LOCUS       sequence-232974-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.25 protein, C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.25 EC10-Fc-His
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        367..570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     521..541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     567..591
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          706..1531
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5311..5328
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5354..5475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5391..5410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5445..5464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5513..5530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5684..6272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5764..5783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6443..7234,7235..7303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7066..7085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7304..7408)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7476..7494
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7532..7554)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7556..7576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7561..7890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7741..7876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7803..7822
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8027..8046
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8113..8568)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8250..8271)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8462..8481
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8694..8716
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8708..8725
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8709..8725
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(8755..8772,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
      601 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccgcggc
      661 cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat
      721 agactctata ggcacacccc tttggctctt atgcatgcta tactgttttt ggcttggggc
      781 ctatacaccc ccgcttccat atgctatagg tgatggtata gcttagccta taggtgtggg
      841 ttattgacca ttattgacca ctcccctatt ggtgacgata ctttccatta ctaatccata
      901 acatggctct ttgccacaac tatctctatt ggctatatgc caatactctg tccttcagag
      961 actgacacgg actctgtatt tttacaggat ggggtcccat ttattattta caaattcaca
     1021 tatacaacaa cgccgtcccc cgtgcccgca gtttttatta aacatagcgt gggatctcca
     1081 cgcgaatctc gggtacgtgt tccggacatg ggctcttctc cggtagcggc ggagcttcca
     1141 catccgagcc ctggtcccat gcctccagcg gctcatggtc gctcggcagc tccttgctcc
     1201 taacagtgga ggccagactt aggcacagca caatgcccac caccaccagt gtgccgcaca
     1261 aggccgtggc ggtagggtat gtgtctgaaa atgagctcgg agattgggct cgcaccgctg
     1321 acgcagatgg aagacttaag gcagcggcag aagaagatgc aggcagctga gttgttgtat
     1381 tctgataaga gtcagaggta actcccgttg cggtgctgtt aacggtggag ggcagtgtag
     1441 tctgagcagt actcgttgct gccgcgcgcg ccaccagaca taatagctga cagactaaca
     1501 gactgttcct ttccatgggt cttttctgca gaagcttgaa ttctcgagga tccgcggccg
     1561 ctactgccaa cttcacacaa cccgctcagc ggaaacacgg catacgaaat gaatatgccc
     1621 aacgaacgcc tcaaatggct aatgctgttc gcagctgttg ccctcattgc ttgtggtagt
     1681 cagaccctag ctgccaatcc cccagatgcc gaccaaaaag gacccgtctt cctcaaggaa
     1741 cccaccaacc gcattgactt ctccaactcc acgggcgcag agatcgagtg caaggccagc
     1801 ggcaatccca tgcccgagat tatttggatc aggagcgacg gtaccgccgt gggtgatgtg
     1861 cccggattgc gtcagatctc atccgacggc aagctggtct tccctccctt ccgcgccgag
     1921 gactaccgcc aggaggtcca tgcccaggtg tacgcctgcc tggcccgcaa ccagttcgga
     1981 tccattatct cccgggacgt ccatgtgcga gccgttgtca atcagttcta tgaggcggag
     2041 attatgaccg agtatgttat caggggtaat gctgccgttc tgaagtgttc catcccgtcg
     2101 ttcgtggctg attttgttcg agttgagtcc tggatcgatg atgagggcaa tgtcttgtcc
     2161 ttttcggaca attacgatgg aaagtatctg gtattgccct ctggagagct gcacatccgt
     2221 gaggttggac ccgaggacgg atacaagagc taccagtgcc gaaccaaaca tcgtctgacc
     2281 ggagaaaccc gattaagtgc cacaaaagga cgattggtca tcacagaacc tgtgggcagt
     2341 gttgcgccta agatcagcgt agaaaatcgt ctgaaaaatg cagaggctcg aattggttcc
     2401 accttcaccc tcttttgccc tggtcaagcc tatccagtac ccttctttag atggtacaag
     2461 ttcatcgaag gaacaacccg taaacaagct gtggtgctaa atgaccgtgt gaagcaggtc
     2521 tctggtactc tcattattaa ggacgcagta gtcgaggact ccggaaaata cctgtgcgtg
     2581 gtgaacaatt ccgtgggagg cgaaagcgtt gagactgtgc ttaccgtcac tgccccccta
     2641 tccgctaaga tcgatcctcc cacacagaca gttgacttcg gacgccccgc cgtattcact
     2701 tgccagtaca ctggaaatcc catcaagacc gttagctgga tgaaggacgg caaggctatc
     2761 ggacacagcg aacccgtgct gcgcatcgaa tccgtgaaga aggaggacaa gggcatgtac
     2821 cagtgcttcg taagaaacga ccaggaatcg gcggaggcga gtgctgagct gaagctcgga
     2881 ggccgtttcg atccccccgt catccgacag gccttccagg aagagaccat ggaacccgga
     2941 ccaagtgtat tcctcaagtg cgtcgccggc ggaaacccca cgcccgaaat ttcctgggaa
     3001 ctcgacggca agaaaatcgc caacaacgat cgctatcagg tcggacagta tgtgacggtc
     3061 aacggagatg tggtttccta tctgaacata acctcggtcc acgccaacga tggcggtctc
     3121 tacaagtgca tagccaagag caaagtgggc gtggccgaac actcggccaa gttgaatgta
     3181 tatggactgc cctacatccg gcaaatggag aagaaggcaa ttgttgctgg agaaaccctg
     3241 attgtcacat gtcctgtagc tggatacccc atcgattcga ttgtctggga gcgagataac
     3301 cgtgccctgc ccatcaaccg caaacagaag gtcttcccca acggaacgct gattatcgag
     3361 aatgtggaac ggaactcaga ccaagcgacc tacacttgcg ttgccaagaa tcaggaagga
     3421 tactctgctc gaggatctct ggaagtgcaa gtcatggttc cacccaaaat tacgcccttc
     3481 gacttcggtg acgctccgac gaatgtggag gactctgttt cggtcatgtg cctgatttcc
     3541 aatggggatt tacccatcga catcgagtgg ttctttaacg actacggaat tagttcgtac
     3601 tccggaataa atgtagttaa aggtggcaag aggaacagca tgctaagtat tgatagtgtc
     3661 caggctcgtc atgccgggaa gtatagttgt cgggctaaga accacgctgc tgctgtaaat
     3721 tataccaccg aactaatagt taatgtacct cccagatgga tcctcgagcc caccgataag
     3781 gccttcgccc agggatccga tgccaaggtt gaatgcaagg ctgatggctt ccccaaaccc
     3841 caagttacat ggaagaaagc agttggcgac acccccggag agtacaagga tctaaagaag
     3901 agcgacaaca tccgcgtgga agagggcact ctgcatgtcg acaacatcca aaagaccaac
     3961 gagggctact atctgtgcga ggctatcaat ggaataggct ccggcctctc ggctgtgatt
     4021 atgatcagcg ttcaggcccc tccagagttc acggagaagc tgcgcaacca gaccgcccga
     4081 cgaggagaac ccgccgtact ccagtgcgag gcgaagggcg agaagcccat tggcatcttg
     4141 tggaacatga acaacatgcg actggaccca aagaacgaca atcggtacac cattcgtgag
     4201 gagatccttt ccaccggagt tatgtccagt ctgtcgatca agcgcaccga gagatccgac
     4261 tccgccctat tcacctgtgt cgccactaat gcctttggat ccgatgacgc cagcataaat
     4321 atgattgtcc aggaagttcc ggagatgcca tatgctttga aggtactcga caaatccgga
     4381 cgttccgtgc agctgagctg ggcgcaaccc tacgatggca actcccctct ggacaggtac
     4441 atcattgagt ttaagcgttc tcgtgcctcc tggagcgaaa ttgatcgcgt catcgtgccc
     4501 ggacacacca cagaagccca agtgcagaag ctcagccccg ccaccaccta caacattcgc
     4561 atcgtggccg aaaacgccat cggcacatcc caatcctctg aagcggtcac tatcatcact
     4621 agtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca
     4681 gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc
     4741 acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg
     4801 gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg
     4861 taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac
     4921 aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc
     4981 aaagggcagc cccgagaacc acaggtgtat accctgcccc catcccggga tgagctgacc
     5041 aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg
     5101 gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac
     5161 tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag
     5221 gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag
     5281 agcctctccc tgtctccggg taaaggcggc catcatcacc atcaccatta atctagagat
     5341 cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5461 catcaatgta tcttatcatg tctggtcgac tctagctaga ctcttccgct tcctcgctca
     5521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6721 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7321 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7381 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     7441 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     7501 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggaggaat
     7561 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7621 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7681 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7801 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7861 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7921 gtcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7981 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8041 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8101 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     8161 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     8221 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     8281 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     8341 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     8401 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     8461 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     8521 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     8581 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     8641 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     8701 acgacgttgt aaaacgacgg ccagtgaatt gtcgaggtct cgacggtatc gattgacatt
     8761 gattattgac ta
//
