LOCUS       sequence-233109-        6222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of Che9c60 and Che9c61 recombinases on 
            pB264/pBR322 backbone with kanamycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD120
FEATURES             Location/Qualifiers
     source          1..6222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      126..714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     615..634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2436..3230)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2547..2566)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3157..3176
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      3730..3757
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      6089..6135
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
       61 ttcacgaggc agacctcact agttccatga gcgtcagacc ccgtagaaaa gatcaaagga
      121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      541 gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      781 cctgcgagga acgtcgggcg gtcgagcgcg gcgtcgtcgc gtgtggagtt gcgcgagcgg
      841 gtggtcgagg tcgagaaggt ggtgcatcgg ccggccacga cggccattgc tcgtttgaac
      901 aggtctcaca gaccatcagg gtgcaacgca cccgcacggc agggctgtcc caagggggac
      961 gggctggtag tggacgtcat cgcagctgat actcatcgct cgattcctgc cagggcgtga
     1021 tggacagtgc cgaccgagca tcccagctca ttggcgatag ctctcatgga catgccagta
     1081 ccgcgtagct cccgtatctt ctcgcgccgt tgctgtgccc gggacaggta ggcctctcgc
     1141 ggctctgatg tccaccggat gatgctggcc gtcgatacgc ctgtgcgttc ggcgagctcc
     1201 cgcactgtta tcccgttgcg aggcagacgc tcagtcatgt gtggcctcct caattgccag
     1261 tcgcacctga tccagtttgg tgctgcgtgc acgccgggcc tggccgctgg cgacgccgcc
     1321 tttgcggcca cgggcggatt ggatggtcga gagtgtggct tcgtagacgg cgataccgtc
     1381 tttccagatg cgggatcgcg tggtgatcca tcggtgaatg ctggcggcga tggcgcgggc
     1441 ttcagagggt gggagttcct cggtgtagct ggcgttgagg gcggtgactt cgcggtggag
     1501 ggcgagttcg aggccggcgc tgtcatgagt gggcaggtag ttgcgcatga ggccggggcg
     1561 ggtggcccag gtgcgagcgg cgtggaagat tgcgcagttg cggccgagtc ctatgggggc
     1621 gtcacggtgg gccttggtgt gctgccagcg cggggagggc atgtgtgtgc cgagttcggc
     1681 ctcgagctcg gcgaggctgc gcggttcggt gtggatccag tgcgtgtccc agcctgagtg
     1741 tgtggggttc ttggtcatca ggcctgagta gccgatgtcc ccttcgacgg cccggcgtag
     1801 cccttcggta acggcggcgg cgtacgcgag gggcttccgt cgggcgtact cggtgcgggt
     1861 gaacggctcg cgcagccacc agtgcaggtg cgcatgcccg ttgctgctgt tttcgatcac
     1921 ggcgttcggc atagggtgat tgcctgccgc tgacagtgcg cgcagggcgg agtcggggtg
     1981 gtcgacgtcg acgacgagca gattgctcag tgtctgcggg ttggcctcga tgtagcgccg
     2041 ctcaagtgcg gcagggcggc gcattcgata gaccccctca aggaagtcgt ttgttgccaa
     2101 aggccagaac ggtagccaca tttgctccca gtccccacct tcccgcaccc cgtgtgtgtc
     2161 catgctggtg accgtcgcat gctctcgaac gaaacctggg catttccctc ggtgtgttca
     2221 aagttaatcg tgaagccaag tcagatgttt cggtcttaca taaacaacgg cgaagaactc
     2281 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     2341 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     2401 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     2461 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     2521 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     2581 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     2641 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatc
     2701 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     2761 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     2821 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     2881 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     2941 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     3001 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg gagttcattc
     3061 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     3121 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     3181 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     3241 cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag
     3301 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     3361 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     3421 ctgcaagcta cctgctttct ctttgcgctt gcgtctgaca ttcatccggg gtcagcaccg
     3481 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     3541 cttgcggcag cgtgaagcta gcgtctacca ggacttttac ctgtcgaagc tccaagtcag
     3601 atatttcgct gaggggccac cccacaactg cacactcccc cgctctcccg tcgagccctg
     3661 gtggtggaac accagcgaca gccgagcacc cccaaccacc tgtaccaacc actagttgat
     3721 tagctaagca gaaggccatc ctgacggatg gcctttttgc gtttaatact gtttaaactc
     3781 tagaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg
     3841 atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc
     3901 tgtgcgttcg ctgtgcgggc tctaacacgt cctagtatgg taggatgagc aacatttcga
     3961 cgccgagaga ttcgccgccc gaaatgagca cgatccgcat gcttaattaa gaaggagata
     4021 tacatatgag tgtgcccaca caggacggaa tgcaccggtt cgtcgacgag gacgtctacc
     4081 acgctgaccg gggctcgctg tcggtatccg gcgcgaagct gctgttgccg ccgtcgtgtc
     4141 ccgcgaaatt ccgctgggag atggacaaca cccggaagcc gaaaaaggtc tgggacttcg
     4201 gacatgtcgc gcacaaactg gtgctcggca agggtgccga gttcgagatc ctcgaccccg
     4261 aggtgcacgg gctgaaggcg gacggtacgc cgtcggagaa gccgaccgcg acgggcatgt
     4321 ggcgcaaggc cgaggctgag gctcgcaaac agggcaaggt gccgattcac gtcgacctgt
     4381 tcacgaaggc gtacgacatg gccgaaaagg tgcgtcagca cccgacagcc ggcccgatct
     4441 tcgccaatcc tgacggcgag gccgaggtcg cgctgtacta caccgacccc gagaccggcg
     4501 tgcggctgcg tggccggatc gactggctca ctgacgatat cgatgattac aagacgtcga
     4561 tgaccgcgaa cccggccgag ctgaaaacca agttctacaa gctcggctat ttcatgcagg
     4621 cggcctggta catcgatcta ctggtcgccc tcgggctcgc cgagaacccg cgattccggt
     4681 tcatcacgca ggagaaagaa ccgccctacg tcgtgactcc gatccagtac gacgacgagg
     4741 cgatcgaaga ggggcggcgc cgcaaccgcc aggcgatccg gctctacgcc gactgcatgg
     4801 aatcgggcaa gtggcctgac tacagcgacg acgtggtcac gatcagcctg ccctcgtggg
     4861 ggctgccgcg accgcagacc gtcggcgacg tcgtcaccga cagctatatc tacgacaccg
     4921 acccgctcga agaggccgac ccgattgaag gggattacat ctatggctga aaatgctgtc
     4981 accaagcagg attcgcccaa ggcacccgag acgatctcgc aagtgctgca ggtgctcgtg
     5041 ccgcagctgg cgcgtgcagt gcccaagggt atggaccccg accgcatcgc gcggatcgtg
     5101 caaaccgaga tccgcaagtc gcgcaacgcg aaagctgctg gcatcgctaa gcagtccctc
     5161 gacgactgca cgcaagagtc atttgccggt gcgctgctga cctcggccgc gctcggtctc
     5221 gagcccggtg tcaacggcga gtgctacctc gtgccctacc gcgacacccg gcgcggcgtg
     5281 gtcgagtgcc agctgatcat cggctaccag ggcatcgtca aactgttctg gcagcacccg
     5341 cgcgcctccc ggatcgacgc gcagtgggtc ggcgcgaacg acgaattcca ttacaccatg
     5401 ggcctcaatc cgacgctgaa acacgtgaag gccaagggtg atcggggtaa tccggtctac
     5461 ttctacgcga tcgtcgaggt gaccggcgct gagccgctgt gggacgtgtt caccgccgac
     5521 gagatcaggg aattgcgtcg cggcaaggtc ggatcctcgg gcgacatcaa ggacccgcag
     5581 cgctggatgg agcggaagac cgcgctcaaa caggtgctga agctggcacc gaagacgacg
     5641 cggctcgacg cggcgatccg cgccgacgat cgcccgggca ccgacctgtc acagtcgcag
     5701 gcgctcgcgc tgccgtcgac cgtcaagccg acggccgact acatcgacgg cgagatcgcc
     5761 gagccgcacg aggtcgatac gccaccgaag tcgtcgcgcg cacaacgcgc gcagcgcgcc
     5821 accgcgccgg cgcccgacgt gcagatggcc aatcccgatc agctgaagcg cctcggcgag
     5881 atccagaagg ccgaaaagta caacgacgcc gattggttca agttcctcgc cgattcggcc
     5941 ggcgtcaaag ccacgcgcgc cgccgacctc acgttcgacg aggcgaaggc tgtcatcgac
     6001 atgttcgacg ggcccaacgc atgagcgccc cggcgaattc cgacgcggtg gttgatctgc
     6061 aactagcgta cgatcgactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     6121 ggcctttcgt tttatgccat catggccgcg gtgatcagct agccacctga cgtcgggggg
     6181 ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca tt
//
