgagaatgtg gaacggaact cagaccaagc gacctacact tgcgttgcca agaatcagga
     3421 aggatactct gctcgaggat ctctggaagt gcaagtcatg gttcctccta aaatcactcc
     3481 cttcaccttc ggcgatgaac ctaccaatgt ggaggattcg gtttcggtga cctgcttgat
     3541 ctccagcgga gacctaccca tcgacataga gtggctattt aatgactacg ggattagctc
     3601 cttctccggg atgaccgttt atagaggagg aaagagaacg agcatgctga ccatagacaa
     3661 tgtccatgct cgccacgcgg gcaaatatag ttgtcgagcg agaaatcatg cgtctgcggt
     3721 taatttcaca accgagttga ttgtgaatgt acctcccaga tggatcctcg agcccaccga
     3781 taaggccttc gcccagggat ccgatgccaa ggttgaatgc aaggctgatg gcttccccaa
     3841 accccaagtt acatggaaga aagcagttgg cgacaccccc ggagagtaca aggatctaaa
     3901 gaagagcgac aacatccgcg tggaagaggg cactctgcat gtcgacaaca tccaaaagac
     3961 caacgagggc tactatctgt gcgaggctat caatggaata ggctccggcc tctcggctgt
     4021 gattatgatc agcgttcagg cccctccaga gttcacggag aagctgcgca accagaccgc
     4081 ccgacgagga gaacccgccg tactccagtg cgaggcgaag ggcgagaagc ccattggcat
     4141 cttgtggaac atgaacaaca tgcgactgga cccaaagaac gacaatcggt acaccattcg
     4201 tgaggagatc ctttccaccg gagttatgtc cagtctgtcg atcaagcgca ccgagagatc
     4261 cgactccgcc ctattcacct gtgtcgccac taatgccttt ggatccgatg acgccagcat
     4321 aaatatgatt gtccaggaag ttccggagat gccatatgct ttgaaggtac tcgacaaatc
     4381 cggacgttcc gtgcagctga gctgggcgca accctacgat ggcaactccc ctctggacag
     4441 gtacatcatt gagtttaagc gttctcgtgc ctcctggagc gaaattgatc gcgtcatcgt
     4501 gcccggacac accacagaag cccaagtgca gaagctcagc cccgccacca cctacaacat
     4561 tcgcatcgtg gccgaaaacg ccatcggcac atcccaatcc tctgaagcgg tcactatcat
     4621 cactagtgac aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc
     4681 gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga
     4741 ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta
     4801 cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag
     4861 cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga
     4921 gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa
     4981 agccaaaggg cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct
     5041 gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc
     5101 cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct
     5161 ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca
     5221 gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca
     5281 gaagagcctc tccctgtctc cgggtaaagg cggccatcat caccatcacc attaatctag
     5341 agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5401 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5461 aactcatcaa tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg
     5521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5581 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5641 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5761 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5821 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5881 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5941 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6001 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6061 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6121 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6181 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6241 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6301 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6361 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6421 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6481 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6541 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6601 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6661 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6721 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6781 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6841 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6901 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6961 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7021 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7081 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7141 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7201 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7261 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7321 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7381 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7441 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     7501 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     7561 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7801 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7861 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7921 tttcgtcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7981 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     8041 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     8101 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8161 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8221 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8281 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8341 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8401 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8461 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8521 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
     8581 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     8641 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     8701 agtcacgacg ttgtaaaacg acggccagtg aattgtcgag gtctcgacgg tatcgattga
     8761 cattgattat tg
//
