LOCUS       sequence-233071-       12805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a fusion protein of human BRCA1 with human 
            Rad18 Ubiquitin binding domain and BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 12805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-BRCA1-Rad18UBD
FEATURES             Location/Qualifiers
     source          1..12805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        38..417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..696
                     /label=chicken beta-actin promoter
     intron          697..1713
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1637..1659
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        1726..1744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1749..1758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7396..7414
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7416..7425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7425..7448
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7619..7635)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7655..7879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          8100..9038
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8995..9015
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      9073..9082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9079..9081,9082..9084,9085..9798)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9124..9145)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    9829..9940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10058..10075)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10058..10074)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10067..10089)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10215..10670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10352..10373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10564..10583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10697..10801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10802..10870,10871..11662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11020..11039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11833..12421
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12322..12341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12575..12592
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12709..12730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12745..12750,12751..12768,12769..12775)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12783..12799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(12788..12805,1..5)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 acaggaaaca gctatgacca tgaggcgcgc cggattcgac attgattatt gactagttat
       61 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      121 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      301 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      361 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      421 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      481 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      541 cgcgcgccrg gsggggcggg gcggggsgrg gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      721 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      781 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      841 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      901 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      961 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1021 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1081 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1141 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     1201 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     1261 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc
     1321 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1381 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1441 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1501 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1561 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1621 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1681 aaccatgttc atgccttctt ctttttccta cagatcctta attaataata cgactcacta
     1741 taggggccgc caccatggat ttatctgctc ttcgcgttga agaagtacaa aatgtcatta
     1801 atgctatgca gaaaatctta gagtgtccca tctgtctgga gttgatcaag gaacctgtct
     1861 ccacaaagtg tgaccacata ttttgcaaat tttgcatgct gaaacttctc aaccagaaga
     1921 aagggccttc acagtgtcct ttatgtaaga atgatataac caaaaggagc ctacaagaaa
     1981 gtacgagatt tagtcaactt gttgaagagc tattgaaaat catttgtgct tttcagcttg
     2041 acacaggttt ggagtatgca aacagctata attttgcaaa aaaggaaaat aactctcctg
     2101 aacatctaaa agatgaagtt tctatcatcc aaagtatggg ctacagaaac cgtgccaaaa
     2161 gacttctaca gagtgaaccc gaaaatcctt ccttgcagga aaccagtctc agtgtccaac
     2221 tctctaacct tggaactgtg agaactctga ggacaaagca gcggatacaa cctcaaaaga
     2281 cgtctgtcta cattgaattg ggatctgatt cttctgaaga taccgttaat aaggcaactt
     2341 attgcagtgt gggagatcaa gaattgttac aaatcacccc tcaaggaacc agggatgaaa
     2401 tcagtttgga ttctgcaaaa aaggctgctt gtgaattttc tgagacggat gtaacaaata
     2461 ctgaacatca tcaacccagt aataatgatt tgaacaccac tgagaagcgt gcagctgaga
     2521 ggcatccaga aaagtatcag ggtagttctg tttcaaactt gcatgtggag ccatgtggca
     2581 caaatactca tgccagctca ttacagcatg agaacagcag tttattactc actaaagaca
     2641 gaatgaatgt agaaaaggct gaattctgta ataaaagcaa acagcctggc ttagcaagga
     2701 gccaacataa cagatgggct ggaagtaagg aaacatgtaa tgataggcgg actcccagca
     2761 cagaaaaaaa ggtagatctg aatgctgatc ccctgtgtga gagaaaagaa tggaataagc
     2821 agaaactgcc atgctcagag aatcctagag atactgaaga tgttccttgg ataacactaa
     2881 atagcagcat tcagaaagtt aatgagtggt tttccagaag tgatgaactg ttaggttctg
     2941 atgactcaca tgatggggag tctgaatcaa atgccaaagt agctgatgta ttggacgttc
     3001 taaatgaggt agatgaatat tctggttctt cagagaaaat agacttactg gccagtgatc
     3061 ctcatgaggc tttaatatgt aaaagtgaaa gagttcactc caaatcagta gagagtaata
     3121 ttgaagacaa aatatttggg aaaacctatc ggaagaaggc aagcctcccc aacttaagcc
     3181 atgtaactga aaatctaatt ataggagcat ttgttactga gccacagata atacaagagc
     3241 gtcccctcac aaataaatta aagcgtaaaa ggagacctac atcaggcctt catcctgagg
     3301 attttatcaa gaaagcagat ttggcagttc aaaagactcc tgaaatgata aatcagggaa
     3361 ctaaccaaac ggagcagaat ggtcaagtga tgaatattac taatagtggt catgagaata
     3421 aaacaaaagg tgattctatt cagaatgaga aaaatcctaa cccaatagaa tcactcgaaa
     3481 aagaatctgc tttcaaaacg aaagctgaac ctataagcag cagtataagc aatatggaac
     3541 tcgaattaaa tatccacaat tcaaaagcac ctaaaaagaa taggctgagg aggaagtctt
     3601 ctaccaggca tattcatgcg cttgaactag tagtcagtag aaatctaagc ccacctaatt
     3661 gtactgaatt gcaaattgat agttgttcta gcagtgaaga gataaagaaa aaaaagtaca
     3721 accaaatgcc agtcaggcac agcagaaacc tacaactcat ggaaggtaaa gaacctgcaa
     3781 ctggagccaa gaagagtaac aagccaaatg aacagacaag taaaagacat gacagtgata
     3841 ctttcccaga gctgaagtta acaaatgcac ctggttcttt tactaagtgt tcaaatacca
     3901 gtgaacttaa agaatttgtc aatcctagcc ttccaagaga agaaaaagaa gagaaactag
     3961 aaacagttaa agtgtctaat aatgctgaag accccaaaga tctcatgtta agtggagaaa
     4021 gggttttgca aactgaaaga tctgtagaga gtagcagtat ttcactggta cctggtactg
     4081 attatggcac tcaggaaagt atctcgttac tggaagttag cactctaggg aaggcaaaaa
     4141 cagaaccaaa taaatgtgtg agtcagtgtg cagcatttga aaaccccaag ggactaattc
     4201 atggttgttc caaagataat agaaatgaca cagaaggctt taagtatcca ttgggacatg
     4261 aagttaacca cagtcgggaa acaagcatag aaatggaaga aagtgaactt gatgctcagt
     4321 atttgcagaa tacattcaag gtttcaaagc gccagtcatt tgctctgttt tcaaatccag
     4381 gaaatgcaga agaggaatgt gcaacattct ctgcccactc tgggtcctta aagaaacaaa
     4441 gtccaaaagt cacttttgaa tgtgaacaaa aggaagaaaa tcaaggaaag aatgagtcta
     4501 atatcaagcc tgtacagaca gttaatatca ctgcaggctt tcctgtggtt ggtcagaaag
     4561 ataagccagt tgataatgcc aaatgtagta tcaaaggagg ctctaggttt tgtctatcat
     4621 ctcagttcag aggcaacgaa actggactca ttactccaaa taaacatgga cttttacaaa
     4681 acccatatcg tataccacca ctttttccca tcaagtcatt tgttaaaact aaatgtaaga
     4741 aaaatctgct agaggaaaac tttgaggaac attcaatgtc acctgaaaga gaaatgggaa
     4801 atgagaacat tccaagtaca gtgagcacaa ttagccgtaa taacattaga gaaaatgttt
     4861 ttaaaggagc cagctcaagc aatattaatg aagtaggttc cagtactaat gaagtgggct
     4921 ccagtattaa tgaaataggt tccagtgatg aaaacattca agcagaacta ggtagaaaca
     4981 gagggccaaa attgaatgct atgcttagat taggggtttt gcaacctgag gtctataaac
     5041 aaagtcttcc tggaagtaat tgtaagcatc ctgaaataaa aaagcaagaa tatgaagaag
     5101 tagttcagac tgttaataca gatttctctc catatctgat ttcagataac ttagaacagc
     5161 ctatgggaag tagtcataca tctcaggttt gttctgagac acctgatgac ctgttagatg
     5221 atggtgaaat aaaggaagat actagttttg ctgaaaatga cattaaggaa agttctgctg
     5281 tttttagcaa aagcgtccag agaggagagc ttagcaggag tcctagccct ttcacccata
     5341 cacatttggc tcagggttac cgaagagggg ccaagaaatt agagtcctca gaagagaact
     5401 tatctagtga ggatgaagag cttccctgct tccaacactt gttatttggt aaagtaaaca
     5461 atataccttc tcagtctact aggcatagca ccgttgctac cgagtgtctg tctaagaaca
     5521 cagaggagaa tttattatca ttgaagaata gcttaaatga ctgcagtaac caggtaatat
     5581 tggcaaaggc atctcaggaa catcacctta gtgaggaaac aaaatgttct gctagcttgt
     5641 tttcttcaca gtgcagtgaa ttggaagact tgactgcaaa tacaaacacc caggatcctt
     5701 tcttgattgg ttcttccaaa caaatgaggc atcagtctga aagccaggga gttggtctga
     5761 gtgacaagga attggtttca gatgatgaag aaagaggaac gggcttggaa gaaaataatc
     5821 aagaagagca aagcatggat tcaaacttag gtgaagcagc atctgggtgt gagagtgaaa
     5881 caagcgtctc tgaagactgc tcagggctat cctctcagag tgacatttta accactcagc
     5941 agagggatac catgcaacat aacctgataa agctccagca ggaaatggct gaactagaag
     6001 ctgtgttaga acagcatggg agccagcctt ctaacagcta ccctagtata atcagtgact
     6061 cttctgccct tgaggacctg cgaaatccag aacaaagcac atcagaaaaa gcagtattaa
     6121 cttcacagaa aagtagtgaa taccctataa gccagaatcc agaaggcctt tctgctgaca
     6181 agtttgaggt gtctgcagat agttctacca gtaaaaataa agaaccagga gtggaaaggt
     6241 catccccttc taaatgccca tcattagatg ataggtggta catgcacagt tgctctggga
     6301 gtcttcagaa tagaaactac ccatctcaag aggagctcat taaggttgtt gatgtggagg
     6361 agcaacagct ggaagagtct gggccacacg atttgacgga aacatcttac ttgccaaggc
     6421 aagatctaga gggaacccct tacctggaat ctggaatcag cctcttctct gatgaccctg
     6481 aatctgatcc ttctgaagac agagccccag agtcagctcg tgttggcaac ataccatctt
     6541 caacctctgc attgaaagtt ccccaattga aagttgcaga atctgcccag ggtccagctg
     6601 ctgctcatac tactgatact gctgggtata atgcaatgga agaaagtgtg agcagggaga
     6661 agccagaatt gacagcttca acagaaaggg tcaacaaaag aatgtccatg gtggtgagtg
     6721 gcctgacccc agaagaattt atgctcgtgt acaagtttgc cagaaaacac cacatcactt
     6781 taactaatct aattactgaa gagactactc atgttgttat gaaaacagat gctgagtttg
     6841 tgtgtgaacg gacactgaaa tattttctag gaattgcggg aggaaaatgg gtagttagct
     6901 atttctgggt gacccagtct attaaagaaa gaaaaatgct gaatgagcat gattttgaag
     6961 tcagaggaga tgtggtcaat ggaagaaacc accaaggtcc aaagcgagca agagaatccc
     7021 aggacagaaa gatcttcagg gggctagaaa tctgttgcta tgggcccttc accaacatgc
     7081 ccacagatca actggaatgg atggtacagc tgtgtggtgc ttctgtggtg aaggagcttt
     7141 catcattcac ccttggcaca ggtgtccacc caattgtggt tgtgcagcca gatgcctgga
     7201 cagaggacaa tggcttccat gcaattgggc agatgtgtga ggcacctgtg gtgacccgag
     7261 agtgggtgtt ggacagtgta gcactctacc agtgccagga gctggacacc tacctgatac
     7321 cccagatccc ccacagccac tacggaggag gtggaagcgg aggaggagga agcggaggag
     7381 gaggtagccg attaataata cgactcacta taggggccac catggactac aaggatgacg
     7441 acgataaacc ctcgacatcc actttgaaac aagttactaa agtggattgt cctgtttgcg
     7501 gggttaacat tccagaaagt cacattaata agcatttaga cagctgttta tcacgcgaag
     7561 agaagaagga aagcctcaga agttctgttc acaaaaggta gacgcgtaaa tgattgcaga
     7621 tccactagtt ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7681 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7741 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7801 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7861 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagatccact
     7921 agttctagcc tcgaggctag agcggccccg gtgcctagag aaggtggcgc ggggtaaact
     7981 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     8041 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     8101 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     8161 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     8221 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     8281 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     8341 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     8401 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     8461 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     8521 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     8581 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     8641 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     8701 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     8761 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     8821 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     8881 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     8941 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     9001 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagcg atcgcatcga
     9061 cagtgtttaa acgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     9121 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc
     9181 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     9241 cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc
     9301 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     9361 caggagcgca ccatcttctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag
     9421 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcgtcgactt caaggaggac
     9481 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacat ctatatcatg
     9541 gccgtcaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa cgtggaggac
     9601 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9661 ctgctgcccg acagccacta cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag
     9721 aagcgcgatc acatggtcct gctggagttc cgcaccgccg ccgggatcac tctcggcatg
     9781 gacgagctgt acaagtgaga attcctagag ctcgctgatc agcctcgact gtgccttcta
     9841 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9901 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9961 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata
    10021 gcaggcatgc gtttaaacat tagtcgacag ggccgccact ggccgtcgtt ttacaacgtc
    10081 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    10141 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    10201 tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    10261 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    10321 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    10381 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    10441 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    10501 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    10561 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    10621 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
    10681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10801 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10861 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10921 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10981 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    11041 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    11101 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    11161 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    11221 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    11281 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    11341 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    11401 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    11461 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    11521 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    11581 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    11641 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11701 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    11761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    11881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    11941 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    12001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    12061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    12121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    12181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    12241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    12301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    12361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    12421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    12481 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    12541 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    12601 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    12661 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    12721 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    12781 aattgtgagc ggataacaat ttcac
//
