LOCUS       sequence-233123-       11467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 11467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti myc-NL-GW
FEATURES             Location/Qualifiers
     source          1..11467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    197..314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        379..1590
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1427..1446
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1628..1657
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1658..2167
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     protein_bind    2174..2298
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2323..2328,2329..2346,2347..2353)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2407..3087
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2473..2492)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3407..3712
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3591..3610
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3753..3877)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3916..4504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3969..3989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4387..4398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4507..4523)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4508..4524)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5029..5209
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5235..5252)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5241..5465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5511..5939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5598..5617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5808..5829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5948..5968)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5953..6282
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6133..6268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6195..6214
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6330..6377
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6396..6770
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6900..7021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6937..6956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6991..7010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7070..7086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7070..7086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7083..7105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7094..7110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7118..7124,7125..7142,7143..7148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7163..7184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7301..7318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7472..8060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7552..7571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8231..9022,9023..9091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8854..8873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9092..9196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9271..9290)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9462..9841
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9843..10041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9996..10016
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10059..10239
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10286..10411
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10904..11137
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11322..11366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
       61 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
      121 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
      181 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      241 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      301 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaacc
      361 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
      421 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
      481 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
      541 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
      601 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
      661 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
      721 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
      781 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
      841 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
      901 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
      961 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     1021 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     1081 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     1141 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     1201 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     1261 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     1321 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     1381 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     1441 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     1501 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     1561 ttctggccgt ttttggcttt tttgttagac gaagcttggg cccgggaatt aattcgctag
     1621 caccatggaa cagaaactga tctctgaaga agacctggtc ttcacactcg aagatttcgt
     1681 tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt
     1741 gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag
     1801 cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg
     1861 cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca
     1921 ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat
     1981 cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt
     2041 aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg
     2101 ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat
     2161 tctggcgaag cttacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     2221 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     2281 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2341 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     2401 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     2461 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     2521 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2581 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2641 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2701 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2761 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2821 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2881 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2941 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     3001 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggcgggg
     3061 cgtaatctag aggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     3121 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     3181 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     3241 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     3301 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     3361 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     3421 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     3481 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     3541 agtcccccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     3601 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     3661 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     3721 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     3781 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     3841 tttacgtttc tcgttcagct ttcttgtaca aagtggtgta actcgagtct agaattcgat
     3901 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3961 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4021 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4081 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4141 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4201 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4261 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4321 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4381 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4441 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4501 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     4561 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     4621 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     4681 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4741 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     4801 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     4861 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     4921 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     4981 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     5041 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5101 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5161 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     5221 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5281 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5341 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5401 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     5461 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     5521 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5581 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5641 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5701 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5761 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5821 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5881 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5941 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6001 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6061 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6181 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     6241 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     6301 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     6361 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     6421 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     6481 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     6541 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     6601 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     6661 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     6721 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     6781 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6841 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6901 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6961 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7021 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7081 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7141 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7201 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7261 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7321 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7381 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7441 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7501 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7561 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7621 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7681 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7741 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7801 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7861 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7921 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7981 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8041 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8101 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8161 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8221 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8281 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8341 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8401 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8461 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8521 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8581 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8641 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8701 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8761 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8821 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8881 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8941 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9001 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9061 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9121 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9181 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     9241 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9301 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     9361 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     9421 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     9481 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     9541 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     9601 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     9661 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     9721 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     9781 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     9841 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     9901 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     9961 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
    10021 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
    10081 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10141 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10201 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    10261 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    10321 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    10381 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    10441 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    10501 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    10561 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    10621 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    10681 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    10741 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    10801 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10861 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10921 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    10981 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    11041 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    11101 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11161 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11221 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    11281 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    11341 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    11401 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    11461 tactttc
//
