LOCUS       sequence-23299-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human SUMO2 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET
  TITLE     Covalent modification of PML by the sentrin family of ubiquitin-like
            proteins.
  JOURNAL   J Biol Chem. 1998 Feb 6. 273(6):3117-20.
  PUBMED    9452416
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Feb 6. 273(6):3117-20."
COMMENT     Sequence Label: SRa-HA-SUMO2 (Sentrin 3)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..342)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     CDS             complement(352..378)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(396..414)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(397..414)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          518..614
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 aacaagttct gctttaataa gatctggatc ccccgggctg cagcccaagc taattcagta
       61 gacacctccc gtctgctgtt ggaacacatc aattgtatct tcatcctcca tttccaactg
      121 tgcaggtgtg tctgtttcat tgattggttg cccgtcaaat cggaatctga tctgcctcat
      181 tgacaatccc tgtcgttcac aataggcttt cattagttta ctaagtggtg tatgcctctt
      241 aatcttaaac tgcaccacag aaccatcctg ccccgccacc ttcaaattaa tatgatcgtt
      301 gttctcagtc ttgactcctt ccttgggctt ttcgtcggcc atggatccgc tagcgtaatc
      361 cggaacatcg tatgggtaca taagctagct tgtattctat agtgtcacct aaatcgtatg
      421 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgaattcc
      481 gcagctttta gagcagaagt aacacttccg tacaggccta gaagtaaagg caacatccac
      541 tgaggagcag ttctttgatt tgcaccacca ccggatccgg gacctgaaat aaaagacaaa
      601 aagactaaac ttaccagtta actttctggt ttttcagttc ctcgaggagc ttggatctgt
      661 aacggcgcag aacagaaaac gaaacaaaga cgtagagttg agcaagcagg gtcaggcaaa
      721 gcgtggagag ccggctgagt ctaggtaggc tccaagggag cgccggacaa aggcccggtc
      781 tcgacctgag ctttaaactt acctagacgg cggacgcagt tc
//
