LOCUS       sequence-23308-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Colicelli J, Goff SP
  TITLE     Sequence and spacing requirements of a retrovirus integration site.
  JOURNAL   J Mol Biol. 1988 Jan 5. 199(1):47-59.
  PUBMED    3351923
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1988 Jan 5. 199(1):47-59."
COMMENT     Sequence Label: pNCA
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(171..275)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     343..361
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      301 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      361 ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc atcgattagt ccaatttgtt
      421 aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag
      481 cctatagagt acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg
      541 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
      601 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
      661 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
      721 c
//
