LOCUS       sequence-233073-        8765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of LIR mutant TAX1BP1 without its UBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Padman BS, Nguyen TN, Uoselis L, Skulsuppaisarn M, Nguyen LK, 
            Lazarou M
  TITLE     LC3/GABARAPs drive ubiquitin-independent recruitment of Optineurin 
            and NDP52 to amplify mitophagy.
  JOURNAL   Nat Commun. 2019 Jan 24;10(1):408. doi: 10.1038/s41467-019-08335-6.
  PUBMED    30679426
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-24"; volume: "10(1):408. doi"; pages: " 
            10.1038/s41467-019-08335-6"
COMMENT     Sequence Label: pBMN mCherry-TAX1BP1(V142S/Delta-UBD)
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             348..817
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    882..1239
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1304..1720
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1650..1672
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1688..1704
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1730..2104
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2042..2058
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2107..2116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2113..2820
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2258..2276)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2526..2545
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5312..5781
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5911..5928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6082..6670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6162..6181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6834..7625,7626..7694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7457..7476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7695..7799)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  7944..8711
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata aaaatagata
       61 aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt gacaacataa
      121 atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta tgccagtcat
      181 attaattact agtcaattag ttgattttta tttttgacat atacatgtga aagaccccac
      241 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
      301 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      361 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      421 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      481 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      541 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      601 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      661 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      721 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      781 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      841 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      901 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      961 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
     1021 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
     1081 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
     1141 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1201 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1261 ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac
     1321 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
     1381 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
     1441 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
     1501 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     1561 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     1621 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     1681 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
     1741 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
     1801 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
     1861 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
     1921 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1981 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     2041 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     2101 actgccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2161 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2221 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2281 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2341 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2401 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2461 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     2521 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     2581 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     2641 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2701 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     2761 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     2821 tccggactca gatctcgagc tcaagcttgg acatcctttc aagaagtccc attgcagact
     2881 tccaactttg cccatgtcat ctttcaaaat gtggccaaga gttaccttcc taatgcacac
     2941 ctggaatgtc attacacctt aactccatat attcatccac atccaaaaga ttgggttggt
     3001 atattcaagg ttggatggag tactgctcgt gattattaca cgtttttatg gtcccctatg
     3061 cctgaacatt atgtggaagg atcaacagtc aattgtgtac tagcattcca aggatattac
     3121 cttccaaatg atgatggaga attttatcag ttctgttacg ttacccataa gggtgaaatt
     3181 cgtggagcaa gtacaccttt ccagtttcga gcttcttctc cagttgaaga gctgcttact
     3241 atggaagatg aaggaaattc tgacatgtta gtgagcacca caaaagcagg ccttcttgag
     3301 ttgaaaattg agaaaaccat gaaagaaaaa gaagaactgt taaagttaat tgccgttctg
     3361 gaaaaagaaa cagcacaact tcgagaacaa gttgggagaa tggaaagaga acttaaccat
     3421 gagaaagaaa gatgtgacca actgcaagca gaacaaaagg gtcttactga agtaacacaa
     3481 agcttaaaaa tggaaaatga agagtttaag aagaggttca gtgatgctac atccaaagcc
     3541 catcagcttg aggaagatat tgtgtcagta acacataaag caattgaaaa agaaaccgaa
     3601 ttagacagtt taaaggacaa actcaagaag gcacaacatg aaagagaaca acttgaatgt
     3661 cagttgaaga cagagaagga tgaaaaggaa ctttataagg tacatttgaa gaatacagaa
     3721 atagaaaata ccaagcttat gtcagaggtc cagactttaa aaaatataga tgggaacaaa
     3781 gaaagcgtga ttactcattt caaagaagag attggcaggc tgcagttatg tttggctgaa
     3841 aaggaaaatc tgcaaagaac tttcctgctt acaacctcaa gtaaagaaga tacttgtttt
     3901 ttaaaggagc aacttcgtaa agcagaggaa caggttcagg caactcggca agaagttgtc
     3961 tttctggcta aagaactcag tgatgctgtc aacgtacgag acagaacgat ggcagacctg
     4021 catactgcac gcttggaaaa cgagaaagtg aaaaagcagt tagctgatgc agtggcagaa
     4081 cttaaactaa atgctatgaa aaaagatcag gacaagactg atacactgga acacgaacta
     4141 agaagagaag ttgaagatct gaaactccgt cttcagatgg ctgcagacca ttataaagaa
     4201 aaatttaagg aatgccaaag gctccaaaaa caaataaaca aactttcaga tcaatcagct
     4261 aataataata atgtcttcac aaagaaaacg gggaatcagc agaaagtgaa tgatgcttca
     4321 gtaaacacag acccagccac ttctgcctct actgtagatg taaagccatc accttctgca
     4381 gcagaggcag attttgacat agtaacaaag gggcaagtct gtgaaatgac caaagaaatt
     4441 gctgacaaaa cagaaaagta taataaatgt aaacaactct tgcaggatga gaaagcaaaa
     4501 tgcaataaat atgctgatga acttgcaaaa atggagctga aatggaaaga acaagtgaaa
     4561 attgctgaaa atgtaaaact tgaactagct gaagtacagg acaattataa agaacttaaa
     4621 aggagtctag aaaatccagc agaaaggaaa atggaaggtc agaattccca gagtcctcaa
     4681 tgtttcaaaa catgctcaga gcaaaatggt tatgttctca cattgtcaaa tgcacaacca
     4741 gttctgcaat atggtaatcc ttatgcatct caggaaacaa gagatggagc agatggtgct
     4801 ttttacccag atgaaataca aaggccacct gtcagagtcc cctcttgggg actggaagac
     4861 aatgttgtct gcagccagcc tgctcgaaac tttagtcggc ctgatggctt agaggactct
     4921 gaggatagca aagaagatga gaatgtgcct actgctcctg atcctccaag tcaacattta
     4981 cgtgggcatg ggacaggctt ttgctttgat tccagctttg atgttcacaa gaagtgtccc
     5041 ctctgtgagt taatgtttcc tcctaactat gatcagagca aatttgaaga acatgttgaa
     5101 agtcactgga aggtgtgagg atccaccgga tctagataac tgatcacgat aaaataaaag
     5161 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     5221 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     5281 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     5341 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     5401 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     5461 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     5521 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     5581 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     5641 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     5701 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     5761 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
     5821 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg
     5881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6781 gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7681 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7741 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7801 acatttcccc gaaaagtgcc accagctttg ctcttaggag tttcctaata catcccaaac
     7861 tcaaatatat aaagcatttg acttgttcta tgccctaggg ggcgggggga agctaagcca
     7921 gcttttttta acatttaaaa tgtggtaatg actccaactt attgatagtg ttttatgttc
     7981 agataatgcc cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagcgacttc
     8041 cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta
     8101 tatcgcttgc tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca
     8161 tccgtcatcc atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc
     8221 gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct tccggagact gtcatacgcg
     8281 taaaacagcc agcgctggcg cgatttagcc ccgacatagc cccactgttc gtccatttcc
     8341 gcgcagacga tgacgtcact gcccggctgt atgcgcgagg ttaccgactg cggcctgagt
     8401 tttttaagtg acgtaaaatc gtgttgaggc caacgcccat aatgcgggct gttgcccggc
     8461 atccaacgcc attcatggcc atatcaatga ttttctggtg cgtaccgggt tgagaagcgg
     8521 tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga gcagagatag cgctgatgtc
     8581 cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga
     8641 tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg
     8701 gcagcatcac cttaaaatgt taattccatt ttaaatgcac agatgttttt atttcataag
     8761 ggttt
//
