LOCUS       sequence-233005-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.30 protein, C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.30 EC10-Fc-His
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        343..546
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     497..517
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     543..567
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          682..1507
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5287..5304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5330..5451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5367..5386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5421..5440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5489..5506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5660..6248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5740..5759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6419..7210,7211..7279))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7042..7061
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7280..7384)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7452..7470
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7508..7530)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7532..7552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7537..7866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7717..7852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7779..7798
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8003..8022
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8089..8544)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8226..8247)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8438..8457
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8670..8692
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8684..8701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8685..8701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(8731..8772,1..342)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
       61 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      121 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      181 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      241 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      301 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      361 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      421 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      481 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      541 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      601 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      661 attccccgtg ccaagagtga cgtaagtacc gcctatagac tctataggca cacccctttg
      721 gctcttatgc atgctatact gtttttggct tggggcctat acacccccgc ttccatatgc
      781 tataggtgat ggtatagctt agcctatagg tgtgggttat tgaccattat tgaccactcc
      841 cctattggtg acgatacttt ccattactaa tccataacat ggctctttgc cacaactatc
      901 tctattggct atatgccaat actctgtcct tcagagactg acacggactc tgtattttta
      961 caggatgggg tcccatttat tatttacaaa ttcacatata caacaacgcc gtcccccgtg
     1021 cccgcagttt ttattaaaca tagcgtggga tctccacgcg aatctcgggt acgtgttccg
     1081 gacatgggct cttctccggt agcggcggag cttccacatc cgagccctgg tcccatgcct
     1141 ccagcggctc atggtcgctc ggcagctcct tgctcctaac agtggaggcc agacttaggc
     1201 acagcacaat gcccaccacc accagtgtgc cgcacaaggc cgtggcggta gggtatgtgt
     1261 ctgaaaatga gctcggagat tgggctcgca ccgctgacgc agatggaaga cttaaggcag
     1321 cggcagaaga agatgcaggc agctgagttg ttgtattctg ataagagtca gaggtaactc
     1381 ccgttgcggt gctgttaacg gtggagggca gtgtagtctg agcagtactc gttgctgccg
     1441 cgcgcgccac cagacataat agctgacaga ctaacagact gttcctttcc atgggtcttt
     1501 tctgcagaag cttgaattct cgaggatccg cggccgctac tgccaacttc acacaacccg
     1561 ctcagcggaa acacggcata cgaaatgaat atgcccaacg aacgcctcaa atggctaatg
     1621 ctgttcgcag ctgttgccct cattgcttgt ggtagtcaga ccctagctgc caatccccca
     1681 gatgccgacc aaaaaggacc cgtcttcctc aaggaaccca ccaaccgcat tgacttctcc
     1741 aactccacgg gcgcagagat cgagtgcaag gccagcggca atcccatgcc cgagattatt
     1801 tggatcagga gcgacggtac cgccgtgggt gatgtgcccg gattgcgtca gatctcatcc
     1861 gacggcaagc tggtcttccc tcccttccgc gccgaggact accgccagga ggtccatgcc
     1921 caggtgtacg cctgcctggc ccgcaaccag ttcggatcca ttatctcccg ggacgtccat
     1981 gtgcgagccg ttgtcaatca gttctatgag gcggagatta tgaccgagta tgttatcagg
     2041 ggtaatgctg ccgttctgaa gtgttccatc ccgtcgttcg tggctgattt tgttcgagtt
     2101 gagtcctgga tcgatgatga gggcaatgtc ttgtcctttt cggacaatta cgatggaaag
     2161 tatctggtat tgccctctgg agagctccac atccgtgagg ttggacccga ggacggatac
     2221 aagagctacc agtgccgaac caaacatcgt ctgaccggag aaacccgatt aagtgccaca
     2281 aaaggacgat tggtcatcac agaacctgtg ggcagtgttg cgcctaagat cagcgtagaa
     2341 aatcgtctga aaaatgcaga ggctcgaatt ggttccacct tcaccctctt ttgccctggt
     2401 caagcctatc cagtaccctt ctttagatgg tacaagttca tcgaaggaac aacccgtaaa
     2461 caagctgtgg tgctaaatga ccgtgtgaag caggtctctg gtactctcat tattaaggac
     2521 gcagtagtcg aggactccgg aaaatacctg tgcgtggtga acaattccgt gggaggcgaa
     2581 agcgttgaga ctgtgcttac cgtcactgcc cccctatccg ctaagatcga tcctcccaca
     2641 cagacagttg acttcggacg ccccgccgta ttcacttgcc agtacactgg aaatcccatc
     2701 aagaccgtta gctggatgaa ggacggcaag gctatcggac acagcgaacc cgtgctgcgc
     2761 atcgaatccg tgaagaagga ggacaagggc atgtaccagt gcttcgtaag aaacgaccag
     2821 gaatcggcgg aggcgagtgc tgagctgaag ctcggaggcc gtttcgatcc ccccgtcatc
     2881 cgacaggcct tccaggaaga gaccatggaa cccggaccaa gtgtattcct caagtgcgtc
     2941 gccggcggaa accccacgcc cgaaatttcc tgggaactcg acggcaagaa aatcgccaac
     3001 aacgatcgct atcaggtcgg acagtatgtg acggtcaacg gagatgtggt ttcctatctg
     3061 aacataacct cggtccacgc caacgatggc ggtctctaca agtgcatagc caagagcaaa
     3121 gtgggcgtgg ccgaacactc ggccaagttg aatgtatatg gactgcccta catccggcaa
     3181 atggagaaga aggcaattgt tgctggagaa accctgattg tcacatgtcc tgtagctgga
     3241 taccccatcg attcgattgt ctgggagcga gataaccgtg ccctgcccat caaccgcaaa
     3301 cagaaggtct tccccaacgg aacgctgatt atcgagaatg tggaacggaa ctcagaccaa
     3361 gcgacctaca cttgcgttgc caagaatcag gaaggatact ctgctcgagg atctctggaa
     3421 gtgcaagtca tggttcttcc gcgcatcata cccttcgcct tcgaggaggg tcctgcccag
     3481 gtgggtcagt acctgacact gcactgctcc gttcccggcg gagatctgcc cctgaacatc
     3541 gactggacgc tggatggtca ggcaatctcg gaggatctgg gaataaccac gtcccgcgtg
     3601 ggtcgtcgtg gaagtgtgct aaccatcgag gccgtggagg cctcacatgc gggcaacttc
     3661 acctgccacg cccgcaattt ggcgggccat cagcagttca ccacgccgct gaacgtctac
     3721 gtacctccca gatggatcct cgagcccacc gataaggcct tcgcccaggg atccgatgcc
     3781 aaggttgaat gcaaggctga tggcttcccc aaaccccaag ttacatggaa gaaagcagtt
     3841 ggcgacaccc ccggagagta caaggatcta aagaagagcg acaacatccg cgtggaagag
     3901 ggcactctgc atgtcgacaa catccaaaag accaacgagg gctactatct gtgcgaggct
     3961 atcaatggaa taggctccgg cctctcggct gtgattatga tcagcgttca ggcccctcca
     4021 gagttcacgg agaagctgcg caaccagacc gcccgacgag gagaacccgc cgtactccag
     4081 tgcgaggcga agggcgagaa gcccattggc atcttgtgga acatgaacaa catgcgactg
     4141 gacccaaaga acgacaatcg gtacaccatt cgtgaggaga tcctttccac cggagttatg
     4201 tccagtctgt cgatcaagcg caccgagaga tccgactccg ccctattcac ctgtgtcgcc
     4261 actaatgcct ttggatccga tgacgccagc ataaatatga ttgtccagga agttccggag
     4321 atgccatatg ctttgaaggt actcgacaaa tccggacgtt ccgtgcagct gagctgggcg
     4381 caaccctacg atggcaactc ccctctggac aggtacatca ttgagtttaa gcgttctcgt
     4441 gcctcctgga gcgaaattga tcgcgtcatc gtgcccggac acaccacaga agcccaagtg
     4501 cagaagctca gccccgccac cacctacaac attcgcatcg tggccgaaaa cgccatcggc
     4561 acatcccaat cctctgaagc ggtcactatc atcactagtg acaaaactca cacatgccca
     4621 ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc
     4681 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
     4741 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
     4801 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc
     4861 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc
     4921 ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag
     4981 gtgtataccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc
     5041 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
     5101 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac
     5161 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg
     5221 atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa
     5281 ggcggccatc atcaccatca ccattaatct agagatctta ttaaagcaga acttgtttat
     5341 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5401 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5461 gtcgactcta gctagactct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5521 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5641 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5761 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5821 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5881 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5941 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6001 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6061 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6121 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6181 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6241 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6301 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6361 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6421 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6481 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6541 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6601 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6661 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6721 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6781 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6841 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6901 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6961 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7021 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7081 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7141 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7201 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7261 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7321 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7381 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     7441 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     7501 gtgaaaacct ctgacacatg cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag
     7561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7621 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7801 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7861 tgcaaaaagc tcccgggagc ttgtatatcc attttcgtcc cggagacggt cacagcttgt
     7921 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7981 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     8041 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     8101 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     8161 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     8221 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     8281 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     8341 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     8401 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     8461 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     8521 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     8581 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     8641 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     8701 tgaattgtcg aggtctcgac ggtatcgatt gacattgatt attgactagc tagttattaa
     8761 tagtaatcaa tt
//
