LOCUS       sequence-233044-        7844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of LIR mutant NDP52 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Padman BS, Nguyen TN, Uoselis L, Skulsuppaisarn M, Nguyen LK, 
            Lazarou M
  TITLE     LC3/GABARAPs drive ubiquitin-independent recruitment of Optineurin 
            and NDP52 to amplify mitophagy.
  JOURNAL   Nat Commun. 2019 Jan 24;10(1):408. doi: 10.1038/s41467-019-08335-6.
  PUBMED    30679426
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-24"; volume: "10(1):408. doi"; pages: " 
            10.1038/s41467-019-08335-6"
COMMENT     Sequence Label: pBMN mEGFP-NDP52(V136S)
FEATURES             Location/Qualifiers
     source          1..7844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             348..817
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    882..1239
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1304..1720
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1650..1672
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1688..1704
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1730..2104
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2042..2058
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2107..2116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2113..2115,2116..2118,2119..2829)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2158..2179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2419..2438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2766..2787
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4391..4860
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4990..5007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5161..5749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5241..5260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5913..6704,6705..6773))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6536..6555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6774..6878)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  7023..7790
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata aaaatagata
       61 aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt gacaacataa
      121 atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta tgccagtcat
      181 attaattact agtcaattag ttgattttta tttttgacat atacatgtga aagaccccac
      241 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
      301 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      361 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      421 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      481 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      541 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      601 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      661 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      721 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      781 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      841 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      901 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      961 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
     1021 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
     1081 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
     1141 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1201 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1261 ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac
     1321 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
     1381 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
     1441 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
     1501 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     1561 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     1621 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     1681 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
     1741 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
     1801 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
     1861 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
     1921 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1981 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     2041 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     2101 actgccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2161 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2221 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2281 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2341 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2401 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2461 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2521 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2581 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2641 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2701 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     2761 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2821 ctgtacaagt ccggactcag atctcgagct caagcttggg aggagaccat caaagatccc
     2881 cccacatcag ctgtcttgct ggatcactgt catttctctc aggtcatctt taacagtgtg
     2941 gagaagttct acatccctgg aggggacgtc acatgtcatt ataccttcac ccagcatttc
     3001 atccctcgtc gaaaggattg gattggcatc tttagagtgg ggtggaagac aacccgtgag
     3061 tattacacct tcatgtgggt tactttgccc attgacctaa acaacaaatc agctaaacag
     3121 caggaagtcc aattcaaagc ttactacctg cccaaggatg atgagtatta ccagttctgc
     3181 tatgtggatg aggatggtgt ggtccgggga gcaagtattc ctttccaatt ccgtccagaa
     3241 aatgaggaag acatcctggt tagtaccact cagggagagg tggaagagat tgagcagcac
     3301 aacaaggagc tttgcaaaga aaaccaggag ctgaaggaca gctgtatcag cctccagaag
     3361 cagaactcag acatgcaggc tgagctccaa aagaagcagg aggagctaga aaccctacag
     3421 agcatcaata agaagttgga actgaaagtg aaagaacaga aggactattg ggagacagag
     3481 ctgcttcaac tgaaagaaca aaaccagaag atgtcctcag aaaatgagaa gatgggaatc
     3541 agagtggatc agcttcaggc ccagctgtca actcaagaga aagaaatgga gaagcttgtt
     3601 cagggagatc aagataagac agagcagtta gagcagctga aaaaggaaaa tgaccacctc
     3661 tttctcagtt tagctgaaca gaggaaggac cagaagaagc tcgagcagac agtggagcaa
     3721 atgaagcaga atgaaactac tgcaatgaag aaacaacagg aattaatgga tgaaaacttt
     3781 gacctgtcaa aaagactgag tgagaacgaa attatatgta atgctctgca gagacagaaa
     3841 gagagattgg aaggagaaaa tgatcttttg aagagggaga acagcagatt gctcagttac
     3901 atgggtctgg attttaattc tttgccgtat caagtaccta cttcagatga aggaggcgca
     3961 agacaaaatc caggacttgc ctatggaaac ccatattctg gtatccaaga aagttcttcc
     4021 cccagcccgc tctccatcaa gaaatgccct atctgcaaag cagatgatat ttgtgatcac
     4081 accttggagc aacagcagat gcagcccctt tgtttcaatt gtccaatttg tgacaagatc
     4141 ttcccagcta cagagaagca gatctttgaa gaccacgtgt tctgccactc tctctgagga
     4201 tccaccggat ctagataact gatcacgata aaataaaaga ttttatttag tctccagaaa
     4261 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     4321 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
     4381 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     4441 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     4501 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     4561 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     4621 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     4681 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     4741 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     4801 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     4861 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     4921 atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4981 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5221 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5281 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5341 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5401 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5461 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5521 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5581 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5641 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5761 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5821 catgagatta tcaaaaagga tcttcaccta gatccttttg cggccggccg caaatcaatc
     5881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6901 ccagctttgc tcttaggagt ttcctaatac atcccaaact caaatatata aagcatttga
     6961 cttgttctat gccctagggg gcggggggaa gctaagccag ctttttttaa catttaaaat
     7021 gtggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg
     7081 tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc
     7141 tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc
     7201 agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg
     7261 tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat
     7321 acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc
     7381 gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg
     7441 cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg
     7501 tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca
     7561 tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc
     7621 atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac
     7681 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag
     7741 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc ttaaaatgtt
     7801 aattccattt taaatgcaca gatgttttta tttcataagg gttt
//
