LOCUS       sequence-233124-       10698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ABE7.10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Liang P, Xie X, Zhi S, Sun H, Zhang X, Chen Y, Chen Y, Xiong Y, Ma 
            W, Liu D, Huang J, Songyang Z
  TITLE     Genome-wide profiling of adenine base editor specificity by 
            EndoV-seq.
  JOURNAL   Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
  PUBMED    30622278
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-8"; volume: "10(1):67. doi"; pages: " 
            10.1038/s41467-018-07988-z"
COMMENT     Sequence Label: pcDNA3.1(-)-ABE7.10
FEATURES             Location/Qualifiers
     source          1..10698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     635..654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        635..653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             707..1207
                     /codon_start=1
                     /product="tRNA adenosine deaminase from E. coli"
                     /label=TadA
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTD"
     CDS             join(1898..1921,1922..1924,1925..5998)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1898..3382,3383..3385,3386..3874,3875..3877,3878..3976,
                     3977..3979,3980..4669,4670..4672,4673..5998)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Kleinstiver et al., 2016)"
                     /label=SpCas9-HF1
                     /note="carries the mutations N497A, R661A, Q695A, and 
                     Q926A"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6011..6031
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6061..6078)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6067..6291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6337..6765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6424..6443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6634..6655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6774..6794)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6779..7108
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6959..7094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7021..7040
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7175..7969
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7229..7248)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7839..7858
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8143..8264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8180..8199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8234..8253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8313..8329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8313..8329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8326..8348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8337..8353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8361..8367,8368..8385,8386..8391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8406..8427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8544..8561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8715..9303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8795..8814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9474..10265,10266..10334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10097..10116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10335..10439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10514..10533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      601 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      661 aagctggcta gcgtttaaac gggccctcta gactcgagcg gccgccatgt ccgaagtcga
      721 gttttcccat gagtactgga tgagacacgc attgactctc gcaaagaggg cttgggatga
      781 acgcgaggtg cccgtggggg cagtactcgt gcataacaat cgcgtaatcg gcgaaggttg
      841 gaataggccg atcggacgcc acgaccccac tgcacatgcg gaaatcatgg cccttcgaca
      901 gggagggctt gtgatgcaga attatcgact tatcgatgcg acgctgtacg tcacgcttga
      961 accttgcgta atgtgcgcgg gagctatgat tcactcccgc attggacgag ttgtattcgg
     1021 tgcccgcgac gccaagacgg gtgccgcagg ttcactgatg gacgtgctgc atcacccagg
     1081 catgaaccac cgggtagaaa tcacagaagg catattggcg gacgaatgtg cggcgctgtt
     1141 gtccgacttt tttcgcatgc ggaggcagga gatcaaggcc cagaaaaaag cacaatcctc
     1201 tactgactct ggtggttctt ctggtggttc tagcggcagc gagactcccg ggacctcaga
     1261 gtccgccaca cccgaaagtt ctggtggttc ttctggtggt tcttccgagg tcgaattttc
     1321 acatgagtat tggatgcgac acgccttgac gctcgccaaa agggcgaggg acgaacggga
     1381 agttcccgta ggcgccgtcc ttgtactgaa taatcgagtt attggcgaag gttggaacag
     1441 ggccatagga ctgcatgatc caacagccca tgcagaaatc atggcgctcc ggcagggtgg
     1501 ccttgtcatg caaaattata ggctgatcga cgcgacgttg tacgtcacct tcgaaccttg
     1561 cgttatgtgt gcaggcgcta tgatacattc aagaattggg cgagtcgtgt ttggggtcag
     1621 gaacgcaaag actggtgcag ccggttccct tatggatgtg ctccactacc caggaatgaa
     1681 tcatcgggtc gagattacag aggggatact ggctgacgaa tgcgccgccc tcctgtgcta
     1741 cttctttcgg atgcccaggc aggtgtttaa cgcacagaag aaagctcaaa gcagtaccga
     1801 ctctgggggc tctagtggag gctccagcgg ttctgagacc cccggcacta gtgaatctgc
     1861 cactcccgaa tcatccgggg gatcttcagg gggatctgat aaaaagtatt ctattggttt
     1921 agccatcggc actaattccg ttggatgggc tgtcataacc gatgaataca aagtaccttc
     1981 aaagaaattt aaggtgttgg ggaacacaga ccgtcattcg attaaaaaga atcttatcgg
     2041 tgccctccta ttcgatagtg gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg
     2101 gagaaggtat acacgtcgca agaaccgaat atgttactta caagaaattt ttagcaatga
     2161 gatggccaaa gttgacgatt ctttctttca ccgtttggaa gagtccttcc ttgtcgaaga
     2221 ggacaagaaa catgaacggc accccatctt tggaaacata gtagatgagg tggcatatca
     2281 tgaaaagtac ccaacgattt atcacctcag aaaaaagcta gttgactcaa ctgataaagc
     2341 ggacctgagg ttaatctact tggctcttgc ccatatgata aagttccgtg ggcactttct
     2401 cattgagggt gatctaaatc cggacaactc ggatgtcgac aaactgttca tccagttagt
     2461 acaaacctat aatcagttgt ttgaagagaa ccctataaat gcaagtggcg tggatgcgaa
     2521 ggctattctt agcgcccgcc tctctaaatc ccgacggcta gaaaacctga tcgcacaatt
     2581 acccggagag aagaaaaatg ggttgttcgg taaccttata gcgctctcac taggcctgac
     2641 accaaatttt aagtcgaact tcgacttagc tgaagatgcc aaattgcagc ttagtaagga
     2701 cacgtacgat gacgatctcg acaatctact ggcacaaatt ggagatcagt atgcggactt
     2761 atttttggct gccaaaaacc ttagcgatgc aatcctccta tctgacatac tgagagttaa
     2821 tactgagatt accaaggcgc cgttatccgc ttcaatgatc aaaaggtacg atgaacatca
     2881 ccaagacttg acacttctca aggccctagt ccgtcagcaa ctgcctgaga aatataagga
     2941 aatattcttt gatcagtcga aaaacgggta cgcaggttat attgacggcg gagcgagtca
     3001 agaggaattc tacaagttta tcaaacccat attagagaag atggatggga cggaagagtt
     3061 gcttgtaaaa ctcaatcgcg aagatctact gcgaaagcag cggactttcg acaacggtag
     3121 cattccacat caaatccact taggcgaatt gcatgctata cttagaaggc aggaggattt
     3181 ttatccgttc ctcaaagaca atcgtgaaaa gattgagaaa atcctaacct ttcgcatacc
     3241 ttactatgtg ggacccctgg cccgagggaa ctctcggttc gcatggatga caagaaagtc
     3301 cgaagaaacg attactccct ggaattttga ggaagttgtc gataaaggtg cgtcagctca
     3361 atcgttcatc gagaggatga ccaactttga caagaattta ccgaacgaaa aagtattgcc
     3421 taagcacagt ttactttacg agtatttcac agtgtacaat gaactcacga aagttaagta
     3481 tgtcactgag ggcatgcgta aacccgcctt tctaagcgga gaacagaaga aagcaatagt
     3541 agatctgtta ttcaagacca accgcaaagt gacagttaag caattgaaag aggactactt
     3601 taagaaaatt gaatgcttcg attctgtcga gatctccggg gtagaagatc gatttaatgc
     3661 gtcacttggt acgtatcatg acctcctaaa gataattaaa gataaggact tcctggataa
     3721 cgaagagaat gaagatatct tagaagatat agtgttgact cttaccctct ttgaagatcg
     3781 ggaaatgatt gaggaaagac taaaaacata cgctcacctg ttcgacgata aggttatgaa
     3841 acagttaaag aggcgtcgct atacgggctg gggacgcttg tcgcggaaac ttatcaacgg
     3901 gataagagac aagcaaagtg gtaaaactat tctcgatttt ctaaagagcg acggcttcgc
     3961 caataggaac tttatgcagc tgatccatga tgactcttta accttcaaag aggatataca
     4021 aaaggcacag gtttccggac aaggggactc attgcacgaa catattgcga atcttgctgg
     4081 ttcgccagcc atcaaaaagg gcatactcca gacagtcaaa gtagtggatg agctagttaa
     4141 ggtcatggga cgtcacaaac cggaaaacat tgtaatcgag atggcacgcg aaaatcaaac
     4201 gactcagaag gggcaaaaaa acagtcgaga gcggatgaag agaatagaag agggtattaa
     4261 agaactgggc agccagatct taaaggagca tcctgtggaa aatacccaat tgcagaacga
     4321 gaaactttac ctctattacc tacaaaatgg aagggacatg tatgttgatc aggaactgga
     4381 cataaaccgt ttatctgatt acgacgtcga tcacattgta ccccaatcct ttttgaagga
     4441 cgattcaatc gacaataaag tgcttacacg ctcggataag aaccgaggga aaagtgacaa
     4501 tgttccaagc gaggaagtcg taaagaaaat gaagaactat tggcggcagc tcctaaatgc
     4561 gaaactgata acgcaaagaa agttcgataa cttaactaaa gctgagaggg gtggcttgtc
     4621 tgaacttgac aaggccggat ttattaaacg tcagctcgtg gaaacccgcc agatcacaaa
     4681 gcatgttgcg cagatactag attcccgaat gaatacgaaa tacgacgaga acgataagct
     4741 gattcgggaa gtcaaagtaa tcactttaaa gtcaaaattg gtgtcggact tcagaaagga
     4801 ttttcaattc tataaagtta gggagataaa taactaccac catgcgcacg acgcttatct
     4861 taatgccgtc gtagggaccg cactcattaa gaaatacccg aagctagaaa gtgagtttgt
     4921 gtatggtgat tacaaagttt atgacgtccg taagatgatc gcgaaaagcg aacaggagat
     4981 aggcaaggct acagccaaat acttctttta ttctaacatt atgaatttct ttaagacgga
     5041 aatcactctg gcaaacggag agatacgcaa acgaccttta attgaaacca atggggagac
     5101 aggtgaaatc gtatgggata agggccggga cttcgcgacg gtgagaaaag ttttgtccat
     5161 gccccaagtc aacatagtaa agaaaactga ggtgcagacc ggagggtttt caaaggaatc
     5221 gattcttcca aaaaggaata gtgataagct catcgctcgt aaaaaggact gggacccgaa
     5281 aaagtacggt ggcttcgata gccctacagt tgcctattct gtcctagtag tggcaaaagt
     5341 tgagaaggga aaatccaaga aactgaagtc agtcaaagaa ttattgggga taacgattat
     5401 ggagcgctcg tcttttgaaa agaaccccat cgacttcctt gaggcgaaag gttacaagga
     5461 agtaaaaaag gatctcataa ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg
     5521 ccgaaaacgg atgttggcta gcgccggaga gcttcaaaag gggaacgaac tcgcactacc
     5581 gtctaaatac gtgaatttcc tgtatttagc gtcccattac gagaagttga aaggttcacc
     5641 tgaagataac gaacagaagc aactttttgt tgagcagcac aaacattatc tcgacgaaat
     5701 catagagcaa atttcggaat tcagtaagag agtcatccta gctgatgcca atctggacaa
     5761 agtattaagc gcatacaaca agcacaggga taaacccata cgtgagcagg cggaaaatat
     5821 tatccatttg tttactctta ccaacctcgg cgctccagcc gcattcaagt attttgacac
     5881 aacgatagat cgcaaacgat acacttctac caaggaggtg ctagacgcga cactgattca
     5941 ccaatccatc acgggattat atgaaactcg gatagatttg tcacagcttg ggggtgactc
     6001 tggtggttct cccaagaaga agaggaaagt ctaaaagctt aagtttaaac cgctgatcag
     6061 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6121 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6181 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6241 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6301 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6781 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6841 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6901 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7021 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7081 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7141 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     7201 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     7261 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     7321 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     7381 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7441 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7501 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7561 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7621 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7681 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7741 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7801 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7861 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7921 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7981 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8041 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8101 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8161 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8221 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8281 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8341 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8401 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8461 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8521 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8581 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8641 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8701 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8761 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8821 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8881 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8941 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9001 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9061 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9121 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9241 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9301 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9361 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9421 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10441 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    10501 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    10561 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    10621 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    10681 atgtacgggc cagatata
//
