LOCUS       sequence-232981-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.26 protein, C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.26 EC10-Fc-His
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        375..578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     529..549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     575..599
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          714..1539
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5319..5336
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5362..5483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5399..5418)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5453..5472
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5521..5538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5692..6280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5772..5791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6451..7242,7243..7311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7074..7093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7312..7416)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7484..7502
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7540..7562)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7564..7584)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7569..7898
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7749..7884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7811..7830
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8035..8054
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8121..8576)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8258..8279)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8470..8489
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8702..8724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8716..8733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8717..8733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(8763..8772,1..374)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attgactagc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
      601 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      661 tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta
      721 ccgcctatag actctatagg cacacccctt tggctcttat gcatgctata ctgtttttgg
      781 cttggggcct atacaccccc gcttccatat gctataggtg atggtatagc ttagcctata
      841 ggtgtgggtt attgaccatt attgaccact cccctattgg tgacgatact ttccattact
      901 aatccataac atggctcttt gccacaacta tctctattgg ctatatgcca atactctgtc
      961 cttcagagac tgacacggac tctgtatttt tacaggatgg ggtcccattt attatttaca
     1021 aattcacata tacaacaacg ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg
     1081 gatctccacg cgaatctcgg gtacgtgttc cggacatggg ctcttctccg gtagcggcgg
     1141 agcttccaca tccgagccct ggtcccatgc ctccagcggc tcatggtcgc tcggcagctc
     1201 cttgctccta acagtggagg ccagacttag gcacagcaca atgcccacca ccaccagtgt
     1261 gccgcacaag gccgtggcgg tagggtatgt gtctgaaaat gagctcggag attgggctcg
     1321 caccgctgac gcagatggaa gacttaaggc agcggcagaa gaagatgcag gcagctgagt
     1381 tgttgtattc tgataagagt cagaggtaac tcccgttgcg gtgctgttaa cggtggaggg
     1441 cagtgtagtc tgagcagtac tcgttgctgc cgcgcgcgcc accagacata atagctgaca
     1501 gactaacaga ctgttccttt ccatgggtct tttctgcaga agcttgaatt ctcgaggatc
     1561 cgcggccgct actgccaact tcacacaacc cgctcagcgg aaacacggca tacgaaatga
     1621 atatgcccaa cgaacgcctc aaatggctaa tgctgttcgc agctgttgcc ctcattgctt
     1681 gtggtagtca gaccctagct gccaatcccc cagatgccga ccaaaaagga cccgtcttcc
     1741 tcaaggaacc caccaaccgc attgacttct ccaactccac gggcgcagag atcgagtgca
     1801 aggccagcgg caatcccatg cccgagatta tttggatcag gagcgacggt accgccgtgg
     1861 gtgatgtgcc cggattgcgt cagatctcat ccgacggcaa gctggtcttc cctcccttcc
     1921 gcgccgagga ctaccgccag gaggtccatg cccaggtgta cgcctgcctg gcccgcaacc
     1981 agttcggatc cattatctcc cgggacgtcc atgtgcgagc cgttgtcaat cagttctatg
     2041 aggcggagat tatgaccgag tatgttatca ggggtaatgc tgccgttctg aagtgttcca
     2101 tcccgtcgtt cgtggctgat tttgttcgag ttgagtcctg gatcgatgat gagggcaatg
     2161 tcttgtcctt ttcggacaat tacgatggaa agtatctggt attgccctct ggagagctgc
     2221 acatccgtga ggttggaccc gaggacggat acaagagcta ccagtgccga accaaacatc
     2281 gtctgaccgg agaaacccga ttaagtgcca caaaaggacg attggtcatc acagaacctg
     2341 tgggcagtgt tgcgcctaag atcagcgtag aaaatcgtct gaaaaatgca gaggctcgaa
     2401 ttggttccac cttcaccctc ttttgccctg gtcaagccta tccagtaccc ttctttagat
     2461 ggtacaagtt catcgaagga acaacccgta aacaagctgt ggtgctaaat gaccgtgtga
     2521 agcaggtctc tggtactctc attattaagg acgcagtagt cgaggactcc ggaaaatacc
     2581 tgtgcgtggt gaacaattcc gtgggaggcg aaagcgttga gactgtgctt accgtcactg
     2641 cccccctatc cgctaagatc gatcctccca cacagacagt tgacttcgga cgccccgccg
     2701 tattcacttg ccagtacact ggaaatccca tcaagaccgt tagctggatg aaggacggca
     2761 aggctatcgg acacagcgaa cccgtgctgc gcatcgaatc cgtgaagaag gaggacaagg
     2821 gcatgtacca gtgcttcgta agaaacgacc aggaatcggc ggaggcgagt gctgagctga
     2881 agctcggagg ccgtttcgat ccccccgtca tccgacaggc cttccaggaa gagaccatgg
     2941 aacccggacc aagtgtattc ctcaagtgcg tcgccggcgg aaaccccacg cccgaaattt
     3001 cctgggaact cgacggcaag aaaatcgcca acaacgatcg ctatcaggtc ggacagtatg
     3061 tgacggtcaa cggagatgtg gtttcctatc tgaacataac ctcggtccac gccaacgatg
     3121 gcggtctcta caagtgcata gccaagagca aagtgggcgt ggccgaacac tcggccaagt
     3181 tgaatgtata tggactgccc tacatccggc aaatggagaa gaaggcaatt gttgctggag
     3241 aaaccctgat tgtcacatgt cctgtagctg gataccccat cgattcgatt gtctgggagc
     3301 gagataaccg tgccctgccc atcaaccgca aacagaaggt cttccccaac ggaacgctga
     3361 ttatcgagaa tgtggaacgg aactcagacc aagcgaccta cacttgcgtt gccaagaatc
     3421 aggaaggata ctctgctcga ggatctctgg aagtgcaagt catggttcct ccgaaaatca
     3481 cgccatttga cttcggtgcc gaacccacca atgttgagga ttccgtttcg gtgacttgtc
     3541 taatctcctc cggcgatttg cccattgata tcgagtggtt cttcaacgac tacggaatta
     3601 gctcctactc cggtataaat gtagttaagg gtggcaagag gaacagtatg ctgagcatcg
     3661 ataatgtgca ggctaggcat gcgggaaagt atagttgccg ggcaaagaac tacgcagcag
     3721 ccgtcaacta cagcactgaa ctcatagtta atgtacctcc cagatggatc ctcgagccca
     3781 ccgataaggc cttcgcccag ggatccgatg ccaaggttga atgcaaggct gatggcttcc
     3841 ccaaacccca agttacatgg aagaaagcag ttggcgacac ccccggagag tacaaggatc
     3901 taaagaagag cgacaacatc cgcgtggaag agggcactct gcatgtcgac aacatccaaa
     3961 agaccaacga gggctactat ctgtgcgagg ctatcaatgg aataggctcc ggcctctcgg
     4021 ctgtgattat gatcagcgtt caggcccctc cagagttcac ggagaagctg cgcaaccaga
     4081 ccgcccgacg aggagaaccc gccgtactcc agtgcgaggc gaagggcgag aagcccattg
     4141 gcatcttgtg gaacatgaac aacatgcgac tggacccaaa gaacgacaat cggtacacca
     4201 ttcgtgagga gatcctttcc accggagtta tgtccagtct gtcgatcaag cgcaccgaga
     4261 gatccgactc cgccctattc acctgtgtcg ccactaatgc ctttggatcc gatgacgcca
     4321 gcataaatat gattgtccag gaagttccgg agatgccata tgctttgaag gtactcgaca
     4381 aatccggacg ttccgtgcag ctgagctggg cgcaacccta cgatggcaac tcccctctgg
     4441 acaggtacat cattgagttt aagcgttctc gtgcctcctg gagcgaaatt gatcgcgtca
     4501 tcgtgcccgg acacaccaca gaagcccaag tgcagaagct cagccccgcc accacctaca
     4561 acattcgcat cgtggccgaa aacgccatcg gcacatccca atcctctgaa gcggtcacta
     4621 tcatcactag tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg
     4681 gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc
     4741 ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact
     4801 ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca
     4861 acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca
     4921 aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct
     4981 ccaaagccaa agggcagccc cgagaaccac aggtgtatac cctgccccca tcccgggatg
     5041 agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca
     5101 tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg
     5161 tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt
     5221 ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac aaccactaca
     5281 cgcagaagag cctctccctg tctccgggta aaggcggcca tcatcaccat caccattaat
     5341 ctagagatct tattaaagca gaacttgttt attgcagctt ataatggtta caaataaagc
     5401 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5461 tccaaactca tcaatgtatc ttatcatgtc tggtcgactc tagctagact cttccgcttc
     5521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7441 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7501 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7561 ggaggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7621 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7741 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7801 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7861 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7921 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7981 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     8041 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     8101 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8161 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8221 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8281 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8341 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8401 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8461 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8521 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     8581 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8641 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8701 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt cgaggtctcg acggtatcga
     8761 ttgacattga tt
//
