LOCUS       sequence-233053-       12187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stably express BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Li Q, Li Y, Yang S, Huang S, Yan M, Ding Y, Tang W, Lou X, Yin Q, 
            Sun Z, Lu L, Shi H, Wang H, Chen Y, Li J
  TITLE     CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 
            amino acids that are critical for primordial germ cell development.
  JOURNAL   Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 
            10.1038/s41556-018-0202-4.
  PUBMED    30275529
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 
            10.1038/s41556-018-0202-4."
COMMENT     Sequence Label: PB-BE3-NLS
FEATURES             Location/Qualifiers
     source          1..12187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             59..733
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             59..733
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(189..207)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     457..476
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     651..674
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             737..790
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             797..1483
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1532..4930,4931..4933,4934..5530,5531,5532..5534,
                     5535..5536,5537..5539,5540..5632)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1532..1555,1556..1558,1559..5632)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5645..5893
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5906..5926
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5935..5952
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6079..6098)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6144..6199
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6198..6217)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6560..6576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6584..6600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6608..6614,6615..6632,6633..6638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6653..6674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7375..7409
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7582..7602)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7582..7600)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7621..7637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7645..7661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7669..7675,7676..7693,7694..7699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7714..7735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7852..7869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8023..8611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8103..8122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8782..9573,9574..9642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9405..9424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9643..9747)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9774..10229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9861..9880)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10071..10092
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10355..10377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10369..10386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10370..10386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10396..10415
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10396..10414
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   10662..10724
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        11808..12187
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgatccgat
       61 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
      121 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      181 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
      241 gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      301 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      361 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      421 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      481 gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      541 caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta
      601 catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat
      661 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca
      721 ctccaccggc gcccccgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
      781 tcctggccca acgcgtatga gctcagagac tggcccagtg gctgtggacc ccacattgag
      841 acggcggatc gagccccatg agtttgaggt attcttcgat ccgagagagc tccgcaagga
      901 gacctgcctg ctttacgaaa ttaattgggg gggccggcac tccatttggc gacatacatc
      961 acagaacact aacaagcacg tcgaagtcaa cttcatcgag aagttcacga cagaaagata
     1021 tttctgtccg aacacaaggt gcagcattac ctggtttctc agctggagcc catgcggcga
     1081 atgtagtagg gccatcactg aattcctgtc aaggtatccc cacgtcactc tgtttattta
     1141 catcgcaagg ctgtaccacc acgctgaccc ccgcaatcga caaggcctgc gggatttgat
     1201 ctcttcaggt gtgactatcc aaattatgac tgagcaggag tcaggatact gctggagaaa
     1261 ctttgtgaat tatagcccga gtaatgaagc ccactggcct aggtatcccc atctgtgggt
     1321 acgactgtac gttcttgaac tgtactgcat catactgggc ctgcctcctt gtctcaacat
     1381 tctgagaagg aagcagccac agctgacatt ctttaccatc gctcttcagt cttgtcatta
     1441 ccagcgactg cccccacaca ttctctgggc caccgggttg aaaagcggca gcgagactcc
     1501 cgggacctca gagtccgcca cacccgaaag tgataaaaag tattctattg gtttagccat
     1561 cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa
     1621 atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct
     1681 cctattcgat agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag
     1741 gtatacacgt cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc
     1801 caaagttgac gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa
     1861 gaaacatgaa cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa
     1921 gtacccaacg atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct
     1981 gaggttaatc tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga
     2041 gggtgatcta aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac
     2101 ctataatcag ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat
     2161 tcttagcgcc cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg
     2221 agagaagaaa aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa
     2281 ttttaagtcg aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta
     2341 cgatgacgat ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt
     2401 ggctgccaaa aaccttagcg atgcaatcct cctatctgac atactgagag ttaatactga
     2461 gattaccaag gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga
     2521 cttgacactt ctcaaggccc tagtccgtca gcaactgcct gagaaatata aggaaatatt
     2581 ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga gtcaagagga
     2641 attctacaag tttatcaaac ccatattaga gaagatggat gggacggaag agttgcttgt
     2701 aaaactcaat cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg gtagcattcc
     2761 acatcaaatc cacttaggcg aattgcatgc tatacttaga aggcaggagg atttttatcc
     2821 gttcctcaaa gacaatcgtg aaaagattga gaaaatccta acctttcgca taccttacta
     2881 tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg atgacaagaa agtccgaaga
     2941 aacgattact ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt
     3001 catcgagagg atgaccaact ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca
     3061 cagtttactt tacgagtatt tcacagtgta caatgaactc acgaaagtta agtatgtcac
     3121 tgagggcatg cgtaaacccg cctttctaag cggagaacag aagaaagcaa tagtagatct
     3181 gttattcaag accaaccgca aagtgacagt taagcaattg aaagaggact actttaagaa
     3241 aattgaatgc ttcgattctg tcgagatctc cggggtagaa gatcgattta atgcgtcact
     3301 tggtacgtat catgacctcc taaagataat taaagataag gacttcctgg ataacgaaga
     3361 gaatgaagat atcttagaag atatagtgtt gactcttacc ctctttgaag atcgggaaat
     3421 gattgaggaa agactaaaaa catacgctca cctgttcgac gataaggtta tgaaacagtt
     3481 aaagaggcgt cgctatacgg gctggggacg attgtcgcgg aaacttatca acgggataag
     3541 agacaagcaa agtggtaaaa ctattctcga ttttctaaag agcgacggct tcgccaatag
     3601 gaactttatg cagctgatcc atgatgactc tttaaccttc aaagaggata tacaaaaggc
     3661 acaggtttcc ggacaagggg actcattgca cgaacatatt gcgaatcttg ctggttcgcc
     3721 agccatcaaa aagggcatac tccagacagt caaagtagtg gatgagctag ttaaggtcat
     3781 gggacgtcac aaaccggaaa acattgtaat cgagatggca cgcgaaaatc aaacgactca
     3841 gaaggggcaa aaaaacagtc gagagcggat gaagagaata gaagagggta ttaaagaact
     3901 gggcagccag atcttaaagg agcatcctgt ggaaaatacc caattgcaga acgagaaact
     3961 ttacctctat tacctacaaa atggaaggga catgtatgtt gatcaggaac tggacataaa
     4021 ccgtttatct gattacgacg tcgatcacat tgtaccccaa tcctttttga aggacgattc
     4081 aatcgacaat aaagtgctta cacgctcgga taagaaccga gggaaaagtg acaatgttcc
     4141 aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa atgcgaaact
     4201 gataacgcaa agaaagttcg ataacttaac taaagctgag aggggtggct tgtctgaact
     4261 tgacaaggcc ggatttatta aacgtcagct cgtggaaacc cgccaaatca caaagcatgt
     4321 tgcacagata ctagattccc gaatgaatac gaaatacgac gagaacgata agctgattcg
     4381 ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg gacttcagaa aggattttca
     4441 attctataaa gttagggaga taaataacta ccaccatgcg cacgacgctt atcttaatgc
     4501 cgtcgtaggg accgcactca ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg
     4561 tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg agataggcaa
     4621 ggctacagcc aaatacttct tttattctaa cattatgaat ttctttaaga cggaaatcac
     4681 tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga
     4741 aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca
     4801 agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct
     4861 tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta
     4921 cggtggcttc gatagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa
     4981 gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg
     5041 ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa
     5101 aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa
     5161 acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa
     5221 atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga
     5281 taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga
     5341 gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt
     5401 aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca
     5461 tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat
     5521 agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc
     5581 catcacggga ttatatgaaa ctcggataga tttgtcacag cttgggggtg actctggtgg
     5641 ttctactaat ctgtcagata ttattgaaaa ggagaccggt aagcaactgg ttatccagga
     5701 atccatcctc atgctcccag aggaggtgga agaagtcatt gggaacaagc cggaaagcga
     5761 tatactcgtg cacaccgcct acgacgagag caccgacgag aatgtcatgc ttctgactag
     5821 cgacgcccct gaatacaagc cttgggctct ggtcatacag gatagcaacg gtgagaacaa
     5881 gattaagatg ctctctggtg gttctcccaa gaagaagagg aaagtctaac cggtcatcat
     5941 caccatcacc attgagttta aacccgctga tcagcacgcg tgctagcttc gaagctaatt
     6001 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     6061 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     6121 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     6181 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     6241 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     6301 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     6361 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     6421 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     6481 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     6541 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6721 gccagcggat cgatctgaca atgttcagtg cagagactcg gctacgcctc gtggactttg
     6781 aagttgacca acaatgttta ttcttacctc taatagtcct ctgtggcaag gtcaagattc
     6841 tgttagaagc caatgaagaa cctggttgtt caataacatt ttgttcgtct aatatttcac
     6901 taccgcttga cgttggctgc acttcatgta cctcatctat aaacgcttct tctgtatcgc
     6961 tctggacgtc atcttcactt acgtgatctg atatttcact gtcagaatcc tcaccaacaa
     7021 gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa gaactaccca
     7081 ttttattata tattagtcac gatatctata acaagaaaat atatatataa taagttatca
     7141 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     7201 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     7261 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     7321 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     7381 tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta ctcgacctaa
     7441 actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg
     7501 acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga gcgtcgacct cgaggggggg
     7561 cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     7621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8461 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8581 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8701 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8761 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8821 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8881 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8941 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9001 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9061 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9121 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9181 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9241 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9301 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9361 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9421 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9481 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9541 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9601 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9661 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9721 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     9781 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9841 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9901 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9961 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
    10021 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    10081 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
    10141 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    10201 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
    10261 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10321 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10381 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggccgc
    10441 ccggtttatc gttaatatgg atcaatttga acagttgatt aacgtgtctc tgctcaagtc
    10501 tttgatcaaa acgcaaatcg acgaaaatgt gtcggacaat atcaagtcga tgagcgaaaa
    10561 actaaaaagg ctagaatacg acaatctcac agacagcgtt gagatatacg gtattcacga
    10621 cagcaggctg aataataaaa aaattagaaa ctattattta accctagaaa gataatcata
    10681 ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt
    10741 atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca
    10801 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca
    10861 aaaactcaaa atttcttcta taaagtaaca aaacttttaa acattctctc ttttacaaaa
    10921 ataaacttat tttgtacttt aaaaacagtc atgttgtatt ataaaataag taattagctt
    10981 aacttataca taatagaaac aaattatact tattagtcag tcagaaacaa ctttggcaca
    11041 tatcaatatt atgctctcga caaataactt ttttgcattt tttgcacgat gcatttgcct
    11101 ttcgccttat tttagagggg cagtaagtac agtaagtacg ttttttcatt actggctctt
    11161 cagtactgtc atctgatgta ccaggcactt catttggcaa aatattagag atattatcgc
    11221 gcaaatatct cttcaaagta ggagcttcta aacgcttacg cataaacgat gacgtcaggc
    11281 tcatgtaaag gtttctcata aattttttgc gactttgaac cttttctccc ttgctactga
    11341 cattatggct gtatataata aaagaattta tgcaggcaat gtttatcatt ccgtacaata
    11401 atgccatagg ccacctattc gtcttcctac tgcaggtcat cacagaacac atttggtcta
    11461 gcgtgtccac tccgccttta gtttgattat aatacataac catttgcggt ttaccggtac
    11521 tttcgttgat agaagcatcc tcatcacaag atgataataa gtataccatc ttagctggct
    11581 tcggtttata tgagacgaga gtaaggggtc cgtcaaaaca aaacatcgat gttcccactg
    11641 gcctggagcg actgtttttc agtacttccg gtatctcgcg tttgtttgat cgcacggttc
    11701 ccacaatggt taattcgagc tcgcccaaac cgggcgcgcc gggcatttaa atggaattcg
    11761 atgccctata tatggatcca attgcaatga tcatcatgac agatctcgac attgattatt
    11821 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    11881 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11941 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    12001 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    12061 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    12121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    12181 taccatg
//
