LOCUS       sequence-233120-        6586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Centromere, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6586)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Rad21-N-18
FEATURES             Location/Qualifiers
     source          1..6586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        91..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        395..598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     549..569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2602..3282
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3404..3525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3441..3460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3495..3514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3532..3987)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3669..3690)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3881..3900
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4014..4118
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4120..4477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4120..4311
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4143..4163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4328..4463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4512..5306
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4512..5306
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4566..4585)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5176..5195
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5494..5513)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5538..5585
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5914..6502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6403..6422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      601 tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgaggc caccatgttc
      661 tacgcacatt ttgttctcag taaaagaggg cctctggcca aaatttggct agcggcccat
      721 tgggataaga agctaaccaa agcccatgtg ttcgagtgta atttagagag cagcgtggag
      781 agtatcatct caccaaaggt gaaaatggca ttacggacat caggacatct cttactggga
      841 gtagttcgaa tctatcacag gaaagccaaa taccttcttg cagactgtaa tgaagcattc
      901 attaagataa agatggcttt tcggccaggt gtggttgacc tgcctgagga aaatcgggaa
      961 gcagcttata atgccattac tttacctgaa gaatttcatg actttgatca gccactgcct
     1021 gacttagatg acatcgatgt ggcccagcag ttcagcttga atcagagtag agtggaagag
     1081 ataaccatga gagaagaagt tgggaacatc agtattttac aagaaaatga ttttggtgat
     1141 tttggaatgg atgatcgtga gataatgaga gaaggcagtg cttttgagga tgacgacatg
     1201 ttagtaagca ctactacttc taacctccta ttagagtctg aacagagcac cagcaatctg
     1261 aatgagaaaa ttaaccattt agaatatgaa gatcaatata aggatgataa ttttggagaa
     1321 ggaaatgatg gtggaatatt agatgacaaa cttattagta ataatgatgg cggtatcttt
     1381 gatgatcccc ctgccctctc tgaggcaggg gtgatgttgc cagagcagcc tgcacatgac
     1441 gatatggatg aggatgataa tgtatcaatg ggtgggcctg atagtcctga ttcagtggat
     1501 cccgttgaac caatgccaac catgactgat caaacaacac ttgttccaaa tgaggaagaa
     1561 gcatttgcat tggagcctat tgatataact gttaaagaaa caaaagccaa gaggaagagg
     1621 aagctaattg ttgacagtgt caaagagttg gatagcaaga caattagagc ccaacttagt
     1681 gattattcag atattgttac tactttggat ctggcaccgc ccaccaagaa attgatgatg
     1741 tggaaagaga caggaggagt agaaaaactg ttttctttac ctgctcagcc tttgtggaat
     1801 aacagactac tgaagctctt tacacgctgt cttacaccgc ttgtaccaga agaccttaga
     1861 aaaaggagga aaggaggaga ggcagataat ttggatgaat tcctcaaaga atttgaaaat
     1921 ccagaggttc ctagagagga ccagcaacag cagcatcagc agcgtgatgt tatcgatgag
     1981 cccattattg aagagccaag ccgcctccag gagtcagtga tggaggccag cagaacaaac
     2041 atagatgagt cagctatgcc tccaccacca cctcagggag ttaagcgaaa agctggacaa
     2101 attgacccag agcctgtgat gcctcctcag caggtagagc agatggaaat accacctgta
     2161 gagcttcccc cagaagaacc tccaaatatc tgtcagctaa taccagagtt agaacttctg
     2221 ccagaaaaag agaaggagaa agagaaggaa aaagaagatg atgaagagga agaggatgaa
     2281 gatgcatcag ggggcgatca agatcaggaa gaaagaagat ggaacaaaag gactcagcag
     2341 atgcttcatg gtcttcagcg tgctcttgct aaaactggag ctgaatctat cagtttgctt
     2401 gagttatgtc gaaatacgaa cagaaaacaa gctgccgcaa agttctacag cttcttggtt
     2461 cttaaaaagc agcaagctat tgagctgaca caggaagaac cgtacagtga catcatcgca
     2521 acacctggac caaggttcca tattataagt cgcggaggca gtggtggggt accgcgggcc
     2581 cgggatccac cggtcgccac catgagtgcg attaagccag acatgaagat caacctccgt
     2641 atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct
     2701 tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc
     2761 tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccga atatccagac
     2821 cacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg
     2881 actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact
     2941 ttctataata aagttcgatt tcacggtgta aactttcccg ccaatggtcc agttatgcag
     3001 aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg
     3061 actggtgata ttcgcatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc
     3121 agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac
     3181 cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat
     3241 gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat
     3301 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     3361 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     3541 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3601 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3661 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3721 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3781 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3841 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3901 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3961 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     4141 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4201 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4381 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4441 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     4501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4681 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     5041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     5341 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5401 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5461 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     5521 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     5581 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     5641 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     5701 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     5761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6541 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgat
//
